Effects of 0, 0.01, 0.1, 1, 5, and 10 μM of a synthetic strigolactone analogue (GR24) on physiological parameters at day 8 after dark treatment in leaves of perennial ryegrass: (A) chlorophyll content, (B) photochemical efficiency (Fv/Fm), and (C) electrolyte leakage. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor, and a combination of both on physiological parameters at day 8 after dark treatment in leaves of perennial ryegrass: (A) chlorophyll content, (B) photochemical efficiency (Fv/Fm), (C) electrolyte leakage, and (D) phenotype traits. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor, and a combination of both on ethylene production of perennial ryegrass leaves at day 8 after dark treatment. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor, and a combination of both on the relative expression of genes related to senescence including (A) LpSAG12.1, (B) Lph36, and (C) Lpl69 of perennial ryegrass at day 8 after dark treatment. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor), and a combination of both on the relative expression of (A) chlorophyll degradation-associated genes (LpNYC1, LpNOL, LpPPH, LpPAO, LpRCCR1, and LpSGR) and (B) chlorophyll biosynthesis-associated genes (LpHEMA, LpGSA, LpHEMB, LpHEME, LpCHLH, and LpCHLG) of perennial ryegrass at day 8 after dark treatment. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
(A) Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor), and a combination of both on the relative expression of SL biosynthesis-related genes LpD17 and SL signaling-associated genes (LpD3 and LpD14) of perennial ryegrass after dark treatment. (B) Effects of GR24, AVG, and a combination of both on the relative expression of ethylene biosynthesis-related genes (LpACO) and ethylene signaling-associated genes (LpEIN4 and LpERS1) of perennial ryegrass after dark treatment. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
Click on author name to view affiliation information
Accelerated or premature leaf senescence induced by dark conditions could be associated with chlorophyll degradation and regulated by hormones. To study the effects of strigolactone (SL) on dark-induced leaf senescence and to examine the interaction effects of SL and ethylene on regulating dark-induced leaf senescence, plants of perennial ryegrass (Lolium perenne) exposed to darkness for 8 days were treated with a synthetic SL analogue (GR24), aminoethoxyvinyl glycine [AVG (an ethylene biosynthesis inhibitor)], or SL and AVG by foliar spray. Chlorophyll content, photochemical efficiency, electrolyte leakage, and ethylene production were measured. Expressions of genes associated with leaf senescence, SL biosynthesis and signaling, ethylene biosynthesis and signaling, and chlorophyll biosynthesis and degradation were determined. Foliar application of GR24 promoted leaf senescence in perennial ryegrass grown in darkness, and the intensity of action increased with the GR24 concentration. SL-accelerated leaf senescence was associated with the downregulation of four chlorophyll biosynthesis-associated genes and upregulation of four chlorophyll degradation-associated genes. AVG had functions counteractive to SL, suppressing dark-induced leaf senescence by downregulating chlorophyll degradation genes and SL synthesis genes. Our results suggested that SL and ethylene interactively regulated leaf senescence, mainly by controlling chlorophyll degradation induced by darkness in perennial ryegrass.
Leaf senescence is regulated by hormones and is also inducible by environmental stresses, such as low light (Wingler et al., 1998). Strigolactone (SL) was first discovered as a stimulant of seed germination in some plant species, such as witchweed [Striga lutea (Cook et al., 1966)] and broomrape [Orobanche ramose (Xie et al., 2010)]. Recent research found that SL is a plant hormone that has multiple roles in plant development, like promoting seed germination (Toh et al., 2012), altering plant architecture (Hu et al., 2018; Umehara et al., 2008), and enhancing plant stress tolerance to drought, salt, heat, phosphate deficiency, and nitrate deficiency (Ha et al., 2014; Hu et al., 2018; Sun et al., 2014). Several studies reported that SL may also be involved in regulating leaf senescence. Mutant plants deficient in SL biosynthesis or insensitive to SL signaling like max2 and d3 showed delayed leaf senescence compared with the wild type in several plant species (Liu et al., 2013; Snowden et al., 2005, 2012; Woo et al., 2001; Yan et al., 2007). The application of a synthetic SL analogue (GR24) promoted leaf senescence in SL-deficient mutants like d27, d17, and d10 in rice (Oryza sativa) and max1, max3, and max4 in arabidopsis (Arabidopsis thaliana), but not in SL-signaling mutants like d3 and d14 in rice or max2 and atd14 in arabidopsis (Ueda and Kusaba, 2015; Yamada et al., 2014). However, there is no report of the altered senescence phenotype for SL mutants or transgenic plants in pea (Pisum sativum) or tomato (Solanum lycopersicum) (Beveridge et al., 1997; Kohlen et al., 2012; Vogel et al., 2010). Several senescence-associated genes, such as SAG12.1, h36, and l69, are known as typical senescence markers, which are positively related to leaf senescence (Lee et al., 2001; Zhang et al., 2016; Zhou et al., 2013). Results of SL effects on leaf senescence are incomplete in the literature. Furthermore, there is limited information regarding the mechanisms of SL regulation of leaf senescence.
