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M. Gómez Tena, M.A. Pedreño, A. Ros Barceló, and M.A. Ferrer

The subcellular localization of a basic peroxidase (EC 1.11.1.7) isoenzyme in crisphead lettuce (Lactuca sativa L.) leaves was studied through subcellular fractionation and protoplast and vacuole isolation. This isoenzyme is mainly located in soluble fractions. Studies using protoplast isolation and vacuole purification indicated that the soluble basic peroxidase isoenzyme is found in the vacuolar sap, probably in equilibrium with the same isoenzyme attached to tonoplast membranes.

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Elizabeth A. Bihn and Robert J. Ferl

The 14-3-3 proteins were originally characterized in mammalian brains and were thought to be specifically involved in neurotransmitter production. Subsequent research has revealed that this family of proteins is ubiquitous in eucaryotic cells and is involved in a wide range of regulatory and signal transduction pathways. For instance, some 14-3-3 proteins have been associated with the signal transduction in response to fungal pathogen attack and to other environmental factors that affect transcription. In Arabidopsis, 10 isoforms of 14-3-3 have been isolated, raising the possibility that diversity of function may be governed by cellular and subcellular specificities of expression and localization. We have investigated the localization of certain 14-3-3 isoforms through transgenic expression of epitope-tagged 14-3-3s.

Open access

Lili Dong, Tongrui Liu, Di Gao, Jing Li, and Jie Qian

cloned the petunia homolog gene of SDG8 , carried out bioinformatics analysis on its sequence, and detected the expression level of PhSDG8 in different tissues using quantitative real-time PCR (qRT-PCR). Also its subcellular localization and function

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Shuguang Wang, Yongpeng Ma, Chengbin Wan, Chungyun Hse, Todd F. Shupe, Yujun Wang, and Changming Wang

and transport, and the characteristics of their dynamic spatial changes during shoot elongation are quite limited, necessitating further investigations. The subcellular localization of endogenous IAA and its role in shoot elongation remain unclear. In

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Shutian Tao, Danyang Wang, Cong Jin, Wei Sun, Xing Liu, Shaoling Zhang, Fuyong Gao, and Shahrokh Khanizadeh

., 2004 ). The subcellular location of eukaryotic proteins was predicted using the Softberry program ( Iskandar et al., 2014 ). Subcellular localization of PbrC4H. The accurately sequenced recombinants described above were selected and then plasmids were

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Yumei Zhang, Runfang Hu, Huawei Li, Haisheng Zhu, Jinming Zhao, Na Guo, Han Xing, and Guoqiang Lin

annotated as their biological function according to Bevan et al. (1998) . The subcellular localization prediction of 48 differently abundant proteins was based on Plant-PLoc ( Chou and Shen, 2008 ) ( http://www.csbio.sjtu.edu.cn/bioinf/plant/ ). Results

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Xiaobo Sun, Yanming Deng, Lijian Liang, Xinping Jia, Zheng Xiao, and Jiale Su

), small basic intrinsic proteins (SIPs), and uncharacterized intrinsic proteins (XIPs) according to subcellular localization and sequence similarity ( Johanson et al., 2001 ; Lopez et al., 2012 ; Sade et al., 2009 ). These various AQPs are involved in

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Ruigang Wu, Yi Wang, Ting Wu, Xuefeng Xu, and Zhenhai Han

–6 min at 25 °C, after which the supernatant was discarded, and the bacterial pellet was resuspended in 10 m m MgCl 2 to its original titer. Subcellular localization of MdMYB4. The complete MdMYB4 open reading frame was PCR-amplified using primers

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Zhigang Ouyang, Huihui Duan, Lanfang Mi, Wei Hu, Jianmei Chen, Xingtao Li, and Balian Zhong

sets of RNA). Subcellular localization. The coding sequences of the CitYTH genes were amplified by PCR with specific primers ( Supplemental Table 1 ). The PCR products were then digested with Xba I and Sam I restriction enzymes and subsequently

Open access

Xiaoying Dou, Jinrong Bai, Huan Wang, Ying Kong, Lixin Lang, Fang Bao, and Hongzhong Shang

784468), LhDFR (BAM28975), and LhANS (BAM28976). Determination of protein subcellular localization. The LhWDR open reading frame (ORF) lacking stop codon was amplified with rTaq using the primers LhWDR-CDS-F and LhWDR-CDS-R ( Table 1 ). After