Treating `Elliott's White' cut carnations with 50 or 100 mm aminotriazole for 4 days inhibits the respiratory climacteric and significantly extends vase life. Aminotriazole induced time- and concentration-dependent inhibition of ethylene evolution and onset of the ethylene climacteric by inhibiting ACC synthase activity. Flowers treated with 50 or 100 mm aminotriazole for 2 days exhibited concentration-dependent increases in ethylene evolution, respiratory activity, ACC synthase activity, and petal ACC content in response to the application of exogenous ethylene at 10 μl·liter-1. Senescence-associated morphological changes, increased ACC synthase activity, ACC content, and ethylene evolution were completely inhibited in flowers treated for 4 days with 100 mm aminotriazole. Although treatment with 50 mm aminotriazole for 4 days did not completely inhibit components of the ethylene biosynthetic pathway, no morphological or respiratory responses to the application of exogenous ethylene at 10 μl·liter-1 were observed, a result indicating that prolonged aminotriazole treatment inhibited ethylene action. Chemical names used: 3-1H-amino-1,2,4-triazole-1-yl (aminotriazole), 1-aminocyclopropane-1-carboxylic acid (ACC).
Steven A. Altman and Theophanes Solomos
Wen-Quan Sun and Nina L. Bassuk
Single-node `Royalty' rose (Rosa hybrida L.) cuttings were used to examine the relationship between adventitious root formation, budbreak, and ethylene synthesis following IBA treatment. IBA was applied as a lo-second basal quick dip before rooting, and AIB, GA3, STS, and ethephon were applied either as basal dips or foliar sprays. IBA application increased rooting and inhibited budbreak of cuttings. IBA 2 600 mg·liter-1 greatly inhibited budbreak during 4 weeks of rooting. IBA treatment stimulated ethylene synthesis, which was inversely correlated with budbreak of cuttings. Ethephon also significantly inhibited budbreak. Budbreak of rose cuttings was completely prevented by repeated ethephon sprays used to maintain high endogenous ethylene levels during the first 10 days. Treatment with STS, an ethylene-action inhibitor, improved budbreak. The inhibition of budbreak by IBA treatment resulted primarily from elevated ethylene levels. Root initiation and root elongation of cuttings initially inhibited budbreak, but later promoted budbreak. Chemical names used: indole-3-butyric acid (IBA); gibberellic acid (GA3); silver thiosulfate (STS); AIB, aminoisobutyric acid (AIB); (2-chloroethyl)-phosphoric acid (ethephon).
Xingbin Xie, Todd Einhorn, and Yan Wang
texture J. Expt. Bot. 56 2029 2036 Franck, C. Lammertyn, J. Tri Ho, Q. Verboven, P. Verlinden, B. Nicolai, B.M. 2007 Browning disorders in pear fruit Postharvest Biol. Technol. 43 1 13 Gapper, N.E. Bai, J. Whitaker, B.D. 2006 Inhibition of ethylene
Xingbin Xie, Congbing Fang, and Yan Wang
409 Xie, X. Einhorn, T. Wang, Y. 2015 Inhibition of ethylene biosynthesis and associated gene expression by aminothoxyvinylglycine and 1-methylcyclopropene and their consequences on eating quality and internal browning of ‘Starkrimson’ pears J. Amer
Nancy Santana-Buzzy, Adriana Canto-Flick, Lourdes G. Iglesias-Andreu, María del C. Montalvo-Peniche, Guadalupe López-Puc, and Felipe Barahona-Pérez
The in vitro production of ethylene and its effects on the development of Habanero pepper (Capsicum chinense Jacq.) plantlets were evaluated using nonventilated containers (NVCs) and ventilated containers (VCs). Shoots of Habanero pepper between 0.5 and 1.0 cm of height were cultivated in Magenta culture boxes and samples of the headspace atmosphere were taken every four days during the previously established culturing time of 40 days. The presence of ethylene was detected in the NVCs and produced a negative effect on the development of plantlets. In a second phase of this work, the effect of silver nitrate (AgNO3) and cobalt chloride (CoCl2) on ethylene production was evaluated during in vitro development of Habanero pepper plantlets. Concentrations of 50, 300, and 500 μm of each ethylene inhibitor were used in the culture medium. Although cobalt chloride partially inhibited the production of ethylene during in vitro culture of this species, at low concentrations the plantlets presented some degree of vitrification and the highest concentration proved to be toxic for the plantlets. Silver nitrate added to the culture medium did not inhibit ethylene production, however, it did inhibit the effect of this hormone on the plantlets. In fact, when high concentrations of silver nitrate were used (300 μm), high amounts of ethylene were detected in the headspace of the vessels and plantlets were actually healthier.
James R. Gorny and Adel A. Kader
Ethylene biosynthesis of Golden Delicious apple fruit at 20°C is rapidly inhibited by a controlled atmosphere of air + 20% CO2. However, in vitro ACC oxidase activity and ACC content were not significantly different between air and air + 20% CO2 treated fruit, To determine the in vivo effects of CO2 treatment, both in vivo and in vitro enzyme activity essays were performed in en atmosphere of air or air + 20% CO2. Western blots were also performed to quantify the amount of ACC oxidase protein present in the air and air + 20% CO2 treated fruit.
We believe that in vivo cytosolic pH changes, induced by CO2, may reduce the in vivo catalytic capacity of ACC oxidase, end hence significantly reduce ethylene biosynthesis in climacteric tissue,
Yusuke Kubo, Shinobu Satoh, Haruka Suzuki, Toshinori Kinoshita, and Nobuyoshi Nakajima
ethylene ( Fig. 3 ; Table 1 ). Thus, the inhibition of endogenous ethylene reception reduced cotyledon curling. Exogenously applied ethylene promoted cotyledon curling. To investigate whether ethylene treatment causes cotyledon curling, we injected
Greg McCollum and Pilar Maul
role in citrus fruit physiology. First is the effect of exogenous ethylene on wound-induced ethylene. Exposure of citrus peel discs to ethylene results in the inhibition of wound-induced ethylene, the result of a suppression of ACC, the immediate
Michael S. Reid and Fisun G. Çelikel
. The ethylene response of carnation petals at 20 °C was completely inhibited when they had previously been treated for 6 h at 20 °C with 1 nL·L −1 1-MCP ( Fig. 2 ). When the 1-MCP treatment temperate was 0 °C, there was almost no inhibition of the
Ting-Ting Li, Zhi-Rong Li, Kang-Di Hu, Lan-Ying Hu, Xiao-Yan Chen, Yan-Hong Li, Ying Yang, Feng Yang, and Hua Zhang
concerned about its appearance and textual quality. As a typical climacteric fruit, kiwifruit postharvest ripening and senescence are very sensitive to ethylene, and even extremely low concentrations (0.1 μL·L −1 ) can stimulate fruit ripening and softening