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considerations Genes Immun. 6 279 284 Iskandar, H.M. Simpson, R.S. Casu, R.E. Bonnett, G.D. Maclean, D.J. Manners, J.M. 2004 Comparison of reference genes for quantitative real-time polymerase chain reaction analysis of gene expression in sugarcane Plant Mol
Selection of suitable reference genes for quantitative Real-time polymerase chain reaction in Prunus mume during flowering stages and under different abiotic stress conditions J. Amer. Soc. Hort. Sci. 139 113 122 doi: 10.1051/fruits/2014004 10.21273/JASHS
Zea mays and Spinacia oleracea Plant Mol. Biol. Rpt. 30 478 487 10.1007/s11105-011-0354-x Chen, X. Truksa, M. Shah, S. Weselake, R.J. 2010 A survey of quantitative real-time polymerase chain reaction internal reference genes for expression studies in
in a population created by crossing broccoli and cabbage, only 2% of the F 2 plants produced cabbage-like heads and none of these were of commercial grade. The development of a linkage map specific to broccoli using polymerase chain reaction (PCR
recovery of 99.2% with a cv of 20.2% for the seven 1-mg samples of mycelium. Quantitative real-time polymerase chain reaction assay of inoculated onion bulb disks. The ability of the real-time PCR assay to detect and quantify B. aclada mycelium
Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) is now widely used in molecular experiments in diverse fields, including molecular medicine, biotechnology, microbiology, and human clinical diagnostics ( Bustin and Dorudi, 1998
used to design primers for the semiquantitative reverse transcription–polymerase chain reaction and quantitative reverse transcription–polymerase chain reactions using Primer 3 ( Rozen and Skaletsky, 1998 ) and Primer 3Plus software ( Untergasser et al
)-mass spectrometry (MS), characterize the MybA-related genes at the color locus via capillary electrophoresis and quantitative real-time polymerase chain reaction (qRT-PCR) in Kyoho and its derivatives to identify the germplasm resources with higher total anthocyanin
. RNA extraction and quantitative reverse transcription polymerase chain reaction. Leaves at 3, 6, 12, 18, and 24 h, and 3 and 5 d after inoculation, were ground with liquid nitrogen, total RNA was extracted using TRIzol reagent (RNAiso Plus; Takara
/g FW. Three samples were drawn from each replication and measurements were taken for each sample. RNA preparation, RT-PCR, and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Total RNA was isolated from cells using the CTAB method