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Yonghong Guo, Richard T. Olsen, Matthew Kramer, and Margaret Pooler

plants has been reported ( Ganci et al., 2013 ), but it requires space for multiple replications of large containerized plants or in-ground assays with obvious disease containment issues to manage. The objective of this study was to develop simple and

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David Noshad, Andrew Riseman, and Zamir Punja

regiment included yearly top-dressing with Osmocote 18-6-12 at 5 g/1-gal pot. Fungicides were not used during stock production because we had very little disease pressure and we did not want to risk crosscontamination affecting the in vivo assay. Table

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Binoy Babu, Gary Knox, Mathews L. Paret, and Francisco M. Ochoa-Corona

, specific, and sensitive detection assay for either detection or confirmation of the rose rosette disease. Rose rosette emaravirus is a recently characterized virus and little is known about its diversity and biology. Currently, neither enzyme

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Alejandro Martínez-Bilbao, Amaya Ortiz-Barredo, Emilio Montesinos, and Jesús Murillo

leaf assay; additionally, it is still possible that the germplasm collection is enriched in cultivars that have an increased resistance to diseases. Because of the limitations for the manipulation of E. amylovora in Spain, and to speed up the

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Mohsen Mohseni-Moghadam, Christopher S. Cramer, Robert L. Steiner, and Rebecca Creamer

addition, samples were tested by ELISA to determine a correlation between the disease rating and absorbance value. Enzyme-linked immunosorbent assay. The assay was performed according to Copeland (1998) with some minor adjustments. Each sample was divided

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Lisa A. Beirn, William A. Meyer, Bruce B. Clarke, and Jo Anne Crouch

Rust ( Puccinia sp.) is a common fungal disease of kentucky bluegrass (KBG; Poa pratensis L.), one of the most commonly used cool-season turfgrasses on athletic fields, recreation areas, sod farms, and residential lawns. Diseased turf typically

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John L. Coffey, Alvin M. Simmons, B. Merle Shepard, Yaakov Tadmor, and Amnon Levi

Watermelon cultivars [ Citrullus lanatus (Thunberg) Matsum. and Nakai var. lanatus ] share a narrow genetic base ( Levi et al., 2001a ) and are susceptible to a large number of pests and diseases including whiteflies (Hemiptera: Aleyrodidae

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Weining Wang, Yanhong He, Zhe Cao, and Zhanao Deng

lower IDM disease severity from 6 to 10 dpi and lower sporangia densities at 8 and 10 dpi, compared with diploids. A significant reduction of IDM disease incidence was also observed in one of the tetraploids (4x_3) in in vivo inoculation assays

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Rui Li, Lu Fan, Jingdong Lin, Mingyang Li, Daofeng Liu, and Shunzhao Sui

assays. In our study, detached leaves were inoculated with a bacterial pathogen to identify disease resistance—an approach that has been used frequently in previous work ( Chawla and Wenzel, 1987 ; Ge et al., 2015 ; Xu et al., 2007 ). Three regenerated

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Parminder S. Multani, Christopher S. Cramer, Robert L. Steiner, and Rebecca Creamer

controlling onion thrips is with insecticides, which has become more challenging as a result of the development of insecticidal resistance ( Allen et al., 2005 ; Shelton et al., 2003 ). Plants under disease, salt, nutrient, water, temperature, or other stress