Leaf senescence is manifested by the decrease in chlorophyll caused by the decline in chlorophyll synthesis (Vajpayee et al., 2000) or the acceleration of chlorophyll degradation (Jespersen et al., 2016; Zhang et al., 2011). Chlorophyll degradation is controlled by multiple genes, including chlorophyllide a oxygenase (NYC1), chlorophyll b reductase (NOL), pheophytinase (PPH), pheophorbide a oxygenase (PAO), red chlorophyll catabolite reductase (RCCR1), and nonyellowing 1 (SGR). Chlorophyll biosynthesis is regulated by multiple genes, including glutamyl-tRNA reductase (HEMA), glutamate-1-semialdehyde 2,1- aminotransferase (GSA), 5-aminolevulinate dehydrogenase (HEMB), uroporphyrinogen III decarboxylase (HEME), Mg-chelatase (CHLH), and chlorophyll synthase (CHLG) (Yu et al., 2018; Zhu et al., 2015). Qiu et al. (2015) reported that ethylene insensitive3 (EIN3) was an important transcription factor in the ethylene-signaling pathway that promoted chlorophyll degradation via the direct increase of chlorophyll catabolic genes, such as NYC1 and PAO, in arabidopsis. However, whether leaf senescence affected by SL is due to inhibited chlorophyll synthesis genes and/or promoted chlorophyll degradation genes and senescence marker genes is not well-documented.
We hypothesized that SL may affect leaf senescence in perennial ryegrass (Lolium perenne) exposed to dark conditions by affecting chlorophyll biosynthesis and/or degradation independently or interactively with ethylene. Therefore, the objectives of this study were to examine the effects of SL on dark-induced leaf senescence and the chlorophyll metabolism of perennial ryegrass by an exogenous application of GR24 and to investigate the interactive effects of SL and ethylene in regulating leaf senescence in perennial grass exposed to dark conditions by exogenous treatment with an ethylene inhibitor, aminoethoxyvinyl glycine (AVG).
Seeds of perennial ryegrass (cv. Pangaea) were planted in 30 plastic pots (12 × 12 cm) filled with potting mix including sphagnum peatmoss and humus (ProMix; Premier Horticulture, Quakertown, PA) on 10 Apr. 2018. Plants were maintained in growth chambers (Environmental Growth Chambers, Chagrin Falls, OH) controlled at 400 μmol·m−2·s−1 photosynthetically active radiation at temperatures of 22/18 °C (day/night) with 60% relative humidity for a 14-h photoperiod. All plants were well-watered and fertilized weekly with half-strength Hoagland’s nutrient solution (Hoagland and Arnon, 1950).
To examine the effective concentrations of SL affecting dark-induced leaf senescence in perennial ryegrass, 4-week-old seedlings were sprayed with either distilled water (control) or solution containing 0.01, 0.1, 1, 5, or 10 μM rac-GR24 (dissolved in acetone and diluted with water) before the exposure of plants to darkness. At each application, 40 mL of solution was sprayed onto leaves in each pot. The experiment was arranged as a completely randomized design with three replicates (three pots) for each treatment. Five pots of plants treated with five concentrations of GR24 were randomly placed in each of three growth chambers. The pots were relocated across and within the growth chamber every 2 d to avoid environmental variations in different chambers
To induce leaf senescence, plants treated with or without GR24 of different concentrations were exposed to dark conditions in growth chambers with lights off for 8 d. To determine whether ethylene was involved in SL effects on dark-induced leaf senescence in perennial ryegrass, 4-week-old plants were treated with distilled water (control), 10 μM GR24 (concentration was optimized by a pre-experiment), 5 μM AVG (dissolved in water), or both GR24 and AVG on 12 May 2018, before plants were exposed to darkness. The experiment was arranged as a completely randomized design with three replicates (three pots of plants with multiple plants in each pot) for each treatment. Four pots of plants treated with water, GR24, AVG, or both GR24 and AVG were randomly placed in each of three growth chambers. The pots were relocated across and within the growth chamber every 2 d to avoid environmental variations in different chambers.
