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Genet Teshome Mekuria, Margaret Sedgley, Graham Collins, and Shimon Lavee

A sequence-tagged site (STS) was developed to identify a genetic marker linked to resistance to olive leaf spot caused by the pathogen, Spilocea oleaginea (Cast) (syn. Cycloconium oleaginum Cast.). The STS was based on a randomly amplified polymorphic DNA (RAPD) marker of about 780 base pairs (bp) linked to olive leaf spot resistance. Several primer pairs were developed to flank the sequence, and one pair produced the expected polymorphism between resistant and susceptible individuals tested, and was used as an STS marker. This primer pair was tested against parents and 34 individuals from a population segregating for resistance to olive leaf spot, and 12 commercial olive (Olea europaea L.) cultivars showing various levels of resistance to the disease. The STS marker was present in 71.4% of the parents and progeny that were designated as resistant, and was absent in 87% of the parents and progeny showing susceptibility. These primers were also able to distinguish cultivars such as `Koroneiki' and `Leccino', that are reported to show resistance to olive leaf spot, from `Barouni' and `Mission', that are reported to be susceptible. This is the first report of a STS marker for olive, and its use will assist greatly in screening olive progeny for resistance to leaf spot in breeding programs.

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Meryem Ipek, Ahmet Ipek, Douglas Senalik, and Philipp W. Simon

such as rate of crop maturity, bulb size and color, clove size and number, leaf number and color, and bolting habit. Variation observed in genetic diversity studies using molecular markers is as wide as that observed for phenotypic variation among the

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Mary Woodhead, Ailsa Weir, Kay Smith, Susan McCallum, Katrin MacKenzie, and Julie Graham

generated in a crossing program. In red raspberry, it can take 15 years from the time of the first cross to the release of a new cultivar ( Graham and Jennings, 2009 ). Advances in molecular genetics, in particular, the development of molecular markers, have

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Paul Skroth, Jim Nienhuis, Geunhwa Jung, and Dermont Coyne

30 POSTER SESSION 4 (Abstr. 460-484) Breeding/Genetics/Molecular Markers

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V. Meglic and R.T. Chetelat

57 WORKSHOP 8 Use of Molecular Markers in Germplasm Management

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Kahraman Gürcan and Shawn A. Mehlenbacher

variation in chromosome number and genome size among species of the Betulaceae, some degree of microsatellite marker transferability is expected based on results in other plant families. Microsatellites, also known as simple sequence repeats (SSR), are

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Kimberly J Walters, George L. Hosfield, and James D. Kelly

30 POSTER SESSION 4 (Abstr. 460-484) Breeding/Genetics/Molecular Markers

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Mohammad Sadat-Hosseini, Kourosh Vahdati, and Charles A. Leslie

cytometry, molecular markers, and biochemical and phenotypic analyses ( Bradaï et al., 2016 ; Harding, 2004 ; Sadat-Hosseini et al., 2011 ). Molecular analyses and flow cytometry have been used in many species to evaluate the trueness-to-type of plantlets

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Shanthanu Krishna Kumar, Nathan Wojtyna, Laura Dougherty, Kenong Xu, and Gregory Peck

) marker, named Q8 , was developed, which is physically located at 11.1 Mb between genes MdPP2CH (8.7 Mb) and MdSAUR37 (11.6 Mb) on chromosome 8 in the GDDH13 apple reference genome ( Daccord et al., 2017 ; Jia et al., 2018 ). Additionally, three more

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Thomas Horejsi and Jack Staub

106 POSTER SESSION (Abstr. 335–343) Breeding and Genetics–Vegetables II (Molecular Markers and Physiological Genetics)