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Yaser Hassan Dewir, Abdulhakim A. Aldubai, Salah El-Hendawy, Abdullah A. Alsadon, Mayada Kadry Seliem, and Yougasphree Naidoo

used to propagate this species. The aim of this study was to develop in vitro propagation of C. erectus through axillary shoot proliferation. Materials and Methods Plant material, surface disinfection, and culture establishment. Shoot tips, 3–5 cm

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Xiuli Shen, William S. Castle, and Frederick G. Gmitter Jr

tissues from mature male trees. We have established a protocol for micropropagation of a Casuarina hybrid ( C. equisetifolia L. × C. glauca Sieber ex Spreng) using epicotyl explants excised from seeds germinated in vitro. Shoot proliferation was

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Choun-Sea Lin, Krishnan Kalpana, Wei-Chin Chang, and Na-Sheng Lin

shoots proliferation ( Kapoor and Rao, 2006 ; Nadgauda et al., 1990 ; Sood et al., 2002 ). However, it is very difficult to obtain bamboo reproductive tissues in the field. Lin and Chang (1998) used field-grown vegetative shoot meristems to induce

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Maria Luiza De Oliveira, James G. Thomson, and Ed Stover

juvenility. When a highly desirable rootstock or scion is introduced, the material available for propagation is often limited. Axillary shoot proliferation through tissue culture is an alternative propagation method that increases availability of material in

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Maurizio Micheli, Daniel Fernandes da Silva, Daniela Farinelli, Graziana Agate, Rafael Pio, and Franco Famiani

concentration of neem oil and the subculture on the multiplication rate and total number of leaves on the proliferated shoots. Table 3. Main effects of two concentrations of neem oil added to the medium on the proliferation of ‘Moraiolo’ uninodal explants in

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Fang Geng, Renae Moran, Michael Day, William Halteman, and Donglin Zhang

; Webster and Jones, 1989 ). Efficiency of proliferation requires a rapid increase in the number of new shoots that elongate sufficiently to transfer to the rooting stage. For some woody plant taxa or genotypes, in vitro shoot growth is stunted with poor

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Dennis P. Stimart, John C. Mather, and Kenneth R. Schroeder

Expanding shoot tips of Pulmonaria `Roy Davidson' and Pulmonaria saccharata `Margery Fish' were cultured in vitro on a modified Murashige and Skoog medium containing BA to establish proliferating cultures for use in comparing BA concentrations on shoot proliferation and rooting. The optimum level for shoot proliferation was 8.8 μm BA. Greatest rooting was on medium without BA. Genotype and time in culture influenced shoot and root counts. Chemical names used: N6-benzyladenine (BA)

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Fang Geng, Renae Moran, Michael Day, William Halteman, and Donglin Zhang

Micropropagation can rapidly increase numbers of stock plants, particularly for new cultivars that are available in limited quantities. Because of their genetic stability and potential for high proliferation, single- and multiple-node shoot pieces

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Karim H. Al-Juboory

Shoot proliferation of Gardenia jasminoides was achieved from cultured shoot tips on Nitsch and Nitsch medium supplemented with different levels (0.0–0.6 mg·L–1) of zeatin, BAP, BA, TDZ, and kinetin. Zeatin proved to be the most effective cytokinin for stimulating shoot proliferation. Shoot length obtained with zeatin was shorter than with other cytokinins and shoot leaves were narrower. Shoot tips were cultured on Nitsch and Nitsch medium supplemented with BA at 4.0 mg·L–1 combined with IAA at 0.0–0.2 mg·L–1. The results indicated that BA at 4.0 mg·L–1 with 0.1 IAA produced greater shoot proliferation. Plantlets regenerated in vitro were then transferred to a mixture of 1 peat: 1 perlite: 1 soil and acclimatized for potting. Our results show that micropropagation of Gardenia has high potential for use in commercial industry.

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F.A. Hammerschlag, R.H. Zimmerman, and A.C. Smigocki

`McIntosh' apple shoots were inoculated in vitro with Agrobacterium tumefaciens strain tms328::Tn5 (tms) carrying a functional cytokinin gene. Callus tissue, removed from the infected stems, produced shoots on shoot proliferation medium. After three subcultures, axillary shoot production from a tms-infected putative transformant was eight times that of controls. Subsequent shoot production on three different levels of BA (3, 6 and 10 uM) was significantly greater than from controls on all levels of BA. PCR analysis of putative transformants revealed an expected 503 bp DNA fragment corresponding to the amplified portion of the cytokinin gene. After 6 months of in vitro propagation, proliferation rates of shoots obtained from the original transformants were similar to the controls and the expected PCR fragment of 503 bp could only be detected by Southern analysis. Even though the T-DNA appears to be lost from the apple genome, the data suggest that the tms strain may be useful in co-infection experiments to induce shoot formation, thus avoiding difficult regeneration procedures.