Photoinhibition (PI; light stress) was studied in leaves of 3-year-old, potted, mango plants, following changes in chlorophyll fluorescence characteristics. Iron deficiency was induced by calcareous soil mix. It was found that during summer days, the photochemical quantum yield (measured as Fv: Fm) was reduced by 15% to 30% in sun-exposed leaves by noon (under 2000 μmol·m–2·s–1). This apparent PI was mostly recovered by the next day. In Fe-deficient plants, the reversible PI was enhanced further. In addition, Fe-deficiency increased the “I” phase (i.e., the QB-nonreducing centers) and the non-photochemical quenching (qN) and reduced the photochemical quenching (qP). Photosynthesis rates in the deficient plants were half the control rates. Overnight chilling at 7C, which by itself did not affect the Fv: Fm ratio much, dramatically increased the susceptibility of the plants to photodamage on the following day (indicated by fluorescence and gas exchange assays). This postchilling PI was mostly irreversible but could slowly (within about 1 week) be recovered in a thermostated greenhouse. These results agree with our observations of field-grown Fe-deficient mango trees that develop severe necrosis and leaf-drop each autumn in the Jordan Valley when the days are still sunny and warm and the nights turn cold.
Gil Nir, Kira Ratner, Eugene E. Gussakovsky, and Yosepha Shahak
Marc W. van Iersel, Geoffrey Weaver, Michael T. Martin, Rhuanito S. Ferrarezi, Erico Mattos, and Mark Haidekker
-induced damage (photoinhibition), which decreases quantum yield of PSII. Higher plants have developed a complex set of responses to excess light, which allows them to safely dissipate excess light energy as heat (resulting in NPQ) and minimize photoinhibition
Xiang Wang, Rajeev Arora, Harry T. Horner, and Stephen L. Krebs
particularly vulnerable to photoinhibition or photo-oxidative damage ( Peng et al., 2008 ). Many plants have evolved mechanisms to dissipate excess absorbed light safely as thermal energy through the xanthophyll cycle ( Adams et al., 2004 ) and/or protect
Ryan N. Contreras, John M. Ruter, James S. Owen Jr., and Andy Hoegh
. Winter browning in japanese-cedar occurs through the conversion of chloroplasts to chromoplasts during winter ( Ida, 1981 ). This transition takes place only in sun-exposed leaves during periods of low temperature, indicating that photoinhibition likely
Mack Moyo, Manoj G. Kulkarni, Jeffrey F. Finnie, and Johannes Van Staden
-h photoperiod at a temperature of 25 °C were moved to continuous dark conditions at 25 °C resulting in the reversal of the photoinhibition effect ( Fig. 4 ). Similarly, 9-month-old seeds that did not germinate at 10, 15, and 20 °C showed
Jason J. Griffin, Thomas G. Ranney, and D. Mason Pharr
Tolerance to high solar irradiation is an important aspect of stress tolerance for landscape plants, particularly for species native to understory conditions. The objective of this study was to evaluate differential tolerance to high solar irradiation and underlying photosynthetic characteristics of diverse taxa of Illicium L. grown under full sun or 50% shade. Eleven commercially available taxa of Illicium were evaluated for light tolerance by measuring light-saturated photosynthetic capacity (Amax), dark-adapted quantum efficiency of photosystem II (Fv/Fm), and relative chlorophyll content using a SPAD chlorophyll meter. Comparisons of Amax indicated that three of the 11 taxa (I. anisatum L., I. parviflorum Michx. ex Vent., and I. parviflorum `Forest Green') maintained similar rates of light-saturated carbon assimilation when grown in either shade or full sun. All other taxa experienced a significant reduction in Amax when grown in full sun. Chlorophyll fluorescence analysis demonstrated that Fv/Fm was similar between sun and shade plants for the same three taxa that were able to maintain Amax. These taxa appeared to experience less photoinhibition than the others and maintained greater maximum photochemical efficiency of absorbed light. SPAD readings were not significantly reduced in these three taxa either, whereas most other taxa experienced a significant reduction. In fact, SPAD readings were significantly higher in I. parviflorum `Forest Green' when grown under full sun, which also maintained the highest Amax of all the taxa. These results suggest that there is considerable variation in light tolerance among these taxa, with I. parviflorum `Forest Green' demonstrating superior tolerance to high light among the plants compared. A more rigorous examination of I. parviflorum `Forest Green' (high light tolerance) and I. floridanum Ellis (low-light tolerance) demonstrated that I. parviflorum `Forest Green' had a considerably higher Amax, a higher light saturation point, greater potential photosynthetic capacity, reduced susceptibility to photoinhibition as indicated by superior PSII efficiency following light exposure, greater capacity for thermal de-excitation as indicated by a higher rate of nonphotochemical quenching (NPQ) under full sun, greater apparent electron transport rate (ETR) at mid-day, and higher concentrations of the free-radical scavenger myo-inositol. All of these factors contribute potentially to a greater capacity to use light energy for carbon fixation while minimizing photodamage.
