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Rafel Socias i Company, Àngel Fernández i Martí, Ossama Kodad, and José M. Alonso

Although self-compatibility was discovered in almond as early as 1945 ( Almeida, 1945 ), no attention was paid to the issue until the 1970s. The importance of self-compatibility in almond-growing and in breeding for new self-compatible cultivars

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Reut Niska, Martin Goldway, and Doron Schneider

, 2000 ). However, some loquat cultivars such as ‘Akko 1’, ‘Mogi’, ‘Pale Yellow’, ‘Advance’, and ‘Tanaka’ are self-fertile or partially self-fertile ( Cuevas et al., 2003 ; Morton, 1987 ; Tous and Ferguson, 1996 ). In the Maloideae subfamily, self-compatibility

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L. Burgos, T. Berenguer, and J. Egea

Eight apricot (Prunus armeniaca L.) cultivars were self- and cross-pollinated to determine pollen compatibility. Pollen tube growth in the laboratory and the percentage of fruit set in the orchard were evaluated. The results confirmed that `Moniqui Fino' and `Velázquez Tardío' are self-incompatible and established that `Gitano', `Pepito del Cura', and `Velázquez Fino' are also self-incompatible. No cross-incompatibility was found in the 25 cross-combinations.

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Ossama Kodad, Rafel Socias i Company, Ana Sánchez, and M. Margarida Oliveira

. The pistils were autoclaved in a 5% solution of Na 2 SO 3 for 12 min at 1.2 kg·cm −2 . Self- and cross-compatibility was assessed by pollen tube growth after observation in a Leitz Ortholux II microscope (Leitz, Wetzlar, Germany) with ultraviolet

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Chitose Honsho, Masami Kotsubo, Yuri Fukuda, Yosui Hamabata, Yoshikazu Kurogi, Aya Nishiwaki, and Takuya Tetsumura

pollination in this study. Discussion Self-compatibility in ‘Nishiuchi Konatsu’. Hyuganatsu cannot produce fruits by self-pollination as a result of its self-incompatible nature ( Miwa, 1951 ). On the other hand, ‘Nishiuchi Konatsu’, a bud mutation of

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Ryutaro Tao, Tsuyoshi Habu, Hisayo Yamane, Akira Sugiura, and Kazuya Iwamoto

Self-compatible cultivars of Japanese apricot (Prunus mume Sieb. et Zucc.) have a horticultural advantage over self-incompatible ones because no pollinizer is required. Self-incompatibility is gametophytic, as in other Prunus species. We searched for molecular markers to identify self-compatible cultivars based on the information about S-ribonucleases (S-RNases) of other Prunus species. Total DNA isolated from five self-incompatible and six self-compatible cultivars were PCR-amplified by oligonucleotide primers designed from conserved regions of Prunus S-RNases. Self-compatible cultivars exhibited a common band of ≈1.5 kbp. Self-compatible cultivars also showed a common band of ≈12.1 kbp when genomic DNA digested with HindIII was probed with the cDNA encoding S 2-RNase of sweet cherry (Prunus avium L.). These results suggest that self-compatible cultivars of Japanese apricot have a common S-RNase allele that can be used as a molecular marker for self-compatibility.

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Sandra M. Reed

Clethra alnifolia, which is commonly known as summersweet, is an attractive deciduous shrub that produces fragrant flower in mid-summer. Breeding efforts are hampered by a lack of information on the reproductive behavior of this native species. The objective of this study was to evaluate self-compatibility in C. alnifolia. Pollen germination and pollen tube growth in styles were examined following self- and cross-pollinations using fluorescence microscopy. Seed set and germination were compared following self- and cross-pollinations. While self-pollen tubes appeared to grow slightly slower than cross-pollen tubes, there was no indication of a self-incompatibility system acting at the stigmatic or stylar level in C. alnifolia. Self-pollinations of `Hummingbird' and `Ruby Spice' produced fewer seeds than did cross-pollinations of these cultivars. Germination of all seed obtained from this study was too poor to allow a comparison of germination rates of the self- and cross-pollinated seed. However, because a few self-progeny were obtained, emasculation is recommended when making controlled pollinations. The presence of a late-acting self-incompatibility system or early acting inbreeding depression was proposed as being responsible for the lower seed set following self-pollination.

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Santiago Vilanova, Carlos Romero, Gerardo Llácer, María Luisa Badenes, and Lorenzo Burgos

This report shows the PCR-based identification of the eight known self-(in)compatibility alleles (S 1 to S 7 and S c) of apricot (Prunus armeniaca L.). Two sets of consensus primers, designed from P. armeniaca S-RNase genomic sequences and sweet cherry (P. avium L.) S-RNase-cDNAs, were used to amplify fragments containing the first and the second S-RNase intron, respectively. When the results obtained from the two PCRs were combined, all S-alleles could be distinguished. The identity of the amplified S-alleles was verified by sequencing the first intron and 135 base pairs (bp) of the second exon. The deduced amino acid sequences of these fragments showed the presence of the C1 and C2 Prunus L. S-RNase conserved regions. These results allowed us to confirm S-genotypes previously assigned by stylar ribonuclease analyses and to propose one self-(in)compatibility group (I) and one universal donor group (O) containing unique S-genotypes and self-compatible cultivars (SC). This PCR-based typing system also facilitates the identification of the S c-allele and might be a very useful tool for predicting self-compatibility in apricot breeding progenies.

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Daniel J. Bell, Lisa J. Rowland, John Stommel, and Frank A. Drummond

optimization, have increased harvest yields in Maine by a factor of four during the last two decades ( Yarborough, 2004 , 2009a ). Despite these gains, there remains much to be learned regarding the specific genetic basis of cross-compatibility factors

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Akiko Watari, Toshio Hanada, Hisayo Yamane, Tomoya Esumi, Ryutaro Tao, Hideaki Yaegaki, Masami Yamaguchi, Kenji Beppu, and Ikuo Kataoka

., 2003 ; Yamane et al., 2003c ), and apricot ( Romero et al., 2004 ). The results from these studies have led to the development of not only molecular typing methods for S -haplotypes ( Tao et al., 1999 ), but also molecular markers for self-compatibility