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Verification of somatic hybrids by expressed sequence tag–simple sequence repeat marker analysis. Selected EST-SSRs have proven to have double capabilities, simultaneously and unambiguously determining both the sources of donor genomes and the level of ploidy

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. Amplified fragments were visualized on an ABI Prism 3130 XL and scored semiautomatically with the GeneMapper 3.7 software (Applied Biosystems, Carlsbad, CA). Simple sequence repeat markers. The primer sequences for the SSR markers used in this study were

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. Most of the world's cultivars are selections from local wild vegetation. Based on simple sequence repeat markers, most cultivars have been assigned to one of four major geographical groups: Central European, Black Sea, English or Spanish

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. Fig. 1. Simple sequence repeat amplified with primer ID73 in 63 DNA of carpetgrass, Marker is 100-base pair DNA ladder, material number was showed in Table 1 . Statistical analysis. The coefficients of genetic similarity among the 63 carpetgrass

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. Simple sequence repeat (SSR) marker name and linkage group of the 78 SSRs used for peach related species identification. z PCR amplification. PCR amplification was performed in 20-µL reaction volumes containing 20 ng of genomic DNA, 10 µL of 2× Taq PCR

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additional four cultivars from the University of Georgia ( Table 1 ). Table 1. The origins of 58 Paspalum vaginatum accessions and four cultivars used in genetic diversity analysis revealed with simple sequence repeat markers. All materials were propagated

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for genic simple sequence repeat marker amplification and transferability in this study. DNA isolation. Genomic DNA for each cultivar was isolated from young leaves using the DNeasy Plant Mini Kit (Qiagen, Valencia, CA) according to the manufacturer

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this mixture was loaded on a 6.5% acrylamide gel using a LI-COR® Biosciences 4300 DNA Analyzer. Gel images were scored visually. Table 1. Simple sequence repeat markers developed from bermudagrass expressed sequence tags or genomic DNA for this study

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polymorphic loci (SAMPL) ( Tseng et al., 2002 ), and simple sequence repeat markers (SSRs) ( Gichuru et al., 2006 ). Microsatellites developed for genotyping sweetpotato ( Buteler et al., 1999 ; Hu et al., 2004 ) have been widely used for diversity analysis

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collected in Taiwan and appear to be the only H. paniculata germplasm in the United States that was not either introduced from Japan or bred from Japanese germplasm. Microsatellite, or simple-sequence repeat (SSR), markers provide a useful method for

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