-made subunit vaccine against pneumonic and bubonic plague is orally immunogenic in mice Vaccine 24 2477 2490 Alvarez, M.L. Pinyerd, H.L. Topal, E. Cardineau, G.A. 2008 P19-dependent and P19-independent
transformed with the Y. pestis f1-v fusion gene encoding the F1-V fusion protein, a subunit vaccine candidate against bubonic and pneumonic plague, and driven by the constitutive cauliflower mosaic virus (CaMV) 35S promoter. They demonstrated with mice that
Transgenic plants and plant viruses have potential advantages over other production systems for therapeutic proteins. 1) Plants are not susceptible to human and animal pathogens, such as viruses that can contaminate mammalian and avian cell lines used for production of many vaccines. Recent experiences of “mad cow” disease and theories of the possible origin of HIV from monkey cell lines have highlighted the need for increased product safety. 2) There are established protocols for preparing naturally occurring pharmaceuticals from plants. 3) Unlike bacteria, plants recognize the same glycosylation signals as other eukaryotic expression systems such as mammalian, insect, or yeast cell cultures and can thus produce glycosylated proteins. Although there are differences between plants and other eukaryotes in the types of sugar residues added to glycosylated proteins, it has been demonstrated several times that plant-produced proteins have similar stability and bioequivalence of function and that antigenicity is similar. 4) Plants can produce high yields; a single transgenic plant could yield as much human glucocerebrosidase as 500 placentae. We expressed an epitope from HIV-1 on the surface of bean yellow mosaic potyvirus (BYMV) coat protein (CP); protein produced in transgenic plants is recognized by a human monoclonal antibody that neutralizes most HIV-1 isolates. Epitope-modified BYMV-CP can be recovered from transgenic plants by incorporation into BYMV virions following infection of the transgenic plants. Modified virions display the HIV-1 epitope in a semi-regular array that should stimulate the immune system to a greater degree than free subunits. HIV epitope-bearing BYMV has been used to immunize mice to assess the immune response.
.M. Pinkhasov, J. Walmsley, A.M. Mason, H.S. Cardineau, G.A. 2006 Plant-made subunit vaccine against pneumonic and bubonic plague is orally immunogenic in mice Vaccine 24 2477 2490 Alvarez, M.L. Pinyerd, H.L. Topal, E. Cardineau, G.A. 2008 P19-dependent and P19
tomato so far for expression of various subunit vaccines, including those against respiratory syncytial virus ( Sandhu et al., 2000 ), cholera ( Jani et al., 2002 ), Norwalk virus ( Huang et al., 2005 ; Zhang et al., 2006 ), plague ( Alvarez et al., 2006
the effect of a high electrical conductivity (EC) of hydroponic nutrient solution as a factor that may affect the protein concentration in fruit of transgenic tomato expressing a candidate subunit vaccine against plague (F1-V). They found that the high
activity of saponins from Kazakhstani plants on the immune responses to subunit influenza vaccine Arch. Virol. 162 3817 3826 10.1007/s00705-017-3560-5 Tyler, V.E. 2000 Herbal medicine: From the past to the future Public Health Nutr. 3 4a 447 452