A method was developed to measure transpiration rates and apparent water-vapor permeability coefficients (P'H2O) of detached fruit using an analytical balance equipped with a humidity chamber, wide-range humidity-generating and sensing devices, and a datalogger. The system was designed to monitor weight changes with time and, hence, weight loss of individual fruit during exposure to specific relative humidities (RHs) and temperatures. Weight loss was corrected for loss due to respiratory exchange of 02 and CO2 before calculating P'H2O. Values of P'H2O for tomatoes obtained using this method over periods of 5 minutes to 24 hours ranged from 3 to 12 nmol·cm-2·s-1·kPa-1 at 20C, depending on the experimental conditions. These values are similar to previously published values and to those obtained in a conventional weight-loss experiment, which involved intermittent weighing. P'H20 for tomatoes dropped ≈15% in 24 hours. P'H20 increased with a transient increase in RH; the extent of the increase was variable from fruit to fruit, ranging from 5% to 100% over 30% to 90% RH. The change was reversible in that P'H2O increased and decreased within minutes following shifts in RH. Similar changes were found for strawberry P'H20. The increase in P'H2O may be due, in part, to a direct effect of water vapor on the water transport properties of the cuticular polymer and surface temperature depression as a result of evaporative cooling. At 50% RH and 20C, water vapor diffuses from tomatoes 50 times faster than O2 enters on a molar basis. This information will be useful for modeling RH changes in modified-atmosphere packages.
Ahmad Shirazi and Arthur C. Cameron
Keun Ho Cho, Beyoung Hwa Kwack, Moo Ryong Huh, and Chiwon W. Lee
The biomass yield, transpiration rate, and chlorophyll contents in Cymbidium goeringii plants grown under various light, temperature, and humidity conditions were investigated. Two-year-old plants potted in pine-bark medium were grown for 12 weeks during the summer months in polyethylene film-covered mini-greenhouses having four different environmental conditions: a) closed house (CH) with high humidity (95.1% RH), high light (800 μmol·m–2·s–1) and high temperature (37.5 °C), b) ventilated house (VH) with low humidity (41.4% RH), high light (800 μmol·m–2·s–1), and medium temperature (31.5 °C), c) shaded closed house (SCH) with high humidity (91.0% RH), low light (110 μmol·m–2·s–1) and medium temperature (33.3 °C), and d) shaded ventilated house (SVH) with medium humidity (61.5% RH), low light (110 μmol·m–2·s–1) and low temperature (30.5 °C). Plants grown in CH produced leaf chlorosis with 50% shorter leaves and 40% lower relative growth rate (7.9 mg/g fresh weight per day) compared to plants grown in SVH. Cymbidium plants grown in SCH or SVH showed higher leaf and root dry weights as compared to those grown in CH or VH. Leaf chlorophyll-a and -b contents as well as carbohydrate levels were the highest in plants grown in SVH, indicating the benefits of shading and ventilation. The rate of transpiration showed a quadratic response to increasing levels of leaf temperature (r 2 = 0.81), wind velocity (r 2 = 0.82), and vapor pressure deficit (VPD, r 2 = 0.91). Regression analysis revealed that the maximum transpiration rate occurred at 25.4 °C leaf temperature, 2.1 m/s wind velocity, and 2.3 kPa VPD in this experiment.
Michael Raviv, J. Heinrich Lieth, David W. Burger, and Rony Wallach
Physical characteristics of two media were studied concerning water availability to roots, as reflected in specific transpiration rate, stomatal conductance, and specific growth rate of very young leaflets of `Kardinal' rose (Rosa ×hybrida L.), grafted on Rosa canina L. `Natal Brier'. Plants were grown in UC mix [42% composted fir bark, 33% peat, and 25% sand (by volume)] or in coconut coir. Water release curves of the media were developed and hydraulic conductivities were calculated. Irrigation pulses were actuated according to predetermined media moisture tensions. Transpiration rate of plants was measured gravimetrically using load cells. Specific transpiration rate (STR) was calculated from these data and leaf area. STR and stomatal conductance were also determined using a steady-state porometer. Specific growth rate (RSG) of young leaflets was calculated from the difference between metabolic heat rate and respiration rate, which served as an indicator for growth potential. Low STR values found at tensions between 0 and 1.5 kPa in UC mix suggest this medium has insufficient free air space for proper root activity within this range. Above 2.3 kPa, unsaturated hydraulic conductivity of UC mix was lower than that of coir, possibly lowering STR values of UC mix-grown plants. As a result of these two factors, STR of plants grown in coir was 20% to 30% higher than that of plants grown in UC mix. STR of coir-grown plants started to decline only at tensions around 4.5 kPa. Yield (number of flowers produced) by coir-grown plants was 19% higher than UC mix-grown plants. This study demonstrated the crucial role of reaching sufficient air-filled porosity in the medium shortly after irrigation. It also suggests that hydraulic conductivity is a more representative measure of water availability than tension.
