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Rose E. Palumbo, Wai-Foong Hong, Jinguo Hu, Charles Krause, James Locke, Richard Craig, David Tay, and Guo-Liang Wang

Pelargonium is one of the priority genera collected by the Ornamental Plant Germplasm Center (OPGC). In order to protect future breeders from a loss of genetic diversity, the OPGC collects heirloom cultivars, breeding lines, and wild species. The current Pelargonium collection consists primarily of cultivars originating from P. ×hortorum and P. ×domesticum. Our project was designed to analyze the current collection in order to facilitate the maintenance of a more-diverse core collection. We have expanded our TRAP (Target Region Amplified Polymorphism) analysis from 120 plants with one primer set to include 780 plants with four primer sets. Each primer set consists of a labeled arbitrary primer paired with a gene-specific primer, and two different fluorescent labels were used to allow multiplexed PCR reactions. We scored about 90 markers in each of the first two primer sets and about 60 markers in each of the second two. In comparisons between the phylogeny and the morphology and taxonomy of these plants, we show some matching clusters that may be explained by the breeding history of the plants.

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Chengyuan Liu, Guoliang Wang, Hui Wang, Tao Xia, Shouzhou Zhang, Qigang Wang, and Yanming Fang

gene ( Qiu et al., 2012 ). Although Rosa species phylogeny has been researched using these different markers, when comparing with this notoriously complex genus, its resolution remains poor. Additionally, contradictory results exist between these

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Yingmei Gao, Jingkang Hu, Tingting Zhao, Xiangyang Xu, Jingbin Jiang, and Jingfu Li

, W. Nie, X. 2016 Genome-wide identification, phylogeny and expression analysis of AP2/ERF transcription factors family in Brachypodium distachyon BMC Genomics 17 1 636 Energy’s Joint Genome Institute 1997 Phytozome. 21 June 2017. < https

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Robert E. Marra

Taxonomy, Phylogeny, and Ecology of Bark-inhabiting and Tree-pathogenic Fungi in the Cryphonectriaceae. M. Gryzenhout, B.D. Wingfield, and M.J. Wingfield. 2009. APS Press, St. Paul, MN 55121. 136 pages, 14 color images, 38 black and white images

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B.S. Vivek and Philipp W. Simon

Current classifications of the genus Daucus are based on morphological and anatomical characteristics. We have used single to low copy nuclear restriction fragment length polymorphisms (nRFLPs) to describe the phylogeny and relationships of eight Daucus species including cultivated carrot (D. carota L.). Parsimony analysis of 247 characters (DNA fragments from 58 probe-enzyme combinations) yielded a tree in which accessions were grouped into three major clades and phenetic analysis using Jaccard's coefficient yielded two major clusters. The phylogenetic relationships from the nuclear RFLP data generally agreed with an earlier morphological classification. Resolution and placement of D. guttatus and D. muricatus were not consistent with the morphological classifications. Molecular variation among carrot inbreds was large.

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Keenan Amundsen and Scott Warnke

growing at a distance. The individual phylogenies were used to distinguish Agrostis accessions by species. Certain Agrostis species have been included in phylogenies of the Aveneae and Poeae tribes based on DNA sequence data, placing Agrostis species

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Joseph Tychonievich and Ryan M. Warner

, namely the elongated connective between the two thecae of the anthers ( Claßen-Bockhoff et al., 2003 ). The validity of this grouping was called into question by Walker et al. (2004) , who constructed a phylogeny based on rbcL and trnL-F gene

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Rose Palumbo, Wai-Foong Hong, Guo-Liang Wang, Jinguo Hu, Richard Craig, James Locke, Charles Krause, and David Tay

systematics and also sorted the phylogeny according to the gene of interest. Yang et al. (2005) reported using TRAP to detect markers for quantitative trait loci (QTL) mapping in wheat. They observed that TRAP produced more markers but not in the targeted

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Xiaomeng Li, Rangjin Xie, Zhenhua Lu, and Zhiqin Zhou

species. Citrus has a long history of cultivation—more than 4000 years ( Scora, 1988 ; Webber et al., 1967 ). However, the huge controversy over the phylogeny of key wild species, and the genetic relationship between the cultivated species and their

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Peter J. Zale and Pablo Jourdan

-resolved taxonomy or phylogeny does not yet exist. The taxonomic confusion is caused by extensive intraspecific variation prevalent in Phlox carolina and P. glaberrima , which has led some to refer to these as the P. carolina – P. glaberrima complex, but more