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germination rate and pollen amount have been reported in many studies ( Jiang et al., 2012 ; Ou et al., 2010 ; Stern and Gazit 1998 ; Xiang et al., 2000 ); however, systematic studies on the change of pollen germination rate and pollen amount from variety

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germination, pollen tube growth, fertilization and fruit set, and optimum timing for application ( Fallahi and Willemsen, 2002 ; Greene, 2002 ). Several reports have documented that temperature is the most important factor affecting pollen germination and

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. Masculinized genotypes Wife and Cherry Wine had pollen germination rates similar to those of genetically male Kentucky Sunshine ( Table 1 ). Wife had the greatest pollen germination percent compared with the other four masculinized female genotypes. Plants

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·L −1 calcium nitrate, and 200 g·L −1 sucrose ( Carreño et al., 2009 ). Pollen germination rate varied strongly by cultivar and less so by cluster position ( Table 2 ). Germination rates ranged from 2.5% to 18.2% in the hermaphroditic cultivars, and no

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, Tokyo). The pollen germination rate was calculated by randomly selecting no fewer than 600 pollen grains. Pollen germination rate (%) = pollen germination number of each field vision/total pollen number of each field × 100%. Images were taken using a

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periods were mostly the same for the four cultivars ( Supplemental Fig. 1 ). The pollen germination rates of HS, HJ, HX, and XL were 82.95%, 72.23%, 87.47%, and 81.60%, respectively ( Fig. 2 , Supplemental Fig. 2 ), indicating that they have good pollen

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). Table 1. Jujube cultivar pollen germination rates from 2012 to 2014 at Alcalde, NM. The last nine cultivars had only 1 year of observation, for reference only. For pollen germination tests, 0.35–0.4 mL of media was used per slide and smeared with a glass

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. Three plates were used for each treatment. The germination rates were assessed using a light microscope (Digital Fine Scope VC3500; Omron, Kyoto, Japan). The germination rate was determined from ≈100 pollen grains in each of three areas per plate. Spray

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anthers did not produce pollen. Therefore, their vitality was 0. Table 2. Pollen vitality and germination rates of Camellia oleifera . In vitro germination was used to detect the pollen germination rate ( Table 2 ). The in vitro germination rate

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rate, pollen germination ability, and changes in plant traits. Materials and Methods Propagating and managing plant materials. All parent plants for the test were planted in a planting bed in the rainproof shed of the Horticulture Center in National

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