Anthony W. Whiley, Christopher Searle, Bruce Schaffer, and B. Nigel Wolstenholme
Leaf gas exchange of avocado (Persea americana Mill.) and mango (Mangifera indica L.) trees in containers and in an orchard (field-grown trees) was measured over a range of photosynthetic photon fluxes (PPF) and ambient CO2 concentrations (Ca). Net CO2 assimilation (A) and intercellular partial pressure of CO2 (Ci) were determined for all trees in early autumn (noncold-stressed leaves) when minimum daily temperatures were ≥14 °C, and for field-grown trees in winter (cold-stressed leaves) when minimum daily temperatures were ≤10 °C. Cold-stressed trees of both species had lower maximum CO2 assimilation rates (Amax), light saturation points (QA), CO2 saturation points (CaSAT) and quantum yields than leaves of noncold-stressed, field-grown trees. The ratio of variable to maximum fluorescence (Fv/Fm) was ≈50% lower for leaves of cold-stressed, field-grown trees than for leaves of nonstressed, field-grown trees, indicating chill-induced photoinhibition of leaves had occurred in winter. The data indicate that chill-induced photoinhibition of A and/or sink limitations caused by root restriction in container-grown trees can limit carbon assimilation in avocado and mango trees.
Ryan N. Contreras, Ron Determann, and Mara Friddle
screens or specimens in landscapes. However, winter browning caused by photoinhibition ( Ida, 1981 ) is unsightly and likely has reduced the use of japanese-cedars. Winter browning occurs as a result of the accumulation of the pigment rhodoxanthin during
Richard T. Olsen, John M. Ruter, and Mark W. Rieger
Illiciums, or star-anises, have increased in popularity in the nursery and landscape industries. However, confusion exists as to which taxa are tolerant of high light intensities during production and subsequent establishment in the landscape. We investigated the effect of two light intensity treatments, 45% and 100% full sunlight, on gas-exchange parameters of five Illicium taxa: Illicium anisatum L., I. floridanum Ellis. `Pebblebrook', I. henryi Diels., I. lanceolatum A.C. Sm., and I. parviflorum Michx. Ex. Vent. `Forest Green'. Light-response curves were determined for individual leaves, and mean response parameters calculated. Chlorophyll and total carotenoids were analyzed after extraction in acetone, with total chlorophyll also estimated with a SPAD chlorophyll meter. In general, highest rates of CO2 assimilation (Amax) and lowest rates of dark respiration (Rd) were found in the 45% light treatment for all taxa. Both Illicium anisatum and I. floridanum `Pebblebrook' had substantial reductions in Amax in 100% light, 94% and 81% respectively, compared to plants grown in the 45% light treatment. Illicium henryi failed to survive the 100% light treatment. Illicium lanceolatum and I. parviflorum `Forest Green' were least affected by the 100% light treatment. Severe photooxidative bleaching was noted and confirmed by SPAD and pigment data, although SPAD readings were a poor predictor of total chlorophyll. For taxa of Illicium in our study, photosynthetic gas-exchange parameters and foliage pigment characteristics were improved in the low light treatment, suggesting optimal growth occurs in shaded conditions.
Rufaro Madakadze, Ellen M. Chirco, and Anwar A. Khan
The effect of preplant conditioning on germination of three flower seeds, Bupleurum griffithii (Tourn.) L. (thorough-wax), Ammi majus L. (greater ammi), and Cirsium japonicum DC. Per. (Japanese thistle), were studied. Seeds were osmoconditioned with -1.2 MPa polyethylene glycol 8000 (PEG) solution and matriconditioned with moist Micro-Cel E (ratio of 2 seed: 0.6 carrier: 3 water by weight for Bupleurum and Cirsium; for Ammi the ratio was 2:1.4:6) and moist expanded vermiculite #5 (the ratio was of 2 seed: 0.6 carrier: 2 water for Bupleurum). In some treatments, water in the matriconditioning mixture was replaced with 1 mm gibberellin A4+7 (GA) or 0.2 % KNO3. In Bupleurum, matriconditioning with Micro-Cel E was generally superior to matriconditioning with vermiculite or osmoconditioning with PEG. A 4-day matriconditioning with Micro-Cel E and germination in the dark reduced the period required for 50% (T50 of final germination by 4 days and improved the percentage germination at 20C (73 % vs. 95%), compared to nonconditioned seeds germinated in the dark. The treatment also improved the percentage of germination at 15C (68% vs. 95%) and effectively removed the thermoinhibition of germination at 25 and 30C. Germination was inhibited to a greater extent for seeds kept in the light during matriconditioning and germination than for seeds conditioned in darkness and germinated in light or conditioned in light and germinated in darkness. Nitrate added during conditioning in light prevented inhibition of germination, provided seeds were kept in darkness during germination. In A. majus, germination in light after 4-day matriconditioning reduced the T50 by ≈2 days, but had little effect on percentage germination. Both GA and irradiance equally promoted germination when added during osmoconditioning, with nitrate having no effect. In C. japonicum, a 4-day matriconditioning or a 7-day osmoconditioning reduced the T50 of germination by -2 days and improved the percentage germination to some extent. Neither irradiance nor nitrate had any significant effect.