Fully expanded leaves were sampled on 20 May 2018, to measure the physiological parameters. Leaf chlorophyll content, photochemical efficiency (Fv/Fm), and electrolyte leakage (EL) were determined as physiological index measurements. Chlorophyll content was measured using leaves soaked in dimethyl sulfoxide (DMSO) in darkness for 72 h to extract chlorophyll. Then, a spectrophotometer (721G; Shanghai Jingke Instrument Plant, Shanghai, China) was used to measure the absorbance of extracts at 663 and 645 nm. Chlorophyll content was calculated according to the equations published previously (Barnes et al., 1992). Fv/Fm was measured using a fluorescence meter (PAM-2500; Walz, Effeltrich, Germany) as described by Oxborough and Baker (1997). To measure EL, 0.2 g of leaves were sampled. The leaves were washed three times and then immersed in deionized water (35 mL). After shaking for 24 h, initial conductivity (Ci) was determined with a conductivity meter (DDS-307A; Shanghai Jingke Instrument Plant). The maximum conductivity (Cmax) was measured after the leaves were boiled for 20 min. The EL was determined as 100 × Ci/Cmax (Murray et al., 1989).
To measure ethylene production in leaves exposed to GR24 and AVG treatments, the blades of fully expanded leaves of perennial ryegrass were excised from whole plants that were treated with distilled water (control), 10 μM GR24, 5 μM AVG, or both GR24 and AVG and exposed to darkness for 8 d. Leaf samples were wrapped in paper towels moistened with distilled water, GR24, AVG, or both GR24 and AVG; then, they were placed in an airtight 20-mL glass vial for 2 h for the release of ethylene from leaves. A 100-μL gas sample was collected from each vial using a syringe and injected in a gas chromatograph (GC) (6890N; Agilent Technologies, Santa Clara, CA) to analyze the ethylene concentration following the procedure described by Zaidi et al. (2016). The system consisted of a GC column, pre-concentrator (PC), and SnO2 detector, with PC heated to 250 °C for ethylene release.
The transcript levels of genes related to senescence, chlorophyll, ethylene, and SL in leaves were analyzed by a quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). Total RNA was extracted from leaf tissue with E.Z.N.A. Plant RNA Kit (Omega Bio-tek, Norcross, GA), and contaminating genomic DNA was removed with TURBO DNA-free reagent (Life Technologies, Grand Island, NY). Next, 2 μg of total RNA was reverse-transcribed to cDNA using the high-capacity cDNA reverse-transcription kit (Life Technologies). During the gene expression analysis, a real-time PCR system (CFX CONNCT; Bio-Rad, Hercules, CA) was used and the PCR reaction was performed with a power SYBR Green PCR Master mix (Applied Biosystems, Foster City, CA). Primers used in this study are listed in Table 1, and the data were standardized using eIF4A (Huang et al., 2014). The relative expression level between genes of interest and the reference gene was calculated using the ΔΔCt method. All reactions were performed with two technical and three biological replicates.
Effects of GR24 treatment and AVG treatment on all parameters were analyzed by an analysis of variance with SPSS software (version 20.0; IBM, Armonk, NY). Differences between mean values for each parameter were determined by a least significance test with P = 0.05.
GR24 treatments lead to significant decreases in leaf chlorophyll content and photochemical efficiency (Fig. 1A and B) and increases in EL (Fig. 1C) in perennial ryegrass exposed to darkness. The effects were more pronounced with increasing GR24 concentrations (Fig. 1). Leaves treated with GR24 appeared yellower compared with the untreated control (Fig. 2). Treatment with AVG alone and with AVG combined with GR24 maintained greener leaves compared with the GR24 treatment (Fig. 2). The AVG application ameliorated the senescence traits caused by GR24, causing 15.9% and 36.0% increases in chlorophyll content and photochemical efficiency, respectively (Fig. 2A and B), and a 4.1% decrease in EL compared with the GR24 treatment (Fig. 2C).
