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Xiaoling Jin, Xijun Hu, Youping Sun, Donglin Zhang and Ping He

per rinse, then in 50% (v/v) commercial bleach for 15 min, and rinsed five or six times (5 min per rinse) with sterile distilled water. The pericarp and testa of the seeds were removed, and the immature embryos were excised as explants for callus

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Sachiko Matsubara and Hegazi H. Hegazi

Callus initiation and growth and plantlet regeneration were studied using eight cultivars of Raphanus sativus L., including six Japanese radishes, one Chinese and one small `Comet' radish. The basal medium was composed of Murashige and Skoog inorganic salts, 2.0 mg myo-inositol/liter, 0.5 mg each of nicotinic acid and pyridoxine·HCl/liter, and 0.1 mg thiamine·HCl/liter, 30 g sucrose and 2 g Gelrite/liter. High callus yields were obtained on basal medium containing (mg·liter-1) 0.1 2,4-D and 1.0 BA for two Japanese radishes and 0.1 NAA and 1.0 kinetin for `Comet' radish. Shoots were regenerated from callus by subculturing on basal medium containing 0.1 or 1.0 mg BA/liter and then transferring to basal medium. Rooting occurred on basal medium. Although callus was obtained in all eight cultivars, shoots and plantlets were regenerated only from `Moriguchi', `Nerima Shirinaga', and `Comet'. Chemical names used: 2-(l-naphthyl) acetic acid (NAA); N-(phenylmethyl)-lH-purine-6-amine (BA); 2,4-dichlorophenoxy acetic acid (2,4-D); 6-(furfurylamino)purine (kinetin).

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June Liu, Zhimin Yang, Weiling Li, Jingjin Yu and Bingru Huang

., 1990 ; Lazar et al., 1988 ). For example, Kendall et al. (1990) showed that plants of winter wheat ( Triticum aestivum ) regenerated from callus and subsequently exposed to below-freezing temperature exhibited significant improvement in cold

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David W. Carey, Mary E. Mason, Paul Bloese and Jennifer L. Koch

callus grafting ( Lagerstedt, 1981 ), which heats the graft union while keeping the rootstock and scion cool, can significantly increase graft success of woody plants ( Avanzato and Tamponi, 1987 ; Lagerstedt, 1984 ). Our objective was to use a hot

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Ya-Long Qin, Xiao-Chun Shu, Wei-Bing Zhuang, Feng Peng and Zhong Wang

eggplant, potato, and tomato. However, compared with plants of the same genus, the callus differentiation frequency (%) of S. torvum is still relatively low, and there is no stable plant regeneration system for the genetic transformation of S. torvum

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Boling Liu, Hongzhou Fang, Chaorong Meng, Ming Chen, Qingdong Chai, Kai Zhang and Shijuan Liu

before pH adjustment and sterilization. All cultures were placed at 24 ± 1 °C under a 14-h photoperiod with 40 μmol·m −2 ·s −1 photosynthetic photon density quipped with cool-white fluorescent lamps (Philips 40 W tubes). Callus induction and propagation

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Piyada Alisha Tantasawat, Atitaya Sorntip and Paniti Pornbungkerd

found that donor plant genotypes and induction media, differing in PGRs, affected both ELS and callus formation. The addition of TDZ and 6-benzylaminopurine (BAP) into the induction medium resulted in the highest percentage of ELS formation ( Tantasawat

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Kelly M. Oates, Darren H. Touchell and Thomas G. Ranney

., 2001 ) as a result of the complexity and number of initial/stem cells in the apical meristem and the potential for each cell to be affected differently. In vitro treatment of organogenic callus can result in fewer chimeric mutations because plants are

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Hua Q. Zhao, Qing H. He, Li L. Song, Mei F. Hou and Zhi G. Zhang

were cut into sections 0.5 cm long. Callus induction and shoot regeneration. The sterilized petioles were cultured on MS basal medium supplemented with different concentrations of NAA (0, 0.01, 0.03, and 0.05 mg·L −1 ), BA (0, 0.1, 0.5, and 1.0 mg·L −1

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Dongliang Qiu, Xiangying Wei, Shufang Fan, Dawei Jian and Jianjun Chen

6-(4-hydroxy-3-methylbut-2-enylamino) purine or ZT and 0.025 μ m IBA in baby food jars. After 10 weeks of culture in a culture room described in the following paragraphs, axillary shoots were used as sources of explants. For producing callus