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Danielle R. Ellis and Kathryn C. Taylor

A partial cDNA (cvzbp-1) was cloned based on the N-terminal sequence of a citrus (Citrus L.) vascular Zn-binding protein (CVZBP) previously isolated from vascular tissue (Taylor et al., 2002). CVZBP has homology to the Kunitz soybean proteinase inhibitor (KSPI) family. Recombinant protein produced using the cDNA clone inhibited the cysteine proteinase, papain. Metal binding capacity has not been reported for any other member of this family. CVZBP was present in leaves, stems, and roots but not seeds of all citrus species examined. However, CVZBP was present in germinating seeds after the cotyledons had turned green. Within four hrs after wounding, CVZBP was undetectable in the wounded leaf and adjacent leaves. It has been suggested that many members of the KSPI family serve a function in defense. However, the expression of the CVZBP is in direct contrast with those of KSPI members that were implicated in defense response. Though systemically regulated during wounding, we suggest that CVZBP is not a defense protein but rather may function in vascular development.

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Thomas E. Marler, Anders Lindström and Jack B. Fisher

stems have concentric cylinders of vascular tissue that contain soft, parenchymatous xylem tissue and little lignified tissue with each new additional cylinder differentiating at the base of the stem between the cortex and the youngest pre

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Jack B. Fisher, Anders Lindström and Thomas E. Marler

in woody stems of other gymnosperms (e.g., conifers, Gnetum , and Ginkgo ). Cycad stems are also unusual in having concentric cylinders of vascular tissue ( Fig. 1A ) that are produced by multiple vascular cambia with each new additional cylinder

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Huanfang Liu, Chelsea D. Specht, Tong Zhao and Jingping Liao

and radial division (arrows) in the pericycle with vascular tissues generated from it in young rhizome. ( H ) Vascular bundles collateral in a ring in the pericycle. ( I ) Vascular bundles collateral in a ring and scattered more in pith than cortex in

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Simon A. Mng'omba, Elsa S. du Toit, Festus K. Akinnifesi and Helena M. Venter

stocks with almost similar stem diameter and bark thickness. This improves proximity of vascular tissues of the scions and stocks. In this trial, bark thickness at the union was not measured, but could be a factor contributing to an increase in union

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Uttara C. Samarakoon, Keith A. Funnell, David J. Woolley, Barbara A. Ambrose and Ed R. Morgan

exogenously from more superficial tissue like the epidermis ( Evert, 2006 ). In the current study, these differences between adventitious and axillary buds, their location of formation, presence of gaps in the vascular ring, vascular connections to the primary

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Michele R. Warmund

hypertrophy in response to larval feeding ( Dreger-Juaffret and Shorthouse, 1992 ). A layer of nutritive tissue forms adjacent to the larval chamber and vascular tissue within the gall joins that of the host organ. During the maturation stage, the last larval

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Jaroslav Ďurkovič, Ingrid Čaňová, Lucia Javoříková, Monika Kardošová, Rastislav Lagaňa, Tibor Priwitzer, Roman Longauer and Jana Krajňáková

-section (scale bars = 400 μm). ( B, D ) Mesophyll tissue, cross-section (scale bars = 100 μm). Leaf histology and midrib vascular traits. Midrib sections (0.4 × 0.4 cm), excised from the leaf base, were fixed in 5% (v/v) glutaraldehyde in a 0.1 m cacodylate

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Olfa Zarrouk, Pilar S. Testillano, María Carmen Risueño, María Ángeles Moreno and Yolanda Gogorcena

were mainly expressed in cambial and vascular tissues. The major differences found in the histological study at the interface level, related to cambium and vascular organization, are summarized in Table 3 . Because the percentage of similarity of all

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J. Naalamle Amissah, Dominick J. Paolillo Jr and Nina Bassuk

formation of secondary vascular tissue with a sinuous outline ( Fig. 1A ). There was a distinct five-lobed pith ( Fig. 1A ) with lobes extending into the area of the vascular tissue that curved outward, accounting for the sinuous outline of the vascular