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J. Kevin Parris, Thomas G. Ranney, Halina T. Knap, and W. Vance Baird

genome size using DAPI (AT preferential) or propidium iodide (PI) (intercalating) fluorochrome stains and estimate bp composition for representative taxa from 10 taxonomic sections. Materials and Methods Relative genome size and ploidy level determination

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Kimberly Shearer and Thomas G. Ranney

dogwoods, sampling for genome size and ploidy level of species, cultivars, and hybrids has been limited and little is known concerning ploidy level and genome size of specific cultivars and hybrids. The objectives of this study were to determine relative

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Thomas G. Ranney, Tracy H. Thomasson, Kristin Neill, Nathan P. Lynch, and Mark Weathington

ploidy and relative genome size for a diverse collection of species and cultivars of aucuba using flow cytometry and cytology and to make additional observations on the heritability of spotted leaf variegation of specific cultivars. Materials and Methods

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Joseph J. Rothleutner, Mara W. Friddle, and Ryan N. Contreras

relative genome sizes and produce ploidy estimates across a wide selection of Cotoneaster including its breadth of taxonomic groups. Materials and Methods Plant material. Germplasm was collected through various means including whole plants from nurseries

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David J. Roberts and Dennis J. Werner

. Stained nuclei suspensions were refrigerated at 4 °C for 1 h before being analyzed via flow cytometry. All samples were completely randomized before analysis. Flow cytometry. Holoploid genome size estimates were determined by measuring the relative

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Dominic A. Gillooly and Thomas G. Ranney

available for analysis. Relative 2C genome sizes were determined using flow cytometry on recently expanded leaves ( Greilhuber et al., 2007 ). Sample tissue was combined with an internal standard ( Pisum sativum L. ‘Ctirad’, 2C DNA content = 8.76 pg

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Thomas G. Ranney, Connor F. Ryan, Lauren E. Deans, and Nathan P. Lynch

recombination and illegitimate recombination ( Grover and Wendel, 2010 ; Soltis et al., 2015 ). There have only been limited reports on chromosome numbers and relative genome sizes for species and cultivars of Illicium . A base chromosome number of x = 14

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Alan T. Whittemore and Zheng-Lian Xia

additional trees with known chromosome numbers will give us a firmer understanding of variation in genome size. In addition, a broad survey of nuclear DNA content in Ulmus and related genera using flow cytometry could provide more information on the

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Whitney D. Phillips, Thomas G. Ranney, Darren H. Touchell, and Thomas A. Eaker

]} ( Table 1 ). Six additional interspecific hybrids were also interspersed with the triploids. Plants were completely randomized. Table 1. Ploidy, relative holoploid genome size, female reproductive characteristics, and reproductive pathways for selected

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Todd J. Rounsaville, Darren H. Touchell, and Thomas G. Ranney

most significant reduction in fertility was observed with H2008-091-004, which had a relative fertility of 0.7% when compared with the diploid control. Reproductive pathways. Genome sizes (2C) recorded from 568 progeny of open-pollinated diploid and