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Arthur Villordon and Jason Franklin

Shape measurements in horticultural research have generally been expressed as ratios or indexes. Computer-based image analysis enables the objective quantification and statistical analysis of two-dimensional sample shape variability. In addition, the availability of public domain software facilitates the inexpensive but accurate quantification of object shape in horticultural research. We describe the procedures for measuring sample shape using the following publicly available software: ImageJ, ImageTool, and SHAPE. Using U.S. #1 sweetpotato storage root samples from plots subjected to various weed control treatments, we detected significant differences in elongation, compactness, as well as shape attributes. We also measured size and shape variability from representative fruit, leaf, and floral organ samples. The results demonstrate that, where possible, measurement of two-dimensional samples can be undertaken inexpensively and accurately using public domain software applications.

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Arthur Villordon, Wambui Njuguna, Simon Gichuki, Philip Ndolo, Heneriko Kulembeka, Simon Jeremiah, Don LaBonte, Bernard Yada, Phinehas Tukamuhabwa, and Robert Mwanga

Web-based information delivers real-time or near-real-time data to clientele and other stakeholders. Although proprietary methods are available for interactively searching and updating databases through web interfaces, these methods generally require varying costs to maintain licensing agreements. The availability of publicly available software that require minimal or flexible licensing costs provide a cost-effective alternative to institutions that are considering access to databases via a web-accessible interface. For example, if a current web server is already configured to support hypertext preprocessor (PHP) scripts and MySQL databases, all that needs to be installed is a form script to allow the searching, inserting, and deleting of records. We describe procedures, software, and other applications that we used to develop a publicly accessible web interface to an experimental database of representative sweetpotato accessions in Kenya. The web address of this database is This site also contains links to sweetpotato collection sites in Kenya, Tanzania, and Uganda graphically shown using a public domain GIS viewer. This demonstrates that public domain web-based tools can be configured not only to support collaborative activities among researchers in various locations, but also to provide relevant data to clients and other stakeholders.

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Bin Cai, Cheng-Hui Li, Ai-Sheng Xiong, Ri-He Peng, Jun Zhou, Feng Gao, Zhen Zhang, and Quan-Hong Yao

DNA-binding domains ( Riaño-Pachón et al., 2007 ; Riechmann et al., 2000 ). For some families, a TF contains a single domain, which is sufficient to assign its membership. However, in other families, a TF may contain more than one DNA-binding domain

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A. Villordon, S. Gichuki, H. Kulembeka, S.C. Jeremiah, and D. Labonte

One of the secondary centers of genetic diversity for the sweetpotato [Ipomoea batatas (L.) Lam.] is located in Africa. We have developed a geo-referenced database of sweetpotato accessions for Tanzania and Kenya that is accessible by stakeholders and other users. Public domain base maps and other files were used to generate the underlying GIS components. DIVA-GIS was used to convert existing spreadsheet-based accession and passport data into GIS-compliant files. ALOV Map, a public domain Java application for publishing vector and raster maps, was used to provide the framework for a web-accessible GIS database. This demonstrates that the availability of publicly available software requiring minimal or flexible licensing costs provide a cost-effective alternative to institutions that are considering developing GIS databases as well as enabling web accessibility to such resources. DIVA-GIS was also used to predict potential distribution of sweetpotato germplasm in Sub-Saharan Africa using the built-in ecological niche modelling tool. We describe procedures, software, and other applications that we used to develop a publicly accessible web interface to a GIS database of sweetpotato germplasm collections in Kenya and Tanzania.

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Gary W. Stutte and Elizabeth C. Stryjewski

Manual methods for estimating root length are tedious and time-consuming. Image capture and analysis systems can be used to obtain precise measurements of root length and growth angle. Root activity can also be determined through analysis of the mean pixel intensity of a digitized image. Both commercial (the IBM-compatible ICAS System) and public domain (the Macintosh-based NIH Image) image capture and analysis software have been used to analyze intact root systems. Examples of ICAS classification of hydroponic and soil-grown root systems will be presented. Advantages of the NIH Image software for analysis of micro-gravity experiments aboard the Space Shuttle will be discussed.

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Min Hyeong Kwon, Jongyun Kim, Changwan Seo, Chiwon W. Lee, Eu Jean Jang, and Woo-Kyun Lee

, awareness of the importance of environmental education increased, and public institutions began providing more spaces for children to experience the natural environment ( Eberbach, 1988 ; Halverson et al., 2008 ). Against this backdrop, natural and

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Sabine R. Green and Geno A. Picchioni

of the course together for a real-world experience is a challenge for the instructor. Engaging a student in a psychomotor task to reinforce what was learned in part of experiential learning is known as cognitive domain ( Newcomb et al., 2004

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Derek W. Barchenger, Joseph I. Said, Yang Zhang, Mingzhou Song, Franchesca A. Ortega, Yeasong Ha, Byoung-Cheorl Kang, and Paul W. Bosland

, and pharmaceuticals. Recently, complete chloroplast, mitochondrial, and nuclear genome sequences of chile pepper were made available publicly ( Jo et al., 2014 ; Kim et al., 2014 ; Park et al., 2016 ; Qin et al., 2014 ; Raveendar et al., 2015a

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A.L. McFarland, T.M. Waliczek, and J.M. Zajicek

comments or suggestions regarding the campus environment. Quality of life of university students instrumentation. The instrument selected to measure quality of life of students consisted of two separate domains: an affective domain ( Roberts and Clifton

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Ebrahiem Babiker, Stephen J. Stringer, Hamidou F. Sakhanokho, John J. Adamczyk Jr., and Arlen D. Draper

as alternative to conventional landscapes. ‘Muffin Man’ is a new public-domain, edible ornamental rabbiteye blueberry cultivar developed by the U.S. Department of Agriculture (UDSA), Agricultural Research Service (ARS) Thad Cochran Southern