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David M. Czarnecki II, Amanda J. Hershberger, Carol D. Robacker, David G. Clark, and Zhanao Deng

pollen that can be transferred onto native lantana’s flowers by pollinators. Dehgan (2006) indicated the existence of a wide range of pollen stainability (from less than 5% in Patriot™ ‘Sunburst’ to more than 80% in ‘Professor Raoux’) in L . camara

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M. L. Weaver, H. Timm, M. J. Silbernagel, and D. W. Burke


Viability of pollen grains of isogenic sibling bean (Phaseolus vulgaris L.) selections of known tolerance of sensitivity to high temperatures (HT), as previously determined by pod retention and seed yield, was compared to that of a common parent bean selection and a cowpea [Vigna unguiculata (L.) Walp.] cultivar. Exposure of newly opened flowers to temperatures of 35° or 41°C reduced the viability of pollen grains in all bean selections. Pollen of all sibling selections was less affected by HT than pollen of their common parent suggesting transgressive segregation of factors for HT tolerance. At 41°, most pollen grains were destroyed in the parent bean selection and the 2 HT-sensitive siblings, whereas 44% to 55% of the pollen grains appeared to be viable in the 2 HT-tolerant siblings. Pollen viability of the HT-tolerant cowpea cultivar was not reduced by temperatures to 41°. Pollen staining indicated an interrelationship between pollen viability and tolerance to HT stress among the bean selections. The technique described has the potential for rapid selection of HT-tolerant genotypes in hybrid populations.

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Katsumi Suzuki, Tadashi Tsukaguchi, Hiroyuki Takeda, and Yoshinobu Egawa

Pod yield of `Kentucky Wonder' green bean (Phaseolus vulgaris L.) decreased at high temperatures due to a reduction of pod set. A highly positive correlation was observed between pod set and pollen stainability in flowers that were affected by heat stress about 10 days before anthesis. Pollen stainability was decreased by heat stress applied 8 to 11 days before flowering under controlled environment conditions. When mean air temperature during this period exceeded 28 °C, pollen stainability decreased under field conditions. Low pollen stainability indicated sensitivity to high temperatures about 10 days before flowering. A heat-tolerant cultivar showed higher pollen stainability than did heat-sensitive cultivars under high temperatures. These results demonstrated that heat tolerance at an early reproductive stage could be evaluated by analyzing pollen stainability using flowers developed under high temperatures.

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Zhiyong Hu, Min Zhang, Qigen Wen, Jie Wei, Hualin Yi, Xiuxin Deng, and Xianghua Xu

flower buds collected from three trees before anthesis were sampled, all the pollens were freshly harvested. For pollen stain viability, one anther per flower bud was collected from six flower buds, mixed, and spread on 10 slides, and then three

Open access

Shih-wen Lin, Tsung-han Lin, Cynthia Kung Man Yee, Joyce Chen, Yen-wei Wang, Manoj Kumar Nalla, and Derek W. Barchenger

requires measuring fruit set over an extended time and is sensitive to variations in temperature that could permit an otherwise heat-sensitive plant to set fruit. Pollen viability is usually monitored by staining the harvested pollen and counting pollen

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Keri D. Jones, Sandra M. Reed, and Timothy A. Rinehart

examined. Finally, fertility of triploid cultivars was estimated through pollen staining and controlled pollinations. Materials and Methods Plant materials. Cuttings were taken in Summer 2005 from a single plant each of 75 H. macrophylla

Open access

Renjuan Qian, S. Brooks Parrish, Sandra B. Wilson, Gary W. Knox, and Zhanao Deng

stainability on these porterweeds, which would be useful data in the breeding of noninvasive plants. Pollen staining has become a reliable method of determining pollen viability in hybridization studies ( Czarnecki et al., 2014 ). Other porterweed studies have

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Clara E. Trueblood, Thomas G. Ranney, Nathan P. Lynch, Joseph C. Neal, and Richard T. Olsen

collected from flowers at anthesis for each plant and used for viability tests. Pollen grains were stained in 40 μL of 1% acetocarmine on a microscope slide. The slide was heated three times over a hot plate set at medium–low temperature to heat without

Open access

Hsiang-I Lee and Michael J. Havey

pollen stainability in onion families possessing S cytoplasm and segregating for the dominant allele at Ms from three different sources. We observed that male fertility restoration conditioned by one source of the dominant Ms allele was incomplete and

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Pablo Bolaños-Villegas, Shih-Wen Chin, and Fure-Chyi Chen

standard for comparison of pollen division patterns. Table 1. Genome constitution and cytological characteristics of nine Doritaenopsis hybrids. Pollen staining with fluorescein diacetate (FDA). Pollen viability analyzed by staining