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and graft genotypes have been successfully micropropagated. The micropropagation process is subject to multiple influencing factors, including genetic background of the tree, the type of tissue being used as an explant, the concentration of growth

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in vitro micropropagation. Micropropagation of wild plants, however, can be labor-intensive and time-consuming owing to the difficulties of removing microbial contamination and of avoiding extensive oxidation of the explants. To overcome such problems

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period of time. In vitro propagation of S. rebaudiana has been studied by many researchers to develop an efficient and economic micropropagation protocol in semisolid medium ( Ahmed et al., 2007 ; Giridhar et al., 2010 ; Hwang, 2006 ; Lata et al

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this study was to develop an efficient method for micropropagation of C. formosanum using explants from adult plants. The effects of timing for explant collection, BA and thidiazuron (TDZ) concentrations on shoot multiplication, and organic

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area and shoot and root dry weight were, respectively, 5.2, 4.6, and 3.8 times higher than those cultured under a conventional micropropagation system. Moreover, plantlets produced photoautotrophically have been shown to acclimatize better in ex vitro

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study were to develop a reliable micropropagation protocol for threatened Georgia aster using seeds to start cultures and to investigate the feasibility of long-term seed storage through cryopreservation. Materials and Methods Plant materials

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for sale, very little literature is available concerning the propagation of Sarracenia plants using micropropagation techniques. Several researchers have attempted to develop in vitro propagation methods for Sarracenia species ( Arnold, 1989

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breeding ( Portillo et al., 2007 ). Therefore, the most effective means for genetic improvement is through biotechnology. Micropropagation systems and regeneration systems through either organogenesis or somatic embryogenesis have already been established

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Abstract

In vitro shoot-tip culture of grapevine (Vitis vinifera L.) was used first to eliminate some viruses (6) and, more recently, for rapid micropropagation (2-4). In vitro culture was successful when the shoot tips were taken from plants grown in glasshouse or shaded conditions. Shoot tips from field grown plants died within 2 days of culture (5).

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159 ORAL SESSION 45 (Abstr. 687–693) Micropropagation–Floriculture/Ornamental Horticulture

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