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Scott D. Haley, Phillip N. Miklas, Lucia Afanador, and James D. Kelly

The objective of this study was to evaluate the degree of RAPD marker variability between and within commercially productive market classes representative of the Andean and Middle American gene pools of common bean (Phaseolus vulgaris L.). Six sets of near-isogenic lines were screened with oligonucleotide primers in the polymerase chain reaction-based RAPD assay. Simultaneous analyses with at least three sets of lines enabled us to score RAPD markers between the two major gene pools, races within the same gene pool, and different genotypes of the same race (within race). A “three-tiered” pattern of polymorphism was observed: between gene pools> between races> within races. The overall level of polymorphism between the Andean and Middle American gene pools was 83.4%. The overall level of polymorphism between races within the same gene pool was similar for Andean races (60.4%) and Middle American races (61.7%). The level of polymorphism between related commercial navy bean lines was 39.2% and between related commercial snap bean lines was 53.6 %. The inherent simplicity and efficiency of RAPD analyses, coupled with the number of polymorphisms detectable between related commercial genotypes, should facilitate the construction of RAPD-based genetic linkage maps in the context of populations representative of most bean breeding programs.

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Min Wang, Wenrui Liu, Biao Jiang, Qingwu Peng, Xiaoming He, Zhaojun Liang, and Yu’e Lin

heat-sensitive pools in cucumber. The genome-wide SNP analysis allowed us to detect a genomic region that harbored the target gene involved in HS. Genetic analysis and fine-mapping of the HS gene were performed to provide a basis for molecular cloning

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Marko Maras, Barbara Pipan, Jelka Šuštar-Vozlič, Vida Todorović, Gordana Đurić, Mirjana Vasić, Suzana Kratovalieva, Afrodita Ibusoska, Rukie Agić, Zdravko Matotan, Tihomir Čupić, and Vladimir Meglič

gene pools. A prevalence of the Andean “C” and “T” phaseolin types (76%) was first detected by Gepts and Bliss (1988) , and was then confirmed by Lioi (1989) in an analysis of a large collection from Italy, Greece, and Cyprus (66% in total), by

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Marko Maras, Jelka Šuštar-Vozlič, Wolfgang Kainz, and Vladimir Meglič

Common bean is by far the most widely consumed grain legume in the world ( Singh, 2001 ). Domestication of common bean took place in two, already diverged ancestral wild gene pools distributed from northern Mexico to Colombia (Mesoamerican gene pool

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Hisayo Yamane, Yukinobu Kashiwa, Tomomi Ooka, Ryutaro Tao, and Keizo Yonemori

endodormancy, we generated two subtraction libraries. First, we generated a SSH/MOS library containing gene pools that are expressed preferentially in endodormant buds compared with ecodormant buds to search for genes that are down-regulated by endodormancy

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Gayle M. Volk, Adam D. Henk, Christopher M. Richards, Philip L. Forsline, and C. Thomas Chao

. niedzwetzkyana . We use genotypic and phenotypic data to determine if the gene pools in the NPGS that comprise these taxa can be distinguished from one another. Materials and Methods Malus sieversii and several accessions of M. sieversii var. kirghisorum

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Yu Zong, Ping Sun, Xiaoyan Yue, Qingfeng Niu, and Yuanwen Teng

-adjusted metric of allelic richness ( A r ) was calculated using FSTAT 2.9.3 ( Goudet, 1995 ). Allele richness was standardized for 11 individuals. Genetic differentiation among populations. The number of homogeneous gene pools ( K ) was evaluated using a Bayesian

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Júlia Halász, Andrzej Pedryc, Sezai Ercisli, Kadir Ugurtan Yilmaz, and Attila Hegedűs

-incompatibility controlled by a single locus with multiple variants, termed S -haplotypes ( de Nettancourt, 2001 ). The S -haplotype contains a gene ( S - RNase ) encoding for a ribonuclease enzyme ( McClure et al., 1989 ), and the recently identified S - haplotype

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Zhengwang Jiang, Feiyan Tang, Hongwen Huang, Hongju Hu, and Qiliang Chen

the modern Chinese fruit industry over the past three decades. There has been large-scale cultivation of a few elite cultivars and top-grafting or replacement of old cultivars or landraces. This genetic loss could lead to serious erosion of the gene

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Kim E. Hummer

. Many countries in both the northern and southern hemispheres are producing these healthful fruits for local use and also for export to population centers. The use of diverse species in breeding programs has broadened gene pools and has been the