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Daniel Potter, Fangyou Gao, Giovanna Aiello, Charles Leslie and Gale McGranahan

The utility of intersimple sequence repeat (ISSR) markers for identification of English or Persian walnut (Juglans regia L.) cultivars was explored. Four cultivars were screened with 47 ISSR primers; eight of these primers, which generated reproducible and informative data, were selected for further study. Two individuals from each of 48 cultivars, including many currently important in the California walnut industry as well as accessions from Europe and Asia, were then examined with the eight ISSR primers. Polymerase chain reaction (PCR) products were separated on agarose gels and stained with ethidium bromide. Fifty-four bands were scored as present or absent in each cultivar; of these, 31 (57%) were polymorphic among the 48 cultivars. Combined data from the eight ISSR primers provided a unique fingerprint for each of the cultivars tested. Fifteen of the cultivars could be distinguished from all others with just one primer, 31 with a minimum of two primers, and two required three primers. Pairwise genetic distances between the cultivars were calculated and a dendrogram was generated using the neighbor-joining algorithm. Some of the groupings in the dendrogram corresponded to groups which, based on known pedigrees, are genealogically closely related. Others included accessions from diverse genetic and/or geographic origins. These results can be attributed to a combination of the limitations of the ISSR method for inferring genetic relationships, on the one hand, and the complex history of walnut cultivar development involving extensive exchange and breeding of germplasm from different geographic regions, on the other.

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Mario I. Buteler, Don LaBonte and Raul Macchiavelli

The breeding of new sweetpotato varieties is a highly inefficient process, confounded by incompatibility, poor fertility, open-pollination, and its hexaploid nature. Upwards of 12 to 20 lines are currently combined in open-pollinated nurseries based on good horticultural characteristics. Most progeny after several years of selection can be traced back to just three or four maternal lines. A method that would identify the paternal parent of superior progeny would enable breeders the ability to combine parents that exhibit superior combining ability in more-efficient, smaller nurseries. The objective of this work is to explore by means of computer simulation the application of genealogy reconstruction techniques on hexaploid individuals with PCR-based data. The progeny obtained on each female parent is fractionally assigned to each male with non zero exclusion probability proportional to its paternity likelihood. Computer simulations show that at least five different alleles per loci are needed to reach a reasonable discriminatory level. Also, the number of loci scored should not be less than 20. An increment in the number of alleles or loci increases the discriminatory power; but, the number of alleles produces a far more important effect than the number of loci.

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Eun Ju Cheong, Myong-Suk Cho, Seung-Chul Kim and Chan-Soo Kim

. × yedoensis (“Wang-beot-na-mu” in Korean, but without known genealogical records) were extensively planted during the Japanese occupation, and they remain one of the most favorite trees planted along roadsides and in residential landscapes. In spite of its

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Joan Casals, Lluís Bosch, Francesc Casañas, Jaime Cebolla and Fernando Nuez

desired traits, so we designed a breeding program based on the genealogical method applied to the variability generated by crossing materials that had complementary traits ( Allard, 1999 ). We selected six lines with good scores for the ideotype

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Qi Zhang, Andy Medina and Chuck Lyerly

., Westport, CT. Zhang, Q. 2002 Genealogy and Breeding Method(s) of One Ball F. Plant Variety Protection Office of the United States of America. No. 200200107 Zhang, Q. 2012 Development of interspecific-bridge lines among Cucurbita pepo, C. maxima , and C

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Qi Zhang, Andy Medina and Christopher Lyerly

Publishing Company, Inc., Westport, CT Zhang, Q. 2012 Genealogy and breeding method(s) of Jill-Be-Little. Plant Variety Protection Office of the United States of America. No: 201200095 Zhang, Q. Yu, E. Medina, A. 2012 Development of interspecific-bridge lines

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Haofeng Chen, Vanessa E.T.M. Ashworth, Shizhong Xu and Michael T. Clegg

and Methods Plant materials The experimental population consists of 204 seedling genotypes. The scions were trees from a source population growing at the University of California, Riverside, Agricultural Operations (AgOps) and represented the

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John A. Menge, Greg W. Douhan, Brandon McKee, Elinor Pond, Gary S. Bender and Ben Faber

losses of over $40 million ( Coffey, 1992 ). The use of tolerant rootstocks to control Phytophthora root rot (PRR) of avocado has long been proposed as the ultimate method for managing the disease ( Zentmyer, 1957 , 1963 ) and research on developing PRR

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Rayane Barcelos Bisi, Rafael Pio, Daniela da Hora Farias, Guilherme Locatelli, Caio Morais de Alcântara Barbosa and Welison Andrade Pereira

recommended for commercial crops. Thus, identifying compatibility between cultivars is of economic importance because partial compatibility may reduce yield ( Goldway et al., 1999 ; Tatari et al., 2017 ). Several methods can be applied to the study of the

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Xiucai Fan, Renzong Zhao, Qianqian Wang, Chonghuai Liu and Jinggui Fang

content and higher monomer content, and acquire information for future color breeding programs in grapes. Materials and Methods Plant materials. Forty-nine varieties were analyzed from the Kyoho grapevine series ( Table 1 ). Young leaves and ripe berries