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Andrea N. Brennan, Valerie C. Pence, Matthew D. Taylor, Brian W. Trader, and Murphy Westwood

oak species in the world ( Oldfield and Eastwood, 2007 ), it would be valuable to predict oak tissue culture responses by taxonomic group and establish protocols that could be generalized across related species. The first objective was to determine

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Carrie A. Radcliffe, James M. Affolter, and Hazel Y. Wetzstein

's safeguarding nursery. The tissue culture protocol developed for georgia plume was highly effective in producing adventitious shoots in a broad range of genotypes originating from divergent populations. The use of leaf tissue provides a source of highly

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Miles Schwartz Sax, Nina Bassuk, and Mark Bridgen

stool-bed rooting method. To overcome this limitation, tissue culture protocols were trialed to determine if in vitro methods could successfully be used to clonally propagate UHI hybrid oaks. The use of oak tissue culture methods used to grow plants

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Seong Min Woo and Hazel Y. Wetzstein

for the future. Georgia plume has sustained a severe loss of habitat from the loss of forests in agricultural production. Tissue culture can be an excellent approach for propagation and conservation of threatened or endangered species ( Fay, 1992

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Seong Min Woo and Hazel Y. Wetzstein

as blueberry, cranberry, and rhododendron. This has led to the development of efficient plant regeneration protocols achieved through organogenesis from cultures derived from leaf tissue, shoot tips, and axillary buds. Plants regenerated from this

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Aekaterini N. Martini, Maria Papafotiou, and Stavros N. Vemmos

methods, which are supposed to return tissue explants from the mature to juvenile phase and include application of cytokinin either during or immediately after explants are placed in culture, serial grafting, propagation of stump sprouts, or severe pruning

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Muhammad Irshad, Hafiz Muhammad Rizwan, Biswojit Debnath, Muhammad Anwar, Min Li, Shuang Liu, Bizhu He, and Dongliang Qiu

cotton micropropagation with 0.2 mg·L −1 GA 3 alone in MS medium. However, in our study, the shoot elongation protocol was improved with the combination of BA and GA 3 . Similar effects of the BA and GA 3 combination in tissue culture medium were also

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Beatrice Nesi, Debora Trinchello, Sara Lazzereschi, Antonio Grassotti, and Barbara Ruffoni

culture, and in vitro thermotherapy. Further efficiency of detection by ELISA and RT-PCR as influenced by genetic lines is discussed. Material and Methods Plant material and in vitro culture conditions. To set up an in vitro multiplication protocol, bulb

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Xiuli Shen and Myeong-Je Cho

of cold stratification, chemical scarification, GA 3 treatment, and artificial opening on seed germination. The results of this study can be applied to establish a tissue culture and genetic transformation protocol for sugar pine. Materials and

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Jane Kahia, Peter Kanze Sallah, Lucien Diby, Christophe Kouame, Margaret Kirika, Simeon Niyitegeka, and Theodore Asiimwe

the year irrespective of the season. This study aimed to developing an efficient and rapid propagation protocol for C. betacea plant through tissue culture techniques. An increasing number of studies describing in vitro propagation of Tamarillo using