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Beibei Li, Jianfu Jiang, Xiucai Fan, Ying Zhang, Haisheng Sun, Guohai Zhang, and Chonghuai Liu

) Standl.] landraces revealed by simple sequence repeat markers HortScience 51 120 126 Mihaljevic, M.Z. Simon, S. Pejic, I. Carka, F. Sevo, R. Kojic, A. Preiner, D. 2013 Molecular characterization of old local grapevine varieties from south east European

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Fokar Mohamed and Richard Durham

Agrobacterium vitis is the causal organism of crown gall in grapevine. Infection is particularly severe in areas that experience winter damage to vines. Improving resistance to A. vitis will require a detailed knowledge about this organism. In this study, 18 grapevine isolates of A. vitis were collected from different locations near Lubbock, Texas. Isolates were subjected to a phenotypic characterization using 12 biochemical tests, including production of alkali from L-tartrate, production of 3-ketolactose, utilization of citrate, and others. Previously characterized isolates of A. vitis and A. tumefaciens obtained from the American Type Culture Collection served as positive and negative controls in these assays. Isolates were also evaluated for host range, tumor morphology, and opine utilization, and were compared at the molecular level by restriction fragment length polymorphism analysis of the oncogenic regions of the T-DNA plasmid. Although all isolates were able to metabolize tartrate and grow on Roy–Sasser media, there was much variability based on other tests. Twelve of 18 isolates were able to utilize octopine as a sole carbon source. All isolates tested thus far have been pathogenic on tomato and tests on grapevines are underway.

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M.J. Prado, S. Romo, M. Novo, M. Rey, M.T. Herrera, and M.V. González

We investigated the characterization of genotypes of Actinidia deliciosa (Chev.) Liang and Ferguson var. deliciosa by using isozymatic and molecular techniques [randomly amplified polymorphic DNA (RAPD), amplified fragment-length polymorphism (AFLP), standard AFLP, and modified AFLP]. Four genotypes were tested, the female cultivar `Hayward', the traditional New Zealand pollinizers `Matua' and `Tomuri', and a new pollinizer named clone A selected in a breeding program in Spain. PGI and PGM were the only isozymes that allowed us to distinguish the kiwifruit genotypes, although the accessions of `Matua' presented two different banding patterns for both isozymes. All three molecular markers differentiated between the genotypes of kiwifruit tested, although RAPD markers did not allow us to establish differences between accessions of `Matua', while both standard and modified AFLP did. These results, along with those of isozymes, support the hypothesis that the male kiwifruit genotypes present in Europe belong to different clones. None of the markers used showed differences between accessions of `Hayward', which would suggest that it is a uniform cultivar. On the other hand, clone A was a seedling derived from `Hayward' and an unknown pollinizer. The results obtained using AFLP markers strongly suggest that `Tomuri' may have been the male parent of clone A. A specific protocol for kiwifruit characterization based on a modified AFLP technique is also presented, that gave rise to the highest percentage of polymorphism while scoring the lowest number of bands. This, together with the technical features of modified AFLP markers, make them very useful for identifying propagated kiwifruit plant material in commercial nurseries.

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Salih Kafkas, Yıldız Doğan, Ali Sabır, Ali Turan, and Hasbi Seker

polymorphism detected by the three marker systems, the number of primers used, and the plant species may also affect the correlations among the different markers. Several types of molecular markers are available for plant germplasm characterization. Comparisons

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Maria Jose Gonzalo, Elisabet Claveria, Antonio J. Monforte, and Ramon Dolcet-Sanjuan

-induced parthenogenesis by pollination with γ-irradiated pollen followed by in vitro embryo rescue; second, to generate a DHL population from a selected hybrid; and third, to perform its molecular characterization. Materials and Methods Plant material and pollination with

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Àngel Fernández i Martí, José M. Alonso, María T. Espiau, María J. Rubio-Cabetas, and Rafel Socias i Company

, molecular identification using DNA markers has become the main tool for the characterization and management of the germplasm collections of most fruit species. For the Prunus genus, such studies were first carried out using isozymes, such as in peach

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Rosanna Freyre and Erin Tripp

artificial crosses between Ruellia simplex and R. caroliniensis to test whether the two taxa could be artificially hybridized. Hybrids obtained were analyzed both morphologically and molecularly. Results from this investigation should be of interest to

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Rayane Barcelos Bisi, Rafael Pio, Daniela da Hora Farias, Guilherme Locatelli, Caio Morais de Alcântara Barbosa, and Welison Andrade Pereira

incompatibility between cultivars. In addition to the physiological aspects evaluated, this strategy, which is associated with molecular characterization data of S-alleles, facilitates the understanding of the reproductive system and the choice of more compatible

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Rohollah Karimi, Ahmad Ershadi, Kourosh Vahdati, and Keith Woeste

small number of individuals Genetics 89 583 590 Nicese, F.P. Hormaza, J.I. McGranahan, G.H. 1998 Molecular characterization and genetic relatedness among walnut ( Juglans regia L.) genotypes based on RAPD markers Euphytica 101 199 206 Potter, D. Gao, F

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Zhong-Bin Wu, Hsin-Mei Ku, Yuh-Kun Chen, Chung-Jan Chang, and Fuh-Jyh Jan

etiology of this chlorotic spot disease of pear has not been clarified. The objective of this study was to identify and characterize the causal agent of this pear disease. We report here the isolation, serological and molecular characterizations of the