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P. Martínez-Gómez, M. Rubio, and F. Dicenta

The resistance to a Dideron isolate of Plum pox virus, which causes sharka disease, of four apricot (Prunus armeniaca L.) cultivars from North America (`Harlayne', `Henderson', `Sunglo', and `Veecot') and a Greek cultivar Lito (a cross of American cultivar Stark Early Orange × Greek cultivar Precoce Tirynthos) was evaluated. `Stark Early Orange' and `Canino', previously rated as resistant and susceptible respectively, were included as controls. Resistance, herein, was defined as inability to infect plants by graft-inoculation and negative assays by enzyme-linked immunosorbent assay. Cultivars found to be resistant were: `Harlayne', `Henderson', `Sunglo', `Lito', and `Stark Early Orange'. Cultivars Veecot and Canino were susceptible.

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P. Martínez-Gómez, M. Rubio, F. Dicenta, and T.M. Gradziel

Sharka [(plum pox virus (PPV)] mainly affects Prunus species, including apricot (Prunus armeniaca L.), peach (Prunus persica L.), plum (Prunus salicina Lindl., Prunus domestica L.), and, to a lesser degree, sweet (Prunus avium L.) and sour cherry (Prunus cerasus L.). Level of resistance to a Dideron isolate of PPV in seven California almond [P. dulcis (Miller) D.A. Webb], five processing peach cultivars, and two peach rootstocks was evaluated. In addition, almond and peach selections resulting from interspecific almond × peach hybridization and subsequent gene introgression were tested. Evaluations were conducted in controlled facilities after grafting the test genotypes onto inoculated GF305 peach rootstocks. Leaves were evaluated for PPV symptoms during three consecutive cycles of growth. ELISA-DASI and RT-PCR analysis were also employed to verify the presence or absence of PPV. Peach cultivars and rootstocks showed sharka symptoms and were ELISA-DASI or RT-PCR positive for some growth cycles, indicating their susceptibility to PPV. Almond cultivars and almond × peach hybrids did not show symptoms and were ELISA-DASI and RT-PCR negative, demonstrating resistance to PPV. Two (almond × peach) F2 selections as well as two of three backcrossed peach selections also showed a resistant behavior against the PPV-D isolate. Results demonstrate a high level of resistance in almond and indicate potential for PPV resistance transfer to commercial peach cultivars.

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Jose Martínez-Calvo, Gerardo Llácer, and Marisa Luisa Badenes

‘Rafel’ and ‘Belgida’ are mid- to early-ripening apricot cultivars ( Prunus armeniaca L.) with good yield, excellent fruit quality, self-compatibility, and resistance to Sharka, a disease caused by the Plum pox virus , a serious limiting factor

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Maria L. Badenes, Jose Martínez-Calvo, Helena Gómez, and Elena Zuriaga

). The cultivar Goldrich is self-incompatible, with genotype S 1 S 2 ( Vilanova et al., 2005 ), which prevents self-pollination and cross-pollination from genotypes with the same alleles and provides resistance to sharka ( Moustafa et al., 2001 ). It is

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Jose Martínez-Calvo, Gerardo Llácer, and Maria Luisa Badenes

‘Moixent’ is a self-compatible, early-ripening apricot cultivar ( Prunus armeniaca L.) with excellent fruit quality and resistance to sharka (plum pox virus), a serious disease limiting apricot fruit production in affected areas. ‘Moixent’ fruits

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José Egea, Manuel Rubio, José A. Campoy, Federico Dicenta, Encarna Ortega, María D. Nortes, Pedro Martínez-Gómez, Antonio Molina, Antonio Molina Jr, and David Ruiz

also characterized by their adaptation to climatic conditions in the southeast of Spain and their resistance to sharka [ Plum pox virus (PPV)], a serious limiting factor for apricot fruit production in affected areas ( Kölber, 2001 ). ‘Mirlo Blanco

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David Ruiz, Manuel Rubio, Pedro Martínez-Gómez, Jesús López-Alcolea, Federico Dicenta, Encarna Ortega, María Dolores Nortes, Antonio Molina, Antonio Molina Jr., and Jose Egea

Spain). Plum pox virus resistance. All apricot cultivars traditionally grown in Europe are susceptible to sharka disease caused by PPV, whereas several cultivars from North America such as ‘Goldrich’, ‘Sunglo’, ‘Orange Red’, and ‘Stark Early Orange

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Bayram Murat Asma

.58 ± 3.9 mm in length and 1.66 ± 0.2 mm width when sampled in Aug. 2011. Plum pox virus resistance Sharka, caused by PPV, is an important disease for plum, apricot, cherry, and peach as well as other Prunus species. It is known that the apricot

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José Egea, Jose A. Campoy, Federico Dicenta, Lorenzo Burgos, Jose L. Patiño, and David Ruiz

cultivars. In addition, they have a reduced need for thinning, which reduces the cost of cultivation. Plum pox virus resistance. Sharka disease, caused by the Plum pox virus (PPV), is a serious limiting factor for temperate fruit production in those areas

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Marek Szymajda and Edward Żurawicz

reaching harvest maturity. The tree grows moderately vigorously and comes early into bearing fruit. ‘Kalipso’ is tolerant to Plum pox virus (PPV), a causal agent of sharka (plum pox) disease. With standard orchard protection, the flowers, shoots, and