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Yihui Cui, Peng Zhao, Hongqiang An, Nan Lv, Zifeng Zhang, Wei Pei, and Wanjun Wang

unclear. We have previously established an efficient somatic embryogenesis system in D. candidum , in which new PLBs would be achieved from the embryonic calli ( Zhao et al., 2008 ). In this article, we described the details on the developmental process

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Ruigang Wu, Yi Wang, Ting Wu, Xuefeng Xu, and Zhenhai Han

negative control ( Zheng et al., 2009 ). MdMYB4 overexpression vector construction and apple calli transformation. The complete sequence of the 35S promoter was amplified from the pBI121 vector ( Supplemental Table 2 ) and cloned into the pCAMBIA1304

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Song-jun Zeng, Zhi-lin Chen, Kun-lin Wu, Jian-xia Zhang, Cheng-ke Bai, Jaime A. Teixeira da Silva, and Jun Duan

) Yellowish callus mass developed from a protocorm. ( D ) PLBs developed from a callus mass. ( E ) Combination of shoot clusters, calli, and PLBs. ( F ) PLB-derived seedlings. ( G ) Transplanted seedlings on bark after growing in the greenhouse for 6 months

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Maureen M.M. Fitch, Terryl C.W. Leong, Xiaoling He, Heather R.K. McCafferty, Yun J. Zhu, Paul H. Moore, Dennis Gonsalves, Herb S. Aldwinckle, and Howard J. Atkinson

present work, we report on a quicker, higher-yielding method for transforming anthuriums using embryogenic calli. Additionally, we improved efficiency of transformation of differentiated in vitro-grown tissues. Generation of large quantities of independent

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Chiu-Yueh Hung and Jiahua Xie

regeneration systems. A previous report showed that calli could be induced from the hypocotyls of five Se-hyperaccumulator and three nonaccumulator species of Astragalus by including 13.57 μ m 2,4-dichlorophenoxyacetic acid (2,4-D) in the induction medium

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June Liu, Zhimin Yang, Weiling Li, Jingjin Yu, and Bingru Huang

transformation. The objectives of this study were to establish an effective in vitro culture protocol for generating plants from calli using mature seeds of seashore paspalum and to determine whether in vitro cold selection of somaclonal variations would lead to

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Chandra Thammina, Mingyang He, Litang Lu, Kaishuang Cao, Hao Yu, Yongqin Chen, Liangtao Tian, Junmei Chen, Richard McAvoy, Donna Ellis, Degang Zhao, Yuejin Wang, Xian Zhang, and Yi Li

formation data were recorded at Week 12 of culture and mean number of immature endosperm explants forming calli was calculated based on three replicates for each treatment. Callus induction rate was calculated by dividing the number of explants forming calli

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Li Xu, Suzhen Huang, Yulin Han, and Haiyan Yuan

in growth room at 25 °C in darkness. The frequency of callus and embryogenic callus induction were evaluated 8 weeks after the culture. To increase the frequency of embryogenic callus formation, nonembryogenic calli were subcultured on MS medium

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G.G. Ning and M.Z. Bao

cotyledons of P. mume ( Fig. 1A ) were cultured on a medium that was free from plant growth regulators or contained less than 2.7 μ m NAA or 2.2 or less BA. However, calli ( Fig. 1B ) could be obtained when explants were cultured on half-strength MS

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Akira Sugiura, Yoshiko Matsuda-Habu, Mei Gao, Tomoya Esumi, and Ryutaro Tao

; Tao and Sugiura, 1992b ; Yokoyama and Takeuchi, 1976 ). Shoot tip culture of current-year shoots is possible ( Sugiura et al., 1986 ) as is plant regeneration from calli and cell cultures derived from dormant buds ( Tao et al., 1988 ; Tao and Sugiura