Search Results

You are looking at 1 - 10 of 636 items for :

  • woody plant medium x
  • Refine by Access: All x
Clear All
Full access

Danny L. Barney, Omar A. Lopez, and Elizabeth King

) reported on the effects of plant growth regulators on in vitro culture and microshoot rooting of mountain huckleberry, although those trials were limited to modified woody plant medium (WPM) ( Lloyd and McCown, 1980 ). Reed and Abdelnour-Esquivel (1991

Free access

Wenhao Dai and Cielo Castillo

photoperiod. Plant regeneration Experiments were conducted to determine factors affecting plant regeneration of Buddleia . Unless otherwise stated, all regeneration media are woody plant medium (WPM) ( Lloyd and McCown, 1980 ) supplemented with MS

Free access

Tracy S. Hawkins, Nathan M. Schiff, Emile S. Gardiner, Theodor Leininger, Margaret S. Devall, Dan Wilson, Paul Hamel, Deborah D. McCown, and Kristina Connor

twice in sterile distilled water. Sterilized explants were placed on woody plant medium (WPM) ( Lloyd and McCown, 1980 ) supplemented with 1 μ m zeatin, and with a mixture of agar (4 g·L −1 ) and Gelright (1.4 g·L −1 ) added as gelling agents. Explants

Open access

Toktam Taghavi, Alireza Rahemi, Reza Rafie, and Maru K. Kering

and changed the PGRs combinations and concentrations. A review listed full-strength MS as the primary medium ( Prabhakaran Nair, 2013 ), but others have also been tested [half-strength MS ( Sit and Tiwari, 1996 ), Lloyd & McCown woody plant basal (L

Free access

Seong Min Woo and Hazel Y. Wetzstein

thiamine HCl, 100 mg·L −1 myo-inositol, 20 g·L −1 sucrose, and 4 g·L −1 Gel-Gro with Woody Plant Medium (WPM) ( Lloyd and McCown, 1980 ) with the pH adjusted to 5.2. Young leaves, older leaves, and bud explants were prepared as described in sterilization

Full access

Andrea N. Brennan, Valerie C. Pence, Matthew D. Taylor, Brian W. Trader, and Murphy Westwood

–2 weeks. Fig. 2. Nutrient comparison of Lloyd and McCown woody plant medium ( Lloyd and McCown, 1980 ) and Gressoff and Doy medium ( Gresshoff and Doy, 1972 ). Data in figure were taken from the medium product labels (PhytoTech Laboratories); NO 3

Free access

Ze Li, Xiaofeng Tan, Zhiming Liu, Qing Lin, Lin Zhang, Jun Yuan, Yanling Zeng, and Lingli Wu

than MS medium (data not shown). Regeneration of strong and healthy shoots is the key step in plant rooting. After 30 d in culture, the adventitious shoots were excised and inoculated for elongation. The length of shoots and number of leaves were

Free access

Andrea Swanberg and Wenhao Dai

. Plant regeneration. Experiments were conducted to determine factors affecting plant regeneration of periwinkle cultivars. Unless otherwise stated, all basal regeneration media were woody plant medium (WPM) ( Lloyd and McCown, 1980 ) supplemented with

Free access

Chandra Thammina, Mingyang He, Litang Lu, Kaishuang Cao, Hao Yu, Yongqin Chen, Liangtao Tian, Junmei Chen, Richard McAvoy, Donna Ellis, Degang Zhao, Yuejin Wang, Xian Zhang, and Yi Li

diploids. That approach is time-consuming and technically difficult, especially for some woody species. Endosperm in angiosperms is naturally triploid and in vitro culture of endosperm explants has been used as a method for producing triploid plants

Open access

Doina Clapa, Claudiu Bunea, Orsolya Borsai, Adela Pintea, Monica Hârța, Răzvan Ştefan, and Alexandru Fira

; Zhang et al., 2006 ). The results showed that the most suitable medium for this species was McCown’s Woody Plant Basal Salt Mixture, or Woody Plant Medium (WPM), as described by Lloyd and McCown (1980) . In the following years, the influence of