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E. Vanessa Campoverde, Georgina Sanahuja, and Aaron J. Palmateer

are considered stress factors affecting the incidence of many crop diseases ( Schoeneweiss, 1975 ). Heavy rainfall and severe weather conditions are known to provide a window of opportunity for diseases caused by oomycetes such as Pythium and

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Beth Clendenen, B.K. Behe, and K.L. Bowen

Eleven rose cultivars were field planted and evaluated weekly for disease, defoliation, and overall vigor in order to compare natural resistance to blackspot (Diplocarpon rosae). Alternative treatments were also compared for efficacy in low-maintenance disease control. Treatments included a bimonthly application of chlorothalonil, a bimonthly application of a horticultural oil, an application of chlorothalonil based on rain events, and a no-treatment control. Cultivars showed significant differences in disease severity, defoliation, and overall performance, with old garden rose varieties showing more natural disease resistance than modern susceptible varieties included in the study. Chlorothalonil applied on a 14-day spray schedule did provide a significant decrease in blackspot disease severity when compared to other treatments. A significant incidence of secondary disease including Cercospora rosicola and Botrytis cinerea occurred on old garden rose varieties. No treatment differences were found for these diseases. `Belinda's Dream', `The Fairy', and `Red Mediland' ranked highest in overall performance throughout the season.

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Daniel P. Gillespie, Chieri Kubota, and Sally A. Miller

understanding of hydroponic nutrient availability at varied pH. The results of Expt. 2 indicate that growing basil in nutrient solution pH 4.0 can effectively reduce P. aphanidermatum disease incidence and severity after zoospore inoculation without

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Karen L.B. Gast

Fresh-cut peonies are one of few cut flowers that can be stored for weeks and still provide a marketable flower. Peonies are usually marketed by color: reds, pinks, whites, and corals. Several different cultivars may be included in each color depending on their country of origin and time during the harvest season. Previous work with peonies has shown that different cultivars of the same color may behave differently during postharvest handling, whether it is storage life, vase life, opening time, storage temperature, etc. One problem of long-term cold storage is diseases that may render flowers unmarketable. This study evaluated the effect of four storage disease prevention treatments on seven peony cultivars, two reds, two pinks, and three whites, stored at 1 °C. The four disease prevention treatments included a control, methyl jasmonate during storage, a pre-storage calcium chloride pulsing for 2 h at room temperature, and a pre-storage fungicide spray. Flowers were evaluated for disease incidence on leaves and flowers, and for flower bud openness after 4, 8, and 12 weeks of cold storage. Overall results support previous work that shows peony cultivars react differently to postharvest treatments. Two cultivars were greatly affected by the disease prevention treatments and three were moderately affected, while there were few treatment effects seen with the other two. The calcium chloride pulse produced the greatest disease incidence and resulted in the flowers being more opening, which is not desirable. There was often no difference in the control, methyl jasmonate, and fungicide treatments. It appears that pre-storage treatments may not be beneficial for some fresh-cut peony cultivars.

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Jennifer Green, Derald A. Harp, and Kevin L. Ong

Phytophthora diseases are economically important, requiring the use of chemical fungicides and, more recently, biological controls. Recent research suggests that composted bark products may lessen the impact of the disease, even in the absence of these chemicals. An experiment was conducted to compare chemical and biological fungicides to untreated pine bark compost. Impatiens wallerana plugs were transplanted from 288 trays into 1801 trays. All plants were planted into Berger BM-7, 35% composted bark mix (Berger Horticulture, Quebec, Canada). Media was prepared by premixing one of the five following fungicide treatments: 1) Control, 2) Banrot at 0.6 g/L, 3) Root Shield at 1.6 g/L, 4) Actino-Fe at 5.1 g/Ll, or 5) SoilGard at 1.6 g/L. Plants received no fertilizer. Three strains of Phytophthora were grown in 25 °C on clarified V8 media. Pathogenic inoculum was made by macerating the growth media and fungi in 100 ml H2O. Mixture was pulse-blended for 1 min, and an additional 200 mL dH2O was added. Inoculation was 5 ml per plant. Flats were kept on a misting bench, and misted twice daily for 15 min. The experiment was set up using a RBD repeated six times with three plants per rep. Plants were rated weekly for 5 weeks using a damage scale of 0 to 5, with 0 indicating no sign of disease and 5 being dead. Statistical analysis was conducted using a Chi-Square. Disease incidence between the biological, chemical, and composted bark treatments did not differ, with all treatments providing complete control. At least in this study, the use of composted pine bark media provided Phytophthora control equivalent to current chemical and biological fungicides.

