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recent years, however, the U.S. citrus industry has suffered major production losses due to citrus huanglongbing (HLB), or greening disease. The disease is caused by the bacterium Candidatus Liberibacter asiaticus ( C Las), and it is transmitted by an

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Citrus greening (HLB) has emerged as the most significant disease in citrus ( Citrus sp.) agriculture ( Bové, 2006 ). The disease is associated with the phloem-limited fastidious gram-negative α-proteobacteria, Candidatus Liberibacter species

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, because of phytosanitary issues in Mexico, such as sweet orange scab ( Elsinoë australis ) and citrus greening ( FreshFruitPortal.com, 2011 ). Further stoking the interest of prospective U.S. producers are results of a demonstration plot of tahiti limes at

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diseases and can enhance their horticultural attributes, such as size, yield, and fruit quality ( Bowman et al., 2016 ; Wutscher and Bowman, 1999 ; Wutscher and Hill, 1995 ). Since the outbreak of citrus greening or Huanglongbing (HLB), research of

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, hurricanes, pest pressures, and diseases such as citrus canker and citrus greening [huanglongbing (HLB)] ( Rossi et al. 2021 ; Singerman and Rogers 2020 ; Vashisth and Kadyampakeni 2020). As a result, during the past 20 years, citrus production in Florida

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or citrus greening disease ( Halbert and Manjunath, 2004 ). Nymphs are considered more efficient at acquisition and adults at the transmission and spread of these pathogens. Feeding damage by the larvae of P. citrella exacerbates the spread of

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The asian citrus psyllid is a detrimental pest to citrus crops when it serves as a vector of the pathogen that causes greening [huanglongbing (HLB)]. Transmission of this disease causes mottling, chlorosis, dieback, and reductions in fruit size and

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Florida has long been associated with citrus ( Citrus sp.), which had a total production value of more than $800 million in 2019. However, since the first detection of HLB or citrus greening disease [ Candidatus Liberibacter asiaticus ( C Las

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development of eight new pGreen-derived GFP vectors and preliminary studies to evaluate one of the vectors using citrus Agrobacterium -mediated transformation. Materials and Methods pGreen vectors and green fluorescent protein cassettes. Three

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deoxycholate pre-treatment for selective quantification of infectious viruses in drinking water samples Food Environ. Virol. 11 40 51 https://doi.org/10.1007/s12560-01368-2 Capoor, S.P. Rao, D.G. Vishwanath, S.M. 1974 Greening disease of citrus in

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