Citation: Journal of the American Society for Horticultural Science J. Amer. Soc. Hort. Sci. 146, 2; 10.21273/JASHS04933-20
Citation: Journal of the American Society for Horticultural Science J. Amer. Soc. Hort. Sci. 146, 2; 10.21273/JASHS04933-20
To determine whether SL-accelerated leaf senescence is associated with ethylene production, ethylene evolution rates of leaves treated with GR24 or AVG were examined. As shown in Fig. 3, AVG inhibited ethylene production, causing a 78.9% decrease in the ethylene evolution rate. However, GR24 promoted ethylene production, with a 320% increase compared to the untreated control. The ethylene production rate was also significantly greater with the combined AVG and GR24 treatment compared with the untreated control, but it was significantly lower than that of plants treated with GR24 alone.
Citation: Journal of the American Society for Horticultural Science J. Amer. Soc. Hort. Sci. 146, 2; 10.21273/JASHS04933-20
The transcript changes of three genes related to leaf senescence (LpSAG12.1, Lph36, and Lpl69) were compared among plants treated with or without GR24 or AVG. The relative expressions of all three genes were significantly increased in plants treated with GR24 alone, with a 201.3% increase for LpSAG12.1, 369.0% increase for Lph36, and 210.3% increase for Lpl69 (Fig. 4A–C). The AVG treatment caused downregulation of LpSAG12.1 but had no significant effects on Lph36, and Lpl69. The expression levels were significantly lower in plants treated with both AVG and GR24 compared with those treated with GR24 alone; levels were 87.2% lower for LpSAG12.1, 25.5% lower for Lph36, and 12.3% lower for Lpl69 (Fig. 4A–C).
Citation: Journal of the American Society for Horticultural Science J. Amer. Soc. Hort. Sci. 146, 2; 10.21273/JASHS04933-20
To investigate whether GR24 or AVG may affect chlorophyll degradation and/or suppress chlorophyll biosynthesis, the different expression levels of six chlorophyll degradation-associated genes (LpNYC1, LpNOL, LpPPH, LpPAO, LpRCCR1, and LpSGR) and six chlorophyll biosynthesis-associated genes (LpHEMA, LpGSA, LpHEMB, LpHEME, LpCHLH, and LpCHLG) were compared between plants treated with or without GR24, AVG, or a combination of GR24 and AVG. The relative expression levels of four chlorophyll degradation-associated genes (LpNOL, LpPPH, LpPAO, and LpRCCR1) were increased with GR24 treatment (Fig. 5A), and the relative expression of all six chlorophyll degradation-associated genes were decreased with AVG treatment (Fig. 5A). Expression levels were significantly lower in plants treated with both AVG and GR24 compared with those treated with GR24 alone; levels were 61.5% lower for LpNYC1, 34.2% lower for LpNOL, 34.6% lower for LpPPH, 39.4% lower for LpPAO, 29.1% lower for LpRCCR1, and 61.3% lower for LpSGR (Fig. 5A).
Citation: Journal of the American Society for Horticultural Science J. Amer. Soc. Hort. Sci. 146, 2; 10.21273/JASHS04933-20
The relative expression of four chlorophyll biosynthesis-associated genes decreased with GR24 treatment compared with the untreated control, with a 42.5% decrease for LpHEMA, 18.9% decrease for LpGSA, 34.7% decrease for LpHEMB, and 32.9% for LpCHLH (Fig. 5B). AVG treatment significantly enhanced the relative expression of all six chlorophyll biosynthesis-associated genes. The expression levels of four chlorophyll biosynthesis-associated genes did not differ significantly between GR24 treatment and the combined AVG and GR24 treatment (Fig. 5B).
To understand changes in SL-related genes and ethylene-related genes in response to GR24 or AVG, the expression levels of SL biosynthesis-associated gene LpD17, SL signaling-associated genes LpD3 and LpD14, ethylene biosynthesis-associated gene LpACO, and ethylene signaling-associated genes LpEIN4 and LpERS1 were analyzed. The relative expression levels of LpD17, LpD3, and LpD14 increased with GR24 treatment (133%, 244%, and 214% increase, respectively), but they decreased significantly with AVG treatment (39.7%, 30.2%, and 56.4%, respectively) (Fig. 6A). The relative expression of ethylene-related genes increased with GR24 treatment, with an 81.4% increase for LpACO, 27.2% increase for LpEIN4, and 35.9% increase for LpERS1 (Fig. 6B). AVG treatment suppressed the relative expression compared with the untreated control, with a 48.5% decrease for LpACO, 26.4% decrease for LpEIN4, and 59.5% decrease for LpERS1 (Fig. 6B). Plants treated with the combined AVG and GR24 treatment had significantly lower relative expression levels that those treated with GR24 alone, with 71.3% lower levels for LpEIN4 and 66.0% lower levels for LpERS1 (Fig. 6B).