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Christopher S. Cramer, Neel Kamal, and Narinder Singh

plant. Materials and Methods Eighteen commercial cultivars, three NMSU cultivars, and 17 NMSU experimental breeding lines were evaluated for IYS disease symptom severity and incidence, thrips number per plant, and leaf color and glossiness when they were

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V.M. Russo and J.C. Díaz-Pérez

Heat stress can limit yield in pepper (Capsicum spp.), generally through flower and fruit abortion. A kaolin-based particle film, originally developed to protect fruit trees from insects, has been found to reduce temperatures in tissues of plants. A kaolin-based particle film was tested to determine if it could be used to improve yields of pepper in Oklahoma and Georgia. In Oklahoma, seedlings of a bell pepper, `Jupiter', and a nonpungent jalapeño, `Pace 103', were transplanted at three progressively warmer planting dates from mid-May to mid-July 2002 and 2003, that would ensure that inflorescences would be subject to high day and night temperatures and treated with the kaolin-based particle film. Applications were begun as the first flowers were set and continued through the settings of the first three flushes of flowers on a three-times a week schedule, or on an as needed basis, to determine if the kaolin-based particle film improved yield. In Georgia, the bell peppers `Camelot' and `Heritage VR' were transplanted on 24 Apr. 2003, and treated with the kaolin-based particle film. In addition to yield, physiological measurements and disease incidences were recorded in Georgia. In both locations treatment with water only served as controls. In Georgia, the kaolin-based particle film had no significant effect on net photosynthesis, stomatal conductance, leaf transpiration or leaf temperature, as measured at midday on clear days. In Oklahoma, planting bell pepper after 15 May is not recommended. Planting the nonpungent jalapeño after mid-June can reduce yields. The kaolin-based particle film did not affect yield at either location and is not recommended for use on peppers.

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Kaori Ando and Rebecca Grumet

Fruit rot induced by Phytophthora capsici Leonian is an increasingly serious disease affecting pickling cucumber (Cucumis sativus L.) production in many parts of the United States. The absence of genetically resistant cultivars and rapid development of fungicide resistance makes it imperative to develop integrated disease management strategies. Cucumber fruit which come in direct contact with the soil-borne pathogen are usually located under the canopy where moist and warm conditions favor disease development. We sought to examine whether variations in plant architecture traits that influence canopy structure or fruit contact with the soil could make conditions less favorable for disease development. As an extreme test for whether an altered canopy could facilitate P. capsici control, we tested the effect of increased row spacing and trellis culture on disease occurrence in the pickling cucumber `Vlaspik'. Temperature under the canopy was lowest in trellis plots, intermediate in increased spacing plots, and highest in control plots. Disease occurrence in the trellis plots was significantly lower than in other treatments, indicating that preventing fruit contact with the soil reduced disease occurrence. The effect of currently available variation in plant architecture was tested using nearly-isogenic genotypes varying for indeterminate (De), determinate (de), standard leaf (LL), and little leaf (ll) traits. Plants with standard architecture had higher peak mid-day temperatures under the canopy and greater levels of P. capsici infection; however, levels of disease occurrence were high for all genotypes. Screening a collection of ≈150 diverse cucumber accessions identified to serve as a representative sample of the germplasm, revealed variation for an array of architectural traits including main stem length, internode length, leaf length and width, and number of branches; values for `Vlaspik' were in the middle of the distribution. Plant architectures that may allow for more open canopies, including reduced branching habit and compact growth, were tested for disease incidence. One of the compact lines (PI 308916), which had a tendency to hold young fruit off the ground, exhibited lower disease occurrence. The reduced disease occurrence was not due to genetic resistance, suggesting that architecture which allows less contact of fruit with the soil could be useful for P. capsici control for pickling cucumber.

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E. Fava, D. Janik, C. Madramootoo, and K.A. Stewart

31 ORAL SESSION 4 (Abstr. 024–033) Crop Production–Diseases and Insects

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Nader Soltani, George Lazarovits, and Arran Brown

Hungavit® products contain extracts of earthworm castings and are marketed by BioLife Ltd. as liquid Bio-leaf fertilizer and plant conditioner. Three experimental plots were set up on the AAFC-SCPFRC research farm to evaluate Hungavit®UR, Hungavit®P, and a preparation containing the equivalent amount of N, P, K fertilizer. In Spring 1998, four replicate plots/treatment of tomato, pepper, and potato were set up in a randomized block design. Each plot received the following treatments: untreated control, Hungavit®UR for tomato and pepper or Hungavit®P for potato, and the fertilizer equivalent of Hungavit®UR or P without the organic components. Tomato and pepper plants were treated three times by foliar application at the rate of 5 L/ha using 300 L of water/ha carrier. Leaf chlorophyll contents were measured at 2, 4, and 6 weeks after initial treatment application. Early and total yield were determined. Tomato fruit were evaluated for symptoms of bacterial spot, early blight, anthracnose, and blossom end rot; pepper fruit for bacterial spot; and potato tubers for potato scab. Both Hungavit® and its equivalent fertilizer application increased the chlorophyll readings significantly in at least one measurement for tomato, pepper, and potato plants. Although there were 40% to 55% fewer diseased tomato and pepper fruit in fertilizer and Hungavit® UR treatments, this was not statistically significant from the control treatments. Fertilizer treatment also reduced scab incidence in tubers by 50%, but the overall scab level was very low even in untreated plots. Hungavit® and its fertilizer equivalent had no significant effect on the early or total yield of tomato, pepper, or potato plants.