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David M. Czarnecki II, Amanda J. Hershberger, Carol D. Robacker, David G. Clark, and Zhanao Deng

pollen that can be transferred onto native lantana’s flowers by pollinators. Dehgan (2006) indicated the existence of a wide range of pollen stainability (from less than 5% in Patriot™ ‘Sunburst’ to more than 80% in ‘Professor Raoux’) in L . camara

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Katsumi Suzuki, Tadashi Tsukaguchi, Hiroyuki Takeda, and Yoshinobu Egawa

Pod yield of `Kentucky Wonder' green bean (Phaseolus vulgaris L.) decreased at high temperatures due to a reduction of pod set. A highly positive correlation was observed between pod set and pollen stainability in flowers that were affected by heat stress about 10 days before anthesis. Pollen stainability was decreased by heat stress applied 8 to 11 days before flowering under controlled environment conditions. When mean air temperature during this period exceeded 28 °C, pollen stainability decreased under field conditions. Low pollen stainability indicated sensitivity to high temperatures about 10 days before flowering. A heat-tolerant cultivar showed higher pollen stainability than did heat-sensitive cultivars under high temperatures. These results demonstrated that heat tolerance at an early reproductive stage could be evaluated by analyzing pollen stainability using flowers developed under high temperatures.

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Zhiyong Hu, Min Zhang, Qigen Wen, Jie Wei, Hualin Yi, Xiuxin Deng, and Xianghua Xu

flower buds collected from three trees before anthesis were sampled, all the pollens were freshly harvested. For pollen stain viability, one anther per flower bud was collected from six flower buds, mixed, and spread on 10 slides, and then three

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Keri D. Jones, Sandra M. Reed, and Timothy A. Rinehart

examined. Finally, fertility of triploid cultivars was estimated through pollen staining and controlled pollinations. Materials and Methods Plant materials. Cuttings were taken in Summer 2005 from a single plant each of 75 H. macrophylla

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Renjuan Qian, S. Brooks Parrish, Sandra B. Wilson, Gary W. Knox, and Zhanao Deng

stainability on these porterweeds, which would be useful data in the breeding of noninvasive plants. Pollen staining has become a reliable method of determining pollen viability in hybridization studies ( Czarnecki et al., 2014 ). Other porterweed studies have

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Clara E. Trueblood, Thomas G. Ranney, Nathan P. Lynch, Joseph C. Neal, and Richard T. Olsen

collected from flowers at anthesis for each plant and used for viability tests. Pollen grains were stained in 40 μL of 1% acetocarmine on a microscope slide. The slide was heated three times over a hot plate set at medium–low temperature to heat without

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Hsiang-I Lee and Michael J. Havey

pollen stainability in onion families possessing S cytoplasm and segregating for the dominant allele at Ms from three different sources. We observed that male fertility restoration conditioned by one source of the dominant Ms allele was incomplete and

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Pablo Bolaños-Villegas, Shih-Wen Chin, and Fure-Chyi Chen

standard for comparison of pollen division patterns. Table 1. Genome constitution and cytological characteristics of nine Doritaenopsis hybrids. Pollen staining with fluorescein diacetate (FDA). Pollen viability analyzed by staining

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Hazel Y. Wetzstein, Nadav Ravid, Erik Wilkins, and Adriana Pinheiro Martinelli

continue down the length of the style ( Fig. 6E ). Aniline blue staining of tissues observed under ultraviolet light shows the path of pollen tubes throughout much of the stigma ( Fig. 6D ) but which become directed to grow only within the central stylar

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Joshua H. Kardos, Carol D. Robacker, Michael A. Dirr, and Timothy A. Rinehart

. angustipetala . Pollen viability. Pollen viability was assessed using a fluorescein diacetate (FDA) staining procedure developed by Heslop-Harrison and Heslop-Harrison (1970) . Flowers were collected on the day of anthesis from five randomly chosen hybrids per