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5S ribosomal DNA (rDNA) was visualized on the somatic metaphase chromosome of persimmon (Diospyros kaki) and ten wild Diospyros species by fluorescent in situ hybridization (FISH). The digoxigenin (DIG)-labeled 5S rDNA probe was hybridized onto the chromosomes and visualized by incubation with anti-DIG-fluorescein isothiocyanate (FITC). Strong signals of 5S rDNA probe were observed on several chromosomes of Diospyros species tested. Furthermore, multicolor FISH using 5S and 45S rDNA probes differently labeled with DIG and biotin, revealed separate localization of the two rDNA genes on different chromosomes of Diospyros species tested, suggesting that 5S and 45S rDNA sites can be used as chromosome markers in Diospyros. The number of 5S rDNA sites varied with the Diospyros species. More 5S rDNA sites were observed in four diploid species native to Southern Africa than in three Asian diploid species. The former had four or six 5S rDNA sites while the latter had two. Three Asian polyploidy species had four to eight 5S rDNA sites. Among the Asian species, the number of 5S rDNA sites seemed to increase according to ploidy level of species. These features of 5S rDNA sites were very similar to those of 45S rDNA sites in Diospyros. Phylogenetic relationship between D. kaki and wild species tested are discussed based on the number and chromosomal distribution of 5S and 45S rDNA.

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ribosomal RNA gene families (18S-28S rDNA and 5S rDNA) using fluorescent in situ hybridization (FISH). Fig. 1. Mature Christia obcordata plants grown under greenhouse environment at the U.S. Department of Agriculture, Agriculture Research Service unit in

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(references in Chung et al., 2008 ) [e.g., Phaseolus vulgaris ( Pedrosa-Harand et al., 2006 ) and certain Oryza species ( Chung et al., 2008 )]. The fluorescent in situ hybridization (FISH) technique allows physical mapping of rDNA locus regardless of

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, it is unlikely that H. syriacus originated from a recent autopolyploid event, as four 5S rDNA signals would have been expected. Fig. 4. Fluorescent in situ hybridization (FISH) analysis of metaphase, root tip chromosomes in a ploidy series of

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) . Fluorescent in situ hybridization. The slides were prepared as for the C-banding analysis. The cloned 45S rDNA from rice ( Oryza sativa L.) was labeled with fluorescein-12-dUTP by nick translation. To label the probe, a 50-μL labeling mixture containing 5 μL

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may also be homologous to other citrus species. Chromosome regions, which are homologous among species, possibly contain highly conserved sites such as rDNA. The rDNA fluorescent in situ hybridization (FISH) signals in Meiwa kumquat ( Fortunella

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. To conserve the aforementioned genetic resource, the main ex situ collection in the country was established in the fruit tree nursery Srebrenik (Spionica, B&H, lat. 44°45′45″N, long. 18°29′49″E) in 2000. Aside from local traditional B&H cultivars, the

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isolation and fluorescent in situ hybridization data in plants J. Expt. Bot. 54 11 23 Singh, R.J. 2003 Plant cytogenetics 2nd ed CRC Press Boca Raton, FL 10.1111/j.1439-0523.1987.tb01111.x Singh, R

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, delicate corymbs of white flowers in early summer ( Dirr, 1998 ). ‘Annabelle’ is a cold-tolerant smooth hydrangea cultivar that can survive in Beijing (hardiness zone 7) and other cities in northern China (zones 6 and 7). Hybridization between distantly

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managing Malus ex situ germplasm collections as well as for collection and preservation strategies of these genetic resources ( Hokansson et al., 1998 ). Characterizing accessions maintained in ex situ apple collections, using microsatellite markers, has

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