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Christine M. Rainbolt, Jayesh B. Samtani, Steven A. Fennimore, Celeste A. Gilbert, Krishna V. Subbarao, James S. Gerik, Anil Shrestha, and Bradley D. Hanson

intensity, diversity, and high capital costs inherent in this cropping system have led to a reliance on MB fumigation for preplant control of a broad range of soilborne pathogens, weeds, and nematodes. MB was classified as a Class I stratospheric ozone

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Diane Feliciano Cayanan, Ping Zhang, Weizhong Liu, Mike Dixon, and Youbin Zheng

irrigation water and leachate into a reservoir until the water is needed again for irrigation. However, the risk of spreading plant pathogens found in recycled irrigation water is a deterrent for many operations ( Bush, 2002 ; Bush et al., 2003 ; Ehret et

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Naveen Hyder, James J. Sims, and Stephen N. Wegulo

resource ( Masago et al., 1977 ; Meerow, 1994 ; Prasad, 1997 ). It has been demonstrated that coir can suppress certain soilborne plant pathogens ( Candole and Evans, 2004 ; van der Gaag and Wever, 2005 ). Combining the disease-suppressive properties of

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Adam Dale, Becky R. Hughes, and Danielle Donnelly

Micropropagation has been used successfully for strawberry ( Fragaria × ananassa L.) for over 30 years ( Boxus, 1974 ; Swartz and Lindstrom, 1986 ). This has been applied, often together with thermotherapy, to produce specific pathogen

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Ron R. Walcott

Plant pathogens present a serious threat to seedling establishment and the potential for plant disease epidemics under greenhouse conditions is great. Hence, pathogen exclusion by detection and elimination of infested seedlots remains a requisite tactic for seedling production and disease management. Unfortunately, the numbers of contaminated seed within a lot may be low and infested seed may be asymptomatic making their detection difficult. To address these issues seed detection assays have been developed, but many of them have shortcomings that reduce their effectiveness. Examples of frequently used seed assays include visual examination, selective media, seedling grow-out and serological assays which, while appropriate for some pathogens, often display inadequate levels of sensitivity and specificity. Recently, the polymerase chain reaction (PCR) has emerged as a tool for detecting microorganisms in many diverse environments. Thus far, it is clear that DNA-based detection systems exhibit higher levels sensitivity than conventional techniques. Unfortunately, PCR-based seed tests require the extraction of PCR-quality DNA from target organisms in backgrounds of saprophytic organisms and inhibitory seed-derived compounds. The inability to efficiently extract PCR-quality DNA from seeds has restricted the acceptance and application of PCR for seed detection. To overcome these limitations several modified PCR protocols have been developed including selective target colony enrichment followed by PCR (BIO-PCR) and immunomagnetic separation and PCR. These techniques seek to selectively concentrate or increase target organism populations to enhance detection and have been successfully applied for detecting bacteria in seed. Other techniques with great potential for rapid detection of seedborne pathogens include magnetic capture hybridization and PCR, and DNA-chip technology. Ultimately, PCR will be available for the detection of all seedborne pathogens and may supersede conventional detection methods.

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Brianna L. Ewing and Barbara A. Rasco

when fruit is shaken and allowed to drop from the tree, fruit must be processed immediately, as it will rot more rapidly ( Alexander et al., 2016 ; Miles and King, 2014 ). Furthermore, fruit contact with the orchard floor may raise pathogen

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Jacqueline Joshua and Margaret T. Mmbaga

Fungal pathogens impose major constraints on agricultural production globally ( Collinge et al., 2010 ). Disease management strategies have relied heavily on conventional chemical fungicides. Persistent challenges associated with the use of

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Joseph L. Smilanick

Postharvest Pathogens and Disease Management . 2005. P. Narayanasamy. John Wiley, Somerset, NJ. 578 pages. $135.00. Hardcover. ISBN: 978-0-471-74303-3 Postharvest diseases are responsible for the spoilage of durable and fresh perishable

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Xiuling Tian and Youbin Zheng

-borne plant pathogens within the recirculation system ( Richard et al., 2006 ). Various water disinfection technologies have been used in controlled environment plant production systems including greenhouse and nursery operations. However, these technologies

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Anas Eranthodi, Mohammad Babadoost, and Bernhard Trierweiler

to plant in the next season. Most of the sets that the producers save are apparently healthy (asymptomatic), but they are often infected with Verticillium and Fusarium spp. ( Babadoost, 2006 ). Set transmission of these pathogens is important