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includes maximum 5% or 5% to 10% area with defects, respectively, and noncommercial fruit have no sound peduncle area or >10% area with defects. Fig. 1. ( Top ) Fruit from near-isogenic lines (NILs) of Cucumis melo at harvest showing different

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appropriate populations, such as recombinant inbred lines, advanced backcross populations ( Tanksley and Nelson, 1996 ), and genomic libraries of near-isogenic lines (NILs; Eshed and Zamir, 1995 ), which allow for an accurate cartography of quantitative

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of QTLs using different population structures ( Tanksley, 1993 ) as well as the development of populations suitable for QTL analysis, such as introgression lines and near-isogenic lines (NILs). A NIL genomic library consists of a set of lines, each

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levels. Materials and Methods Plant material, backgrounds, and introgressions. The near isogenic lines (NILs) of tomato differing in the introgression source of the distal portion of chromosome 1 were developed from the interspecific cross

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an elite genetic background ( Tanksley and McCouch, 1997 ). Approaches to identify the quality trait loci (QTLs) responsible for low-temperature responses in the introgressed region of near-isogenic lines (NILs) of tomato ( Solanum lycopersicon L

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Abstract

The effects of N rate on tipburn occurrence in cabbage (Brassica oleracea L. Capitata group) and on concentrations of inorganic elements in cabbage plants were determined in semi-isogenic inbred lines of cabbage that were resistant and susceptible to tipburn. Resistant inbred lines had very little tipburn in the heads at harvest time, but nearly all of the heads of susceptible lines had tipburn on internal leaves. Addition of N slightly increased tipburn. Early in the growing season, leaves of plants of the resistant inbred lines were higher in concentrations of total N, NO3-N, K, Cl, and Fe, but lower in Zn and Cu than for susceptible lines. At harvest, heads of the resistant inbred lines were higher in concentrations of Ca, SO4-S, and NO3-N, but lower in P, K, Na, and Zn than for heads of susceptible lines.

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The potato (Solanum luberosum, L.) is the fourth most important food crop worldwide and the most important vegetable crop in the United States. The United States is the only country which prefers russet skin potatoes, but plant breeders experience difficulty in manipulating the trait, since the potato is an autosomal tetraploid with complex inheritance. The enzyme banding patterns of white and russet skin potato tubers from five near-isogenic lines were studied in order to identify specific isozyme differences associated with russeting. Tuber skin samples were prepared and run on polyacrylamide gels. The gels were then stained for eight enzyme systems (PRX, EST, MDH, PGM, PGI, GOT, G-6PDH, 6PGDI-I) and scored for banding differences. Peroxidase activity was always associated with russeting, and differences in esterase banding were also observed. Potential markers associated with russeting might have been found, which could provide fingerprinting tools for mapping the gene(s) responsible for russeting in potato.

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Abstract

Differences in water absorption by intact seeds and in osmotic properties of excised seed coats were measured in 4 near-isogenic breeding lines of snap bean, Phaseolus vulgaris L. White seeds absorbed water more rapidly than colored seeds. Excised white seed coats were more permeable to water than colored seed coats in response to an osmotic gradient. Seed coat thickness and seed coat dry weight were negatively correlated with rate of osmosis through the seed coats. Colored seeds had greater seed coat dry weight and thickness than white-seeded isolines.

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Two pairs of near-isogenic lines of green-sprouting broccoli ( Brassica oleracea L. var. italica ), designated USVL115-GL and USVL115-NG, and USVL188-GL and USVL188-NG, were released by the Agricultural Research Service of the U.S. Department of

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Two isogenic sets of bell pepper (Capsicum annuum L.) lines (differing at the N root-knot nematode resistance locus) were characterized for resistance to Meloidogyne arenaria (Neal) Chitwood races 1 and 2, M. hapla Chitwood, and M. javanica (Treub) Chitwood in greenhouse and growth chamber tests. The isogenic sets of C. annuum were `Charleston Belle' (NN) and `Keystone Resistant Giant' (nn-recurrent parent), and `Carolina Wonder' (NN) and `Yolo Wonder B' (nn-recurrent parent). Meloidogyne arenaria race 1 is pathogenic to C. annuum. `Charleston Belle' and `Carolina Wonder' exhibited high resistance to M. arenaria race 1. Their respective recurrent backcross parents, `Keystone Resistant Giant' and `Yolo Wonder B', were susceptible to M. arenaria. Meloidogyne arenaria race 2 and M. javanica are not highly pathogenic to pepper. However, `Charleston Belle' and `Carolina Wonder' both exhibited higher (P≤0.05) resistance to M. arenaria race 2 and M. javanica than `Keystone Resistant Giant' and `Yolo Wonder B'. Meloidogyne hapla is pathogenic to pepper. Both `Charleston Belle' and `Carolina Wonder' and their respective recurrent parents, `Keystone Resistant Giant' and `Yolo Wonder B', were susceptible to M. hapla. We concluded that the N gene confers resistance to M. arenaria races 1 and 2, and M. javanica in C. annuum, but the N gene does not condition resistance to M. hapla.

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