.V. 1991 Identification of markers linked to disease resistance genes by bulked segregant analysis, a rapid method to detect markers in specific genomic regions by using segregating populations Proc. Natl. Acad. Sci. USA 88 9828 9832 Murray, M. Thompson, W
markers associated with traits of interest, permitting the use of marker-assisted selection (MAS) for traits expressed in late development ( Gulsen et al., 2010 ). Bulked segregant analysis (BSA) is a rapid procedure used to efficiently identify markers
. Circumlineatus phenotype with precipitation line indicated by the arrow in representative lines from the t z cl G b v virgarcus BC 3 5-593 × t z sel Cl G b v sellatus BC 3 5-593 population of common bean. AFLP and bulk segregant analysis. DNA was
’, respectively ( Knavel, 1988 , 1990 ; Paris et al., 1984 ). Furthermore, Hwang et al. (2014) identified the dwarf gene mdw1 of the melon ‘PNU-WT1’ dwarf mutant on chromosome 7, which is highly homologous with CKX . Bulked-segregant analysis (BSA) is an
Plant growth habit is an important trait. Our objective was to identify RAPD markers linked to major gene for indeterminate growth habit using bulked segregant analysis in an F2 population from a bean cross Chichara (indeterminate growth habit × PC-50 (determinate growth habit). A total of 132 RAPD primers (600 RAPD primer screened) showed polymorphisms between bulked DNA derived from indeterminate and determinate plants. All markers showed coupling linkage with indeterminate growth habit. RAPD markers of A-8, A-17, C-7, C-15, D-4, D-5, F-6, F-16, G-9, H-3, H-20, and I-7 were 2.2 cM distant from the gene for indeterminate growth habit. Markers of B-7, B-16, B-17, C-8, E-1, F-1, F-20 and H-l9 primers were 4.6 cM distant from the gene for indeterminate growth habit.
Common bacterial blight (CBB), incited by Xanthomonas campestris pv. phaseoli (Xcp), an important disease in common bean (Phaseolus vulgaris L.) Tepary bean (P. acutifolius A. Gray) is of interest to bean breeders because of resistance to CBB. Our objective was to identify RAPD markers linked to major genes for CBB resistance using bulked segregant analysis in an F2 population from a tepary bean cross CIAT640005 (R) X Nebr#4B (S). A total of 57 RAPD primers (602 RAPD primers screened) showed polymorphisms between bulked DNA derived from R and S CBB plants. All markers showed coupling linkage with CBB resistance. A good fit to a 3:1 ratio of bands for presence and absence using 11 RAPD primers was observed in 77 F2 plants. Markers of U-15 and L-7 primers were 2.4 cM distant from the gene for resistance to Xcp strain LB-2. RAPD markers of U-10, U-20, S-12, Y-4, F-13, P-6, Q-1, and Q-ll primers were 2.4 cM distant from the gene for resistance to Xcp strain SC-4A. RAPD markers of IJ-15 and L-7 primers were 8.4 cM distant from the gene for resistance to Xcp strain EKl l. The tepary RAPD linkage group includes three molecular markers and three genes for resistance to Xcp strains EK-l l, LB-2, and SC-4A and spans a length of 19.2 cM. This data supports the presence of Xcp races.
for resistance can also be used to pyramid resistance genes into commercially acceptable cultivars. Amplified fragment length polymorphism (AFLP) markers combined with bulk segregant analysis (BSA) is a powerful technique for identifying markers linked
reps. Disease phenotype was considered resistant when DI was 1.0 or less and susceptible when DI was greater than 1.0. DNA extraction and bulk segregant analysis. Melon genomic DNA was extracted from young leaves using a CTAB method ( Doyle and Doyle
), plants with scores 31–50 as moderately susceptible (MS). Plants were scored as highly susceptible (HS) if they had a score of 51 or higher. DNA extraction and bulked segregant analysis. Total genomic DNA of each F 2 individual was extracted from
to the more poorly resourced pear. This study used SSR markers derived from both pear and apple as well as RAPD markers for bulked segregant analysis (BSA) ( Michelmore et al., 1991 ) across an F 1 population derived from a cross between ‘Aihuali