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Hong Lin and M. Andrew Walker

The DNA extracted from cambium tissues of grape (Vitis spp., Muscadinia rotundifolia Small) rootstocks was found to be suitable for molecular analysis. Its quality was equivalent to that of DNA extracted from leaf tissues, although the yield was higher from leaves. The use of cambium tissue allows DNA extractions during dormancy or from grafted rootstocks where leaves are not available. The DNA extracted was suitable for restriction enzyme digestion and for analysis by restriction fragment length polymorphism (RFLP), randomly amplified polymorphic DNA (RAPD), and simple sequence repeats.

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Olfa Zarrouk, Pilar S. Testillano, María Carmen Risueño, María Ángeles Moreno and Yolanda Gogorcena

of the newly formed cambium causes an invagination of the cambial zone, a differentiation of parenchymatous tissue in the place of xylem ( Deloire and Hébant, 1983 ; Gur et al., 1968 ), and a lack of lignification of cells interlocked at the graft

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Hui-qing Li, Qing-he Li, Lei Xing, Gao-jie Sun and Xiu-lian Zhao

Swanson, 1990 ). The objectives of this study were 1) to compare cold hardiness of the cambium, phloem, xylem, and pith for woody species by TTC staining and TTCLT 50 ; and 2) to compare cold hardiness estimations of woody species by ELLT 50 and TTCLT 50

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Renae E. Moran, Bryan J. Peterson, Gennaro Fazio and John Cline

assessing injury in different tissues, but the level of browning that leads to mortality may differ between the xylem, cambium, and phloem. Acclimation begins in distal parts of the tree and progresses toward the base, so the trunk is the last part of the

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Naveen Kumar, Fnu Kiran and Ed Etxeberria

Swingle citrumelo rootstock. ( A ) Close up of phloem tissue from control Huanglongbing (HLB)-free roots. ( B ) Close up of phloem tissue of HLB-infected roots. X = xylem; P = phloem; Co = cortex; Ca = cambium; SE = sieve element; CC = companion cell. Fig

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Arthur Q. Villordon, Don R. La Bonte, Nurit Firon, Yanir Kfir, Etan Pressman and Amnon Schwartz

cambium development and lignification determined final storage root count and root yield. It is the combination of a contiguous, active regular vascular cambium and proliferation and growth of anomalous cambia that enabled storage roots to add girth

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Michele R. Warmund, Billy G. Cumbie and Mark V. Coggeshall

, and oak trees. In cross-sections of 1-year-old Chinese chestnut stems, the vascular cambium was arranged in a regular five-lobed cylinder in which lobes were aligned with nodes ( Fig. 1 ). Primary phloem fibers in a discontinuous ring were observed and

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Craig Brodersen, Cody Narciso, Mary Reed and Ed Etxeberria

outside ( Figs. 2A and 3A ). The newly produced metaphloem ( Fig. 2A , black arrow) appears as a layer of thin-walled cells between the cambium and the protophloem ( Fig. 2A , white arrow). Differentiation of the metaphloem outwardly becomes evident with

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Fan Cao, Xinwang Wang, Zhuangzhuang Liu, Yongrong Li and Fangren Peng

from outside to inside was recognized as periderm, cortex, and secondary vascular tissue and pith, respectively ( Fig. 3A ). Periderm, which consists of the skin pore structure, is composed of a cork layer, cork cambium, and phelloderm. Secondary

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Whitney J. Garton, Mark Mazzola, Nairanjana Dasgupta, Travis R. Alexander and Carol A. Miles

this tissue. The resulting excisions were 1 to 3 mm beyond the original canker margins, but cambium tissue was present throughout the excision zone. Excised material was removed from the orchard. The CAU treatment was carried out with a self