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Abstract

A successful host-parasite relationship was established in the stems of both resistant and susceptible snap beans (Phaseolus vulgaris L.) with the root-knot nematode Meloidogyne incognita. Thirty days after beans were planted in infested soil, all had stem galls containing egg-laying females. Histological studies showed giant cells developed from both vascular and cortical tissues.

Open Access

Abstract

Experiments were conducted to evaluate the effect of fresh and aged conifer barks on galling by the root-knot nematode [Meloidogyne incognita (Kofoid and White) (Chitwood)] on tomato (Lycopersicon esculentum Mill.) roots. Fresh bark (stored at sawmill) exhibited significant nematicidal activity (reduced galling) when used as a medium component [50% or 75% with sand (v/v)]. Galling on tomatoes grown in aged bark (used as a culturing medium for tomatoes for 5 years) was extensive. When 10% or 20% fresh conifer bark was mixed into beds, galling was less extensive on tomato roots than on roots from tomatoes grown in an unamended medium. The nematicidal property of conifer bark diminished during long-term use. Increases in medium pH, which occurred during continuous cropping, could have contributed to the reduced nematicidal activity with time.

Open Access

was ended after 114 d (14 July 2006) and the following data were collected: number of egg masses per root system, number of eggs per root system, number of root galls per root system, and dry root weight (dried at 70 °C in aluminum foil until no more

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15% solution of McCormick's red food color ( Thies et al., 2002 ) for 15 to 20 min to stain the egg masses. The root systems were carefully rinsed under running tap water and evaluated for galling severity and egg mass production using a 1 to 9 scale

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‘Charleston Belle’ and ‘Carolina Wonder’ exhibited partial loss of resistance when grown at 32 °C for 8 weeks after inoculation with M. incognita , these cultivars had root gall severity indices that were still in the low resistance range, and the number of

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germplasm by determining the incidence of galls and egg masses following standardized inoculation of plants in greenhouse evaluations ( Cervantes-Flores et al., 2002 ). While RKN effects on storage root yield have been demonstrated in field plots ( Dukes et

Open Access

quality traits including total soluble solids (Brix) were recorded on 30 July, 3 Aug., 6 Aug., 11 Aug., 13 Aug., and 17 Aug. 2009. At the end of the harvest season on 26 Aug., roots of all plants were dug and evaluated for percent of root system galled and

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pest and disease pressure. One of the major pest management challenges is RKNs ( Meloidogyne spp.), which thrive in warm weather and moist, sandy soils ( Roberts et al., 2005 ; Sasser, 1980 ). RKNs cause root galls that damage the root system and

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ended 60 d after inoculation and the following data were collected for inoculated seedlings: number of egg masses per root system, number of eggs per root system, and fresh root weight. Root systems were also rated for the percentage of root galls

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using the 0- to 5-point gall index, with 0 = immune, 1 = highly resistant, 2 = resistant, 3 = moderately resistant, 4 = moderately susceptible to susceptible, and 5 = highly susceptible ( Taylor and Sasser, 1978 ). Root fresh biomass (measured in grams

Open Access