Citation: Journal of the American Society for Horticultural Science J. Amer. Soc. Hort. Sci. 146, 2; 10.21273/JASHS04933-20
The physiological analysis showed that the exogenous GR24 application effectively accelerated leaf senescence in perennial ryegrass incubated in the dark, as characterized by the lower chlorophyll content, lower photochemical efficiency, and higher EL. The trend in expressions of senescence-related genes LpSAG12.1, Lph36, and Lpl69 also support this conclusion.
Ethylene acts as a positive regulator of leaf senescence (Jibran et al., 2013), and ethylene production has been considered an important factor contributing to senescence of leaves (Yang and Hoffman, 1984). SL may interact with ethylene, affecting leaf senescence (Kapulnik et al., 2011; Li et al., 2018; Ueda and Kusaba, 2015). In our studies, ethylene production rates increased significantly with the GR24 treatment, whereas the combined AVG and GR24 treatment decreased the production compared with the GR24 treatment. Ethylene biosynthesis-associated gene LpACO and ethylene signaling-associated genes LpEIN4 and LpERS1 showed a similar trend (Fig. 6B). These data support the notion that SL could regulate dark-induced leaf senescence by promoting ethylene synthesis and signaling in perennial ryegrass. Kapulnik et al. (2011) suggested that SL promotes root hair elongation by enhancing ethylene production. Additionally, Sugimoto et al. (2003) reported that SL causes upregulated production of ethylene by inducing mRNA expression of ethylene biosynthesis-associated genes during germination of Striga hermonthica. Another study reported that the addition of AVG suppressed germination induced by SL in S. hermonthica (Logan and Stewart, 1991), which is consistent with the leaf senescence results found during our study. These studies suggested that the synergistic effects of SL and ethylene may be consistent during the development of plants.
A transcript analysis of SL biosynthesis and signaling-associated genes demonstrated that AVG application downregulated the expression of SL biosynthesis-associated gene LpD17, which is consistent with a previous study of arabidopsis (Kapulnik et al., 2011; Li et al., 2018; Ueda and Kusaba, 2015). This indicated that ethylene also promoted SL biosynthesis in a monocotyledon plant such as perennial ryegrass. In addition, the expression of SL signaling-associated genes, including LpD3 and LpD14, were also repressed with AVG application. Together, these results suggest that an SL-dependent pathway also contributes to ethylene-induced senescence.
In our study, GR24 treatment resulted in reduced expression of four chlorophyll biosynthesis-associated genes and upregulation of four chlorophyll degradation-associated genes in perennial ryegrass grown in the dark. Upregulation of chlorophyll degradation-associated genes caused by SL could be ameliorated by AVG treatment, but AVG had no obvious antagonistic effects on SL for chlorophyll biosynthesis-associated genes. These results indicated that SL may have an adverse effect on chlorophyll biosynthesis-associated genes and a positive effect on chlorophyll degradation-associated genes, thus leading to a lower chlorophyll content. However, the decrease could be relieved by AVG through suppressing SL upregulation of chlorophyll degradation-associated genes. The expression levels of PAO, PPH, and RCCR genes associated with chlorophyll degradation were enhanced with ethylene treatment in chinese flowering cabbage [Brassica rapa var. parachinensis (Zhang et al., 2011)] and broccoli [Brassica oleracea var. italica (Buchert et al., 2011)]. Ethylene has been shown to create a several-fold increase in chlorophyllase activity that accelerates chlorophyll breakdown (Amir-Shapira et al., 1987; Purvis and Barmore, 1981; Shimokawa et al., 1978); however, chlorophyll synthesis was slightly inhibited by ethylene treatment (Alscher and Castelfranco, 1972). Based on all of these studies, we suggest that SL promotes leaf senescence through the acceleration of chlorophyll degradation in concert with ethylene. Moreover, SL is involved in an ethylene-independent, senescence-promoting pathway through downregulating chlorophyll synthesis under dark conditions.
