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Santiago Vilanova, Carlos Romero, Gerardo Llácer, María Luisa Badenes, and Lorenzo Burgos

This report shows the PCR-based identification of the eight known self-(in)compatibility alleles (S 1 to S 7 and S c) of apricot (Prunus armeniaca L.). Two sets of consensus primers, designed from P. armeniaca S-RNase genomic sequences and sweet cherry (P. avium L.) S-RNase-cDNAs, were used to amplify fragments containing the first and the second S-RNase intron, respectively. When the results obtained from the two PCRs were combined, all S-alleles could be distinguished. The identity of the amplified S-alleles was verified by sequencing the first intron and 135 base pairs (bp) of the second exon. The deduced amino acid sequences of these fragments showed the presence of the C1 and C2 Prunus L. S-RNase conserved regions. These results allowed us to confirm S-genotypes previously assigned by stylar ribonuclease analyses and to propose one self-(in)compatibility group (I) and one universal donor group (O) containing unique S-genotypes and self-compatible cultivars (SC). This PCR-based typing system also facilitates the identification of the S c-allele and might be a very useful tool for predicting self-compatibility in apricot breeding progenies.

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Kirk W. Pomper, Anita N. Azarenko, Joel W. Davis, and Shawn A. Mehlenbacher

Random amplified polymorphic DNA (RAPD) markers were identified for self-incompatibility (SI) alleles that will allow marker-assisted selection of desired S-alleles and assist in cloning the locus responsible for the sporophytic SI displayed in hazelnut (Corylus avellana L.). DNA was extracted from young leaves collected from field-planted parents and 27 progeny of the cross OSU 23.017 (S1 S12) × VR6-28 (S2 S26). Screening of 10-base oligonucleotide RAPD primers was performed using bulked segregant analysis. DNA samples from six trees each were pooled into four “bulks,” one for each of the following: S1 S2, S1 S26, S2 S12, and S12 S26. “Super bulks” of twelve trees each for S1, S2, S12, and S26 then were created for each allele by combining the appropriate bulks. The DNA from these four super bulks and also the parents was used as a template in the PCR assays. Amplification products were electrophoresed on 2% agarose gels and photographed under UV light after ethidium bromide staining. 200 primers were screened and one RAPD marker each was identified for alleles S2 (OPI-07700) and S1 (OPJ-141700).

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Ryutaro Tao, Hisayo Yamane, Akira Sugiura, Hideki Murayama, Hidenori Sassa, and Hitoshi Mori

This report identifies S-RNases of sweet cherry (Prunus avium L.) and presents information about cDNA sequences encoding the S-RNases, which leads to the development of a molecular typing system for S-alleles in this fruit tree species. Stylar proteins of sweet cherry were surveyed by two dimensional polyaclylamide gel electrophoresis (2D-PAGE) to identify S-proteins associated with gametophytic self-incompatibility. Glycoprotein spots linked to S-alleles were found in a group of proteins which had Mr and pI similar to those of other rosaceous S-RNases. These glycoproteins were present at highest concentration in the upper segment of the mature style and shared immunological characteristics and N-terminal sequences with those of S-RNases of other plant species. cDNAs encoding these glycoproteins were cloned based on the N-terminal sequences. Genomic DNA and RNA blot analyses and deduced amino acid sequences indicated that the cDNAs encode S-RNases; thus the S-proteins identified by 2D-PAGE are S-RNases. Although S1 to S6-alleles of sweet cherry cultivars could be distinguished from each other with the genomic DNA blot analysis, a much simpler method of PCR-based typing system was developed for the six S-alleles based on the DNA sequence data obtained from the cDNAs encoding S-RNases.

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Rafel Socias i Company, Àngel Fernández i Martí, Ossama Kodad, and José M. Alonso

the ability of a genetically self-compatible cultivar to pollinate itself in the absence of insects ( Weinbaum, 1985 ). Additionally, a good cultivar must always be productive with a crop of good kernel quality. Identification of S alleles was

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Javier Sanzol and Timothy P. Robbins

( Crane and Lewis, 1942 ; Le Lézec, 1998 ; Modlibowska, 1945 ) and insufficient for the systematic identification of S-alleles. Recently, by means of crossing experiments we identified four S-alleles (S 1 –S 4 ) in a group of cultivars on the basis of

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Shawn A. Mehlenbacher

(haplotypes), and the stigmatic surface is the site of the incompatibility reaction ( Thompson, 1979a ). Thompson (1979b) listed the alleles of several cultivars. Additional early work on S-allele identification was reviewed by Germain (1994) . Hampson et

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Beibei Li, Jianfu Jiang, Xiucai Fan, Ying Zhang, Haisheng Sun, Guohai Zhang, and Chonghuai Liu

. Zulini, L. Maul, E. 2004 Development of a standard set of microsatellite reference alleles for identification of grape cultivars Theor. Appl. Genet. 109 1448 1458 This, P. Lacombe, T. Thomas, M.R. 2006 Historical origins and genetic diversity of wine

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Imen Rekik, Amelia Salimonti, Naziha Grati Kamoun, Innocenzo Muzzalupo, Oliver Lepais, Sophie Gerber, Enzo Perri, and Ahmed Rebai

polymerase chain reaction (PCR) and are highly reproducible among different laboratories. Almost all reports of cultivar identification using SSRs assessed differences in lengths of amplified alleles ( Bandelj et al., 2002 , 2004 ; Belaj et al., 2004

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Hafid Achtak, Ahmed Oukabli, Mohammed Ater, Sylvain Santoni, Finn Kjellberg, and Bouchaib Khadari

cultivars or closely related cultivars to establish a molecular identification key. Results Simple sequence repeat polymorphism. The analysis of the 75 fig accessions using 17 SSR loci revealed 62 different genotypes and 85 alleles. The

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María José Arismendi, Patricio Hinrichsen, Ruben Almada, Paula Pimentel, Manuel Pinto, and Boris Sagredo

any single Prunus genotype will have all these attributes. However, it is convenient to incorporate the maximum number of these characteristics to increase its usefulness and extend its area of adaptation. The correct identification of Prunus