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Carlos Calderón-Vázquez, Mary L. Durbin, Vanessa E.T.M. Ashworth, Livia Tommasini, Kapua K.T. Meyer, and Michael T. Clegg

compounds that are known to confer a health benefit (carotenoids, β-sitosterol, and vitamin E). Avocados are a significant source of dietary phytosterols, in particular β-sitosterol, that has been shown to decrease cholesterol absorption in the intestine

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Ray F. Dawson

Diosgenin is a steroidal aglycone occurring in certain species of Dioscorea native principally to eastern Mexico. In the 1940s, diosgenin became a much-sought-after intermediate for the chemical synthesis of certain corticosteroids and structurally related fertility regulants. Various difficulties of access to native sources led to attempts at plantation production. One of these, supported by the Upjohn Company between 1962 and 1980, was located on the Pacific coast of Guatemala and is described herein from the standpoint of technology development. The Dioscorea plant produces a long, coarse vine that requires support. The deep-growing, fleshy rhizome contains the diosgenin and, at harvest, must be removed from soil depths up to 1 m. Dry rhizome yield depends on supply of readily available (low-tension) soil water. Sites located over abundant water reserves give satisfactory rhizome yields, but diosgenin concentrations fall to uneconomically low levels under such circumstances. By 1980, diosgenin had been displaced by two products of soya oil processing, stigmasterol and sitosterol, which became available as a result of advances in microbial fermentation technology. Consequently, the cultivation of Dioscorea was abandoned.

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Álvaro Fernández-Cuesta, Ossama Kodad, Rafel Socias i Company, and Leonardo Velasco

the composition of almond phytosterols. Although all the studies have identified β-sitosterol as the predominant phytosterol in almond, they differ in the proportions of the other phytosterols. Several studies ( Dulf et al., 2010 ; Normén et al., 2007

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Vanessa E.T.M. Ashworth, Haofeng Chen, Carlos L. Calderón-Vázquez, Mary Lu Arpaia, David N. Kuhn, Mary L. Durbin, Livia Tommasini, Elizabeth Deyett, Zhenyu Jia, Michael T. Clegg, and Philippe E. Rolshausen

anticarcinogenic properties ( D’Ambrosio, 2007 ; Ding et al., 2009 ; Lopez-Ledesma et al., 1996 ) conferred by three main groups of compounds: β-sitosterol, carotenoids, and vitamin E. Most of these phenotypic traits are inherited in a quantitative fashion; i

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Shiva Ram Bhandari, Bo-Deul Jung, Hum-Young Baek, and Young-Sang Lee

sugars, total phenols, total flavonoids, vitamin E, squalene, campesterol, β-sitosterol, stigmasterol, and fatty acid composition, in pepper fruits and their relationships with antioxidant activity in five pepper cultivars most popularly cultivated in

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Shiow Y. Wang and Miklos Faust

The changes of membrane lipids in apple (Malus domestics Borkh. cv. Delicious) auxillary and terminal buds from August to April were determined. The predominant lipids were monogalactosyl diglyceride (MGDG), digalactosyl diglyceride (DGDG), phosphatidylcholine (PC), and phosphatidylethanolamine (PE). An increase in membrane polar lipids was associated with budbreak and bud growth from August to April. Linolenic acid was the predominant fatty acid in MGDG, DGDG, and PC, while linoleic acid was predominant in PE. Phosphatidylglycerol (PG) and phosphatidylinositol (PI) contained a high amount of palmitic acid. The ratio of (18:2 + 18:3) to 18:1 fatty acids in galactolipids in apple buds increased from August to April. ß-Sitosterol and sitosteryl ester were the predominant sterols in apple buds. An increase in sitosterol, a decrease in sitosteryl ester, and a decline in the ratio of free sterols to phospholipids occurred during budbreak in spring. A decrease in sitosterol was associated with bud expansion in spring.