A. Naor and S. Cohen
The sensitivity of water stress indicators to changing moisture availability, and their variability, determine the number of measurements that should be taken in order to represent properly plant water status in a certain orchard. In the present study we examined the sensitivity and variability of maximum daily trunk shrinkage, midday stem water potential, and daily transpiration rate in their responses to withholding irrigation from field-grown drip-irrigated `Golden delicious' apple trees in a commercial orchard. Irrigation was withheld from the stressed trees for 17 days starting in mid-July, and the control trees were irrigated daily at 100% of the “Class A” pan evaporation rate. The courses of daily transpiration rate, maximum trunk shrinkage, and midday stem water potential before and 10 days after the drying period were similar in the control and the stressed trees. Highly significant differences between the stressed and the control trees in their midday stem water potentials were apparent from the early stages of the stress period. Daily transpiration rate and maximum daily shrinkage were more variable than midday stem water potential, and differences between treatments became significant only after measurements were expressed relative to the initial values before irrigation was witheld. Differences between treatments (as percentages of the values obtained for the control trees) increased after irrigation stopped where these differences were greatest for maximum daily shrinkage, which reached 90%; moderate for stem water potential (60%); and least for daily transpiration rate, for which the differences remained below 20%. Our data show that the choice of a certain water stress indicator should be based on both the sensitivity to changing moisture availability and the degree of variability. Possible reasons for the different sensitivity to moisture availability and the different variability between the water stress indicators under study are discussed.
Ian Merwin and Warren C. Stiles
Eight vegetation management systems (VMS) were evaluated over four years in a newly planted apple site. VMS treatments included pre- and post-emergence herbicide strips, a close-mowed sodgrass, a growth-suppressed (maleic-hydrazide) sodgrass, a crownvetch “living mulch,” clean cultivation, and straw mulch. Soil moisture supply was highest under the straw mulch and lowest under crownvetch, and varied inversely with groundcover biomass. Leaf N was deficient in tress in both sodgrass VMS, and increased by the lequme “living mulch” only after four years. Leaf Cu was lowest, and appeared to limit tree growth in VMS with prolonged soil moisture deficits. No significant differences were observed in leaf transpiration over a broad range (10 to 700 kPa) of soil matric tension. Cumulative trunk crosssectional area was greatest in straw-mulched trees and least in sodgrass and crownvetch VMS. The optimal soil matric tension for nutrient uptake and tree growth appeared to be 175 to 200 kPa in this orchard. Increasing the width of glyphosate herbicide strips from 1.5 to 2.5 m in tree rows did not improve tree growth, nutritional status or fruit yield.
Craig D. Green, Ann Stodola, and Robert M. Augé
Mycorrhizal colonization can alter stomatal behavior of host leaves during drought. This may be related to an altered production or reception of a chemical signal of soil drying. We tested whether intact root systems were required to observe a mycorrhizal effect on leaf transpiration (E), or whether some residual mycorrhizal influence on leaves could affect E of foliage detached from root systems. Transpiration assays were performed in the presence of several possible candidates for a chemical signal of soil drying. In detached leaves of Vigna unguiculata (cowpea), colonization interacted significantly with ABA and pH in regulating transpiration. Colonization affected E of detached Rosa hybrida (rose) leaves but had no effect on E of detached leaves of Pelargonium hortorum (geranium). In each species tested, increasing the ABA concentration decreased E. In cowpea, calcium appeared to alter stomatal sensitivity to ABA, as well as regulate stomatal activity directly. The pH of the feeding solution affected E in rose, but did not change E independently in cowpea or geranium. Adding phosphorus to the feeding solution did not alter E or the apparent sensitivity of stomata to ABA in any of the test species. Colonization of roots by mycorrhizal fungi can result in residual effects in detached leaves, that can alter the stomatal reception of chemical signals in both rose and cowpea.
Thomas O. Athoo, Andreas Winkler, and Moritz Knoche
pedicels) is usually described by Fick’s law: where F (grams per second) is the measured rate of transpiration, A (square meters) the organ’s surface area, and ΔC (grams per cubic meter) the difference in water vapor concentration between the inside
Will Wheeler, Reagan Wytsalucy, Brent Black, Grant Cardon, and Bruce Bugbee
drought tolerance of modern orchards, requiring less irrigation. Weighing lysimeters provide a reliable method of applying drought stress because the transpiration rate of an entire tree can be determined over short intervals, summed over a day and
Georgene L. Johnson, Thomas R. Sinclair, and Kevin Kenworthy
drought tolerance. A high volumetric soil water content at which transpiration rate begins to decrease would be a possible mechanism to conserve soil water and maintain turf quality for a longer time as drought conditions persist. Identification of
Lance V. Stott, Brent Black, and Bruce Bugbee
with no leaching is due to the uptake and transpiration of water from the root zone. Weighing lysimeters, thus, provide a way to determine whole-tree transpiration rates over hours, days, and weeks ( Ben-Gal et al., 2010 ). The precision offered by