, 2007 ). The expression of SDH genes varied depending on the fruit tissue. Expression of SDH2 was cortex-specific, that of SDH6 and SDH9 were seed-specific, and SDH1 and SDH3 were shared by both tissues. Thus, immature leaves exhibited the
Dehydrins are desiccation-induced proteins. Many plants have several dehydrin genes, some of which are primarily cold induced while others are primarily abscisic acid (ABA) or desiccation induced. Only one dehydrin gene (ppdhn1) has been reported in peach. The dehydrin gene is seasonally regulated and associated with cold acclimation. Because molecular markers for desiccation resistance may aid in the selection of drought- and cold-tolerant genotypes, we sought to determine if ppdhn1 was inducible by desiccation and ABA in all tissues (i.e., a whole-plant response) and to examine the relationship between expression of ppdhn1, desiccation, and dehydrin protein (PCA60). One-year-old `Rio Oso Gem' peach [Pranus persica (L.) Batsch.] trees were maintained at a stem water potential of -2.0 MPa by withholding water for 1 week, followed by daily watering for 1 week for some of the trees. ABA (100 mm) was applied to similar trees that were well watered. Total RNA and protein were extracted from bark, leaves, xylem, and roots, fractionated by electrophoresis, blotted to membranes, and probed with either a peach-specific dehydrin cDNA clone or polyclonal antibodies directed against dehydrin. Accumulation of ppdhn1/PCA60 was induced more by desiccation than ABA applications. Additionally, such accumulation was tissue dependent, being highest in bark tissues and lowest in leaf tissues. The presence of ppdhn1 transcript and corresponding PCA60 protein were not always commensurate with each other. In particular, elevated levels of PCA60 were still present 1 week after desiccation recovery when transcript levels had decreased significantly or were undetectable, indicating that dehydrin is a stable protein. In general, our data indicate that ppdhn1 is similar to other cold-induced dehydrins that are only slightly induced by ABA. In contrast to cold-induced dehydrins, ppdhn1 was strongly induced by desiccation. While synthesis of dehydrin is tightly associated with the onset of stress, disappearance and turnover seem less linked to alleviation of the inducing stress.
of the genes encoding these enzymes have been cloned and share high sequence similarity across species and typically exhibit tissue- or development-specific expression. Six enzymes are generally involved in anthocyanin biosynthesis. Chalcone
( Otani and Shimada, 2002 ; Otani et al., 2003 ; Wakita et al., 2001 ). To obtain sufficient expression levels of transgenes in target tissues of genetically engineered sweetpotato plants, the selection of promoters is crucial. To date, few tissue-specific
the endocarp hardened ( Fig. 5 ). To verify that the observed lignin pathway gene expression was associated with the endocarp tissue, PAL, C4H, CCoAOMT, and PR expression was monitored in specific tissue to assess the spatial pattern of gene expression
white; the labellum contains areas of deep anthocyanin pigmentation. Because the labellum produces anthocyanins, the lack of anthocyanin in the petals and sepals must be the result of differential tissue-specific gene expression. Comparisons of
genome (Raymond et al., 2018). In this study, we identified the RcDof family genes based on the Rosa chinensis genome, and their gene structures, conserved domains, and tissue-specific expression were comprehensively analyzed. Our findings provide a
tissues and at all developmental stages. However, some ClR2R3-MYB genes exhibited tissue-specific expression patterns. Twelve ClR2R3-MYB genes including Cla007815 , Cla007846 , Cla015834 , Cla013461 , Cla018610 , Cla021474 , Cla018967 , Cla
the promoter regions of these genes were analyzed, and used quantitative reverse transcription polymerase chain reaction (qRT-PCR) to analyze the expression of nine SLB genes in various tissues and under different abiotic stresses. In this study, we
also exist on chromosomes, and the MCScanX software can help to confirm that a group of multiple pairs of sequences is generated by the replication of another group (i.e., there is one fragment duplication event). Tissue-specific expression