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as random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), cleaved amplified polymorphic sequence (CAPS), and simple sequence repeat (SSR) ( Deng et al., 1992 ; Fu et al., 2004 ; Grosser et al., 1995 , 2004 ; Xu et

. Amplified fragments were visualized on an ABI Prism 3130 XL and scored semiautomatically with the GeneMapper 3.7 software (Applied Biosystems, Carlsbad, CA). Simple sequence repeat markers. The primer sequences for the SSR markers used in this study were

. Most of the world's cultivars are selections from local wild vegetation. Based on simple sequence repeat markers, most cultivars have been assigned to one of four major geographical groups: Central European, Black Sea, English or Spanish

Simple sequence repeat (SSR), also called microsatellites, are sections of DNA consisting of tandemly repeated mono-, di-, tri, tetra-, or pentanucleotide units that occur in abundance within the genomes of most eukaryotes ( Powell et al., 1996

; Morimoto et al., 1997 ), inter-simple sequence repeat markers ( Casasoli et al., 2001 ), restriction fragment length polymorphism markers ( Morimoto et al., 1997 ), and amplified fragment length polymorphism markers ( Yamamoto et al., 1998 ), have been used

. Simple sequence repeat (SSR) marker name and linkage group of the 78 SSRs used for peach related species identification. z PCR amplification. PCR amplification was performed in 20-µL reaction volumes containing 20 ng of genomic DNA, 10 µL of 2× Taq PCR

for genic simple sequence repeat marker amplification and transferability in this study. DNA isolation. Genomic DNA for each cultivar was isolated from young leaves using the DNeasy Plant Mini Kit (Qiagen, Valencia, CA) according to the manufacturer

carpetgrass accessions from China ( Table 1 ). Table 2. Amplification information of simple sequence repeat primers in carpetgrass accessions. Primer screening and DNA amplification. Fourteen primer pairs with clear gel bands, reproducible fragments, and

ultimately to the field. Table 1. Cross, parents, and simple sequence repeat (SSR) profiles of Prunus parents and hybrids tested in this study. z DNA extraction, simple sequence repeat primers, and polymerase chain reactions. Total genomic DNA was extracted

microsatellite primers for Paeonia suffruticosa Biol. Plant. 55 708 710 Kumar, S. Shah, N. Garg, V. Bhatia, S. 2014 Large scale in-silico identification and characterization of simple sequence repeats (SSRs) from denovo assembled transcriptome of Catharanthus