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Guochen K. Png, Katherine S. Downes, and Beng H. Tan

cuttings to optimize propagation success. Shoot micropropagation, the most common in vitro propagation system, provides an alternative method of propagating plants that is time-consuming or difficult to propagate otherwise ( Offord et al., 2009 ). The

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Dinum Perera and Brian W. Trader

development of in vitro protocols for plant regeneration through either organogenesis or somatic embryogenesis is one of the main prerequisites for the potential applications of clonal propagation and genetic transformation ( Vila et al., 2003 ). In vitro

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Carlos Alberto Lecona-Guzmán, Sheila Reyes-Zambrano, Felipe Alonso Barredo-Pool, Miguel Abud-Archila, Joaquín Adolfo Montes-Molina, Reiner Rincón-Rosales, and Federico Antonio Gutierrez-Miceli

protocol could be applied for massive plant propagation purposes for alcohol, fiber, and secondary metabolite production. Callus culture of A. americana could be a prerequisite for many techniques for in vitro manipulation, cellular line selection, and

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Bärbel Röck-Okuyucu, Meltem Bayraktar, Ismail Hakki Akgun, and Aynur Gurel

high labor costs for large-scale production ( Brandle et al., 1998 ; Gantait et al., 2015 ). A genetically homogeneous population, which produces high yields of the desired SGs, can be achieved by in vitro propagation of a selected plant ( Gantait et

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Xiuli Shen, Guochen Yang, and Zhongge (Cindy) Lu

). However, known for its notoriously slow growth and challenge to propagate, Alexandrian laurel is not readily available and rarely seen commercialized ( Matt, 2005 ). As an initiative part of a research program toward efficient in vitro propagation and

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Ana V. de Souza, José E.B.P. Pinto, Suzan K.V. Bertolucci, Ricardo M. Corrêa, Larissa C. do B. Costa, and William E. Dyer

vitro propagation of medicinal plants is clearly on the rise, because they represent the most important sources of medicines and other pharmaceutical products. Methods of in vitro propagation offer highly effective tools for germplasm conservation and

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Maria Papafotiou and Aekaterini N. Martini

. polium has low seed germination ( Nadjafi et al., 2006 ). The latter, being an endangered species in many countries of the Middle East, where it is widely used in traditional medicine, has been studied as for its possibility for in vitro propagation from

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Rongpei Yu, Ying Cheng, Yanfei Pu, Fan Li, and Shugang Lu

). Unfortunately, because of its overexploitation, populations of S. pulvinata have decreased sharply. Therefore, there is an urgent need to establish the in vitro propagation of S. pulvinata to produce high-quality plantlets for horticultural and medicinal use

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Wagner A. Vendrame, Ian Maguire, and Virginia S. Carvalho

have been published ( Arditti and Ernst, 1993 ). Particularly for Doritaenopsis , in vitro propagation protocols have been developed using different explants and various concentrations of plant growth regulators. Explants used include flower stalk

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Keun H. Cho, Veronica Y. Laux, Nathan Wallace-Springer, David G. Clark, Kevin M. Folta, and Thomas A. Colquhoun

adventitious rooting, shoot growth, and leaf color transition of coleus plants grown from vegetative stem cuttings, as well as establishment of in vitro shoot tip cultures. These experimental results demonstrate that propagation of coleus may be hastened by