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Bruce D. Whitaker

Lipid composition and pigment content were determined in pericarp of `Pik Red' tomatoes (Lycopersicon esculentum Mill.) that were harvested when mature-green (MG) then ripened for 1 or 14 days at 20C, chilled for 11 or 21 days at 2C, or chilled for 21 days and transferred to 20C for 4 days (rewarmed). During ripening, chlorophyll fell below a detectable level, carotenes increased 100-fold, phospholipids (PLs) dropped ≈20%, and galactolipids (GLs) dropped ≈35%. Fatty-acid unsaturation decreased slightly. Steryl esters (SEs), more than free sterols (FSs) and steryl glycosides (SGs), increased at the expense of acylated steryl glycosides (ASGs), and in all four steryl lipids, the stigmasterol: sitosterol ratio rose dramatically, whereas the level of isofucosterol fell sharply. During chilling, chlorophyll declined ≈40% and carotenes ≈60%. PL content did not change, whereas GL fell ≈15%. Fatty-acid unsaturation increased slightly. FS, much more than SG and SE, increased at the expense of ASG. The stigmasterol: sitosterol ratio changed little in ASG, SG, and SE but declined in FS. Isofucosterol increased in FS and SE. Rewarming had little effect on the levels of chlorophyll, carotenes, or PL levels, but caused GL to fall another ≈15%. Fatty-acid unsaturation decreased slightly in GL and ASG. The distribution of total sterol in ASG, SG, FS, and SE changed dramatically, yielding proportions close to those in unchilled MG fruit. Also, 4 days after rewarming, the stigmasterol: sitosterol ratio had increased sharply, particularly in FS and SE, and there was a further rise in isofucosterol in all four steryl lipids. These results indicate that chloroplast damage occurs during chilling, but PL-rich cell membranes are not degraded, even upon rewarming. Changes in sterol composition and conjugation during chilling and after rewarming could result in membrane dysfunction.

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Bruce D. Whitaker

Altered metabolism of membrane lipids has been proposed as a mechanism for the beneficial effects of postharvest calcium treatment on apple quality. A previous study showed that after transfer of apples stored 6 months at 0C to 20C, calcium-treated fruit exhibited slower loss of galactolipid and altered levels of sterol conjugates. The present study of lipids in “control” fruit was conducted as a prelude to further in-depth analyses of the effects of postharvest calcium and heat treatments on lipid metabolism in apples during and after cold storage. Neutral lipid, glycolipid (GL), and phospholipid (PL) fractions were obtained by column chromatography followed by TLC separation of GL and PL classes. The major GL were steryl glycosides (SG), acylated steryl glycosides (ASG), cerebrosides (CB), and mono- and digalactosyl diacylglycerols. Phosphatidylcholine (PC) > P-ethanolamine (PE) > P-irositol (PI) were the major PL. The fatty acids of PC and PE were quite similar, whereas those of PI were more saturated. CB included only 2-hydroxy fatty acids. Among the steryl lipids, free sterols > SG > ASG, with beta-sitosterol >90% of the total sterol in each.

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Bruce D. Whitaker

A previous study of lipids from pericarp tissue of tomato fruit ranging from mature-green to red-ripe showed a large increase in total sterols accompanied by dramatic changes in sterol composition and conjugation with ripening. This study was conducted to determine whether similar changes occur in microsomal membranes derived from tomato fruit pericarp. Acylated steryl glycoside (ASG), the predominant steryl lipid, declined during ripening, with increases in steryl glycoside (SG) and free sterol (FS). Only minor changes in fatty acid composition were associated with the drop in ASG. The stigmasterol:sitosterol ratio increased throughout ripening, but much more in Fs than in SG or ASG. The ratio of FS to phospholipid (PL) increased with ripening. However, FS was never greater than 10 percent of the total membrane sterol (TMS), and TMS:PL actually declined over the middle stages of ripening. It is not known why tomato tissues maintain such high levels of ASG and SG, but sterol conjugation is thought to regulate the physical properties of cell membranes.

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Bruce D. Whitaker

MG tomato fruit were stored for four or 12 days at chilling (2C) or nonchilling (15C) temperature. Fruits stored 12 days at 15C ripened to the turning stage, whereas fruits at 2C did not ripen. Lipids of microsomes and plastids from pericarp tissue were analyzed at harvest and after four or 12 days of storage. After 12 days at either 15C or 2C, the ratio of phospholipid (PL) to protein in microsomes declined, with a concomitant increase in the ratio of total membrane sterols (TMS) to PL. The TMS/PL ratio also increased in crude plastids. In both microsomes and plastids, free sterols (FS) increased more at 2C than at 15C, and thus accounted for a larger percentage of the TMS. The ratio of stigmasterol to sitosterol in steryl lipids, particularly in FS, increased more at 15C than at 2C. The unsaturation index of fatty acids in PL and galactolipids generally increased slightly during storage at both 15C and 2C. The ratio of phosphatidylethanolamine to P-choline increased in both membrane fractions at both temperatures. In plastids, the ratio of mono- to digalactosyldiacylglycerol declined substantially at 2C but not at 15C.