We found that exogenously applied GR24 promotes leaf senescence in the dark in perennial ryegrass, and that the intensity of action increased with the GR24 concentration. SL-accelerated leaf senescence was associated with the downregulation of four chlorophyll biosynthesis-associated genes and upregulation of four chlorophyll degradation-associated genes. AVG had functions counteractive to SL, thus suppressing leaf senescence by downregulating chlorophyll degradation genes and SL synthesis genes. Our results suggest that SL and ethylene interactively regulated leaf senescence, mainly by controlling chlorophyll degradation induced by darkness in perennial ryegrass. However, the underlying mechanisms of whether and how SL and ethylene directly interact with each other to control stress-induced leaf senescence deserve further investigation.
Effects of 0, 0.01, 0.1, 1, 5, and 10 μM of a synthetic strigolactone analogue (GR24) on physiological parameters at day 8 after dark treatment in leaves of perennial ryegrass: (A) chlorophyll content, (B) photochemical efficiency (Fv/Fm), and (C) electrolyte leakage. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor, and a combination of both on physiological parameters at day 8 after dark treatment in leaves of perennial ryegrass: (A) chlorophyll content, (B) photochemical efficiency (Fv/Fm), (C) electrolyte leakage, and (D) phenotype traits. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor, and a combination of both on ethylene production of perennial ryegrass leaves at day 8 after dark treatment. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor, and a combination of both on the relative expression of genes related to senescence including (A) LpSAG12.1, (B) Lph36, and (C) Lpl69 of perennial ryegrass at day 8 after dark treatment. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor), and a combination of both on the relative expression of (A) chlorophyll degradation-associated genes (LpNYC1, LpNOL, LpPPH, LpPAO, LpRCCR1, and LpSGR) and (B) chlorophyll biosynthesis-associated genes (LpHEMA, LpGSA, LpHEMB, LpHEME, LpCHLH, and LpCHLG) of perennial ryegrass at day 8 after dark treatment. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
(A) Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor), and a combination of both on the relative expression of SL biosynthesis-related genes LpD17 and SL signaling-associated genes (LpD3 and LpD14) of perennial ryegrass after dark treatment. (B) Effects of GR24, AVG, and a combination of both on the relative expression of ethylene biosynthesis-related genes (LpACO) and ethylene signaling-associated genes (LpEIN4 and LpERS1) of perennial ryegrass after dark treatment. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
Contributor Notes
This research was supported by Rutgers Center for Turfgrass Science, National Key R&D Program of China [2016YFC0500202], and Qinling National Forest Ecosystem Research Station of China. We thank Stephanie Rossi and William Errickson at Rutgers University for critical review and editing of the manuscript.
B.H. is the corresponding author. E-mail: huang@sebs.rutgers.edu.
Effects of 0, 0.01, 0.1, 1, 5, and 10 μM of a synthetic strigolactone analogue (GR24) on physiological parameters at day 8 after dark treatment in leaves of perennial ryegrass: (A) chlorophyll content, (B) photochemical efficiency (Fv/Fm), and (C) electrolyte leakage. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor, and a combination of both on physiological parameters at day 8 after dark treatment in leaves of perennial ryegrass: (A) chlorophyll content, (B) photochemical efficiency (Fv/Fm), (C) electrolyte leakage, and (D) phenotype traits. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor, and a combination of both on ethylene production of perennial ryegrass leaves at day 8 after dark treatment. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor, and a combination of both on the relative expression of genes related to senescence including (A) LpSAG12.1, (B) Lph36, and (C) Lpl69 of perennial ryegrass at day 8 after dark treatment. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor), and a combination of both on the relative expression of (A) chlorophyll degradation-associated genes (LpNYC1, LpNOL, LpPPH, LpPAO, LpRCCR1, and LpSGR) and (B) chlorophyll biosynthesis-associated genes (LpHEMA, LpGSA, LpHEMB, LpHEME, LpCHLH, and LpCHLG) of perennial ryegrass at day 8 after dark treatment. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
(A) Effects of a synthetic strigolactone analogue (GR24), aminoethoxyvinyl glycine (AVG), which is an ethylene biosynthesis inhibitor), and a combination of both on the relative expression of SL biosynthesis-related genes LpD17 and SL signaling-associated genes (LpD3 and LpD14) of perennial ryegrass after dark treatment. (B) Effects of GR24, AVG, and a combination of both on the relative expression of ethylene biosynthesis-related genes (LpACO) and ethylene signaling-associated genes (LpEIN4 and LpERS1) of perennial ryegrass after dark treatment. Bars represent the se for three biological replicates. The lettering indicates significant differences when treatments were marked with different letters according to the Duncan multiple range test (P ≤ 0.05).
