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Cecil Pounders, Tim Rinehart and Hamidou Sakhanokho

interspecific hybrid cultivars ( Egolf, 1981a , 1981b , 1986a , 1986b , 1987 , 1990 ; Pooler, 2006a ; Pooler and Dix, 1999 ) that successfully combined the powdery mildew resistance of L. fauriei with other desirable horticultural traits from L. indica

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Ming Cai, Ke Wang, Le Luo, Hui-tang Pan, Qi-xiang Zhang and Yu-yong Yang

et al. (2001) . Other interspecific crosses between H . macrophylla and H . arborescens or H . macrophylla and H. quercifolia failed because of postzygotic barriers. The putative hybrid seedlings died at the cotyledonary stage or the first

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Desire Djidonou, Amarat H. Simonne, Karen E. Koch, Jeffrey K. Brecht and Xin Zhao

-resistant and interspecific tomato hybrid rootstocks have also been developed ( King et al., 2010 ). Moreover, vegetable grafting has been used successfully to minimize the deleterious effects of a wide range of abiotic stresses related to salinity ( Colla et al

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Chalita Sriladda, Heidi A. Kratsch, Steven R. Larson, Thomas A. Monaco, FenAnn Shen and Roger K. Kjelgren

: aesthetic and extreme drought tolerance qualities of S. rotundifolia but greater tolerance of wet, disturbed soils and faster growth of S. argentea . This study describes an interspecific hybrid between the two species in terms of genetics and leaf

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Michael Kantar, Kevin Betts, Brent S. Hulke, Robert M. Stupar and Donald Wyse

; Sacks et al., 2003 ; Wang et al., 2009 ). One of the promising wild species being investigated for creating a perennial sunflower crop is H. tuberosus . Interspecific hybrids between H. annuus and H. tuberosus also show promise as breeding material

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Young-ju Kim and David H. Byrne

The hybrid origin of 23 rose (Rosa spp.) accessions was examined with three isozymes: acid phosphatase (E.C.3.1.3.2), malate dehydrogenase (E.C.1.1.1.37), and phosphoglucose isomerase (E.C.5.3.1.9). All three isozymes were useful for interspecific hybrid verification. This procedure was effective if the putative parents were known and differed in isozyme phenotype. To verify the origin of hybrid species or cultivars with hybrid origins, isozymes were useful but limited by the number of generations since the original hybridization and the number of accessions of the putative parental species assayed.

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Daniel H. Ronis, Anson E. Thompson, David A. Dierig and Earl R. Johnson

Five interspecific Cuphea hybrids were examined for isozyme banding patterns. In three of the five hybrids (C. viscosissima × C. lutea, C. ignea × C. angustifolia, and C. lanceolata × C. viscosissima), F1 plants could be distinguished from either parental species. Phosphoglucomutase and 6-phosphogluconic dehydrogenase enzyme stains produced distinct F1 band patterns for all three hybrids. Phosphoglucose isomerase and shikimate dehydrogenase enzyme stains produced distinct F1 band patterns for C. viscosissima × C. lutea and C. lanceolata× C. viscosissima, respectively. For the C. lanceolata × C. viscosissima hybrid. the banding patterns were used to identify 32 selfs among 161 putative F1 plants.

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Thaddeus McCamant and R. Alan Black

Cold hardiness was studied in two interspecific Populus hybrids (P. trichocarpa × P. deltoides, and P. trichocarpa × P. maxomowiscii), using laboratory freezing tests of mid-winter dormant tissues and fully expanded leaves in the autumn. These laboratory measurements were compared to field observations. Hybrids having one parent from southern-source populations and the other parent from northern sources were compared to hybrids in which both parents were from southern-source populations. Populus hybrids with one parent of northern origin were generally hardier than hybrids from parents of southern sources; however, significant differences in cold hardiness were detected between hybrids having the same genetic parents. Field observations generally supported laboratory measurements and showed clonal differences in mid-winter cold hardiness and autumn leaf frost tolerance. Fully expanded leaves of different clones from the same parent also exhibited differences in frost tolerance.

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Madeline Wimmer, Beth Ann Workmaster and Amaya Atucha

2016 harvests for ‘Brianna’, ‘Frontenac’, ‘La Crescent’, and ‘Marquette’ cold climate, interspecific hybrid grape cultivars trained in high cordon (HC), Scott Henry (SH), and vertical shoot positioning (VSP) systems. z Fruit chemistry. There were very

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Yan Ma, David H. Byrne and Jing Chen

An objective of our rose breeding research is to transfer resistance to blackspot and other diseases from wild diploid species to modern rose cultivars. Interspecific hybrids among blackspot-resistant diploid species were chosen for chromosome doubling to produce fertile amphidiploids that could be hybridized to the tetraploid commercial germplasm. Five such F1 interspecific hybrids were treated with colchicine. The study included two different application procedures (shake in colchicine solution or colchicine in media), four colchicine concentrations (0.05%, 0.1%, 0.15%, and 0.20%), and five treatment periods (1, 3, 5, 8, and 10 days). After colchicine treatment, all the materials were cultured in vitro. One thousand-thirty-seven surviving explants were selected for typical “gigas” characteristics of doubled diploids. Chromosome counts on shoot tips of these selected genotypes confirmed 15 amphidiploids. The best colchicine treatment varied among the interspecific hybrids. Higher colchicine concentrations or duration reduced growth rating, rooting, and percent survival. The recognition of amphiploids and ploidy chimeras from young seedlings will also be discussed.