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locations in central and western North Carolina were evaluated to determine the effects of a warm production climate and high tunnel cultivation on anthocyanin, carotenoid, tocopherol, and ellagitannin content, among a number of other fruit quality factors

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subsp. michiganensis , Pseudomonas syringae pv. tomato , Xanthomonas fragariae ) and to contain a galloyl-bis-HHDP-glucose isomer, an ellagitannin known for antibacterial activity. Materials and methods Bacteria. Xanthomonas fragariae

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polyphenols, called hydrolysable tannins, that have relatively newly discovered human health benefits ( Cerda et al., 2005 ; Ross et al., 2007 ; Seeram et al., 2007 ; Viljanen et al., 2004 ), include ellagitannins, which are also present in red raspberries

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date sample to measure nutraceuticals including ORAC and levels of total phenolics, total anthocyanins, total ellagitannins, total flavonols, and resveratrol by methods previously described in Cho et al. (2004 , 2005) , Hager et al. (2008) , and

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was repeated with methanol/water/formic acid (60:37:3 v/v/v). The filtrates were adjusted to a final volume of 250 mL with extraction solvent to assure complete extraction of the nutraceutical compounds. Ellagitannins and flavonols. Sample extracts (3

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were adjusted to a final volume of 200 mL using a mix of the previous extraction solvents (50:50 v/v) and stored at −80 °C until analysis. Ellagitannins and flavonols. Sample extracts (3 mL) were dried using a Speed Vac concentrator (ThermoSavant

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2 h. Absorbance was measured at 760 nm with results expressed as milligrams of gallic acid equivalents per 100 g fresh weight. Anthocyanin, ellagitannin, flavonol, and resveratrol analysis. For anthocyanin analysis, subsamples (5 mL) of extract

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compounds such as phenolic acids and flavonoids, including flavonols and anthocyanins. Important phenolic acids in strawberries are the ellagitannins and ellagic acid glucosides, which break down to pure ellagic acid, also present in the fruit ( Cerdá et

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anthocyanin by high performance liquid chromatography [HPLC (Shimadzu, Portland, OR)] in a manner similar to that described by Connor et al. (2005) . Total ellagitannin determined by HPLC was quantified using purified Sanguiin H6 as a standard. Vitamin C was

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(Shimadzu, Portland, OR)] in a manner similar to that described by Connor et al. (2005) . Total ellagitannin determined by HPLC was quantified using purified Sanguiin H6 as a standard. Vitamin C was measured as part of the polyphenol HPLC method. It had a

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