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Mark K. Ehlenfeldt and James J. Polashock

2.51 and 2.54 pg/2C, respectively. Table 2. DNA content and calculated ploidy levels of selected standards, section Hemimyrtillus species, and section Hemimyrtillus × Vaccinium corymbosum hybrids. Fertility and crossing of the parents Female

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Doina Clapa, Claudiu Bunea, Orsolya Borsai, Adela Pintea, Monica Hârța, Răzvan Ştefan, and Alexandru Fira

The genus Vaccinium is a member of the Ericaceae family, which includes ≈400 species. Of all the cultivated species and hybrids in this genus, highbush blueberry ( V. corymbosum L.) is the most commonly known species that produces small, edible

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Mark K. Ehlenfeldt and James L. Luteyn

had the potential to act as a bridge between taxonomic sections and ploidies in Vaccinium ( Ehlenfeldt et al., 2018 ). In this study, we sought to determine the feasibility of crossing 4 x V. meridionale with 4 x V. corymbosum to facilitate

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Mark K. Ehlenfeldt, Joseph Kawash, and James Polashock

cultivated blueberry and approaches to their utilization Botany 90 347 353 Ehlenfeldt, M.K. Polashock, J.J. 2014 Highly fertile intersectional blueberry hybrids of Vaccinium padifolium Section Hemimyrtillus and V. corymbosum Section Cyanococcus J. Amer

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Matthew Arrington and Lisa Wasko DeVetter

. Vicente, A. 2010 Effect of preharvest calcium applications on postharvest quality, softening and cell wall degradation of two blueberry ( Vaccinium corymbosum ) varieties Postharvest Biol. Technol. 58 98 103 Bednarska, E. 1991 Calcium uptake from the

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C.B. Ely, R.E. Frans, T.L. Lavy, R.E. Talbert, and J.D. Mattice

Two-year-old highbush blueberry bushes (Vaccinium corymbosum L. `Collins') were treated in Mar. 1985 with diuron or simazine at 2.2 or 4.5 kg a.i./ha. No residues were detected by reverse-phase high-performance liquid chromatography-ultraviolet absorbance detection (HPLC-UV) from treated berries that were harvested in June. Methiocarb was applied in May 1986 at 0.84 and 3 kg·ha-1 over the top of 3-year-old `Collins' when the berries began to ripen. Reverse-phase HPLC-UV of berries treated with methiocarb at 3 kg·ha-1 had combined residues of methiocarb and its sulfone and sulfoxide metabolites of 13.1 ppm from unrinsed and 7 ppm from rinsed berries harvested on the day of treatment; 4.9 ppm from unrinsed and 4 ppm from rinsed berries harvested 4 days after treatment; and 2.4 ppm from unrinsed and 2.5 ppm from rinsed berries harvested 8 days after treatment. Unrinsed berries treated with methiocarb at 0.84 kg·ha-1 had 5.7 ppm residue on the day of treatment and 1 ppm 8 days later. Residues from berries treated with methiocarb at 0.84 or 3 kg·ha-1 were below the legal tolerance level of 5 ppm after the required 7-day waiting period. Chemical names used: n'-(3,4-dichlorophenyl)-N,N -dimethylurea (diuron); 6-chloro- N,N' -diethyl-1,3,5-triazine-2,4-diamine (simazine); 3,5-dimethyl-4-(methylthio)phenol methylcarbamate (methiocarb).

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Gregory A. Lang and E. James Parrie

Pollen from six southern highbush blueberry cultivars derived from Vaccinium corymbosum L. and one or more other species (V. darrowi Camp, V. ashei Reade, and V. angustifolium Aiton) was incubated on nutrient agar to determine tetrad viability, pollen tube growth rates, and incidence of multiple pollen tube germinations. `Avonblue' pollen had a significantly lower tetrad germination percentage than `Georgiagem', `Flordablue', `Sharpblue', `Gulfcoast', or `O'Neal', all of which had >90% viable tetrads. The in vitro growth rate of `O'Neal' pollen tubes was significantly higher than the growth rates of `Sharpblue' and `Georgiagem pollen tubes. Of those tetrads that were viable, more than two pollen tubes germinated from 83% and 91% of the `Gulfcoast' and `Sharpblue' tetrads, respectively, while only 11% of the `Flordablue' tetrads produced more than two pollen tubes. The total number of pollen tubes germinated per 100 tetrads ranged from 157 (`Flordablue') to 324 (`Sharpblue'), resulting in actual pollen grain viabilities ranging from 39% to 81%. Genetic differences in pollen vigor, as indicated by pollen viability, pollen tube growth rates, and multiple pollen tube germinations, may influence blueberry growers' success in optimizing the beneficial effects of cross-pollination on fruit development.

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Mark K. Ehlenfeldt and Nicholi Vorsa

inheritance of this trait and an evaluation of its potential for development. Materials and Methods A parthenocarpic variant, G-176, was discovered in highbush blueberry ( Vaccinium corymbosum L.) (2n = 4x = 48) in a family of the pedigree G-105 × E-204

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Nicholi Vorsa and Richard Novy

Vaccinium darrowi (D) is a wild blueberry species with low chilling requirements for budbreak, and heat and drought tolerance. Breeding efforts to incorporate these desirable traits into cultivated blueberry (V. corymbosum) (C) would be facilitated with a better understanding of the genomic homology between the two species. An interspecific tetraploid hybrid (CCDD, 2n=4x=48) was used to evaluate genome homology and interspecific recombination. Pollen mother cells examined at diakinesis and early metaphase I exhibited an average of 4.6 chain bivalents, 11.4 ring bivalents, 1.0 chain quadrivalent, and 3.0 ring quadrivalents. This data most closely fits a chromosome pairing model in which there is a greater pairing affinity between homologues than homoeologues. An analysis of the inheritance of 14 RAPD markers unique to V. darrowi in 72 backcross progeny of the V. darrowi–corymbosum hybrid also supported the pairing model: Seven of the 14 markers deviated significantly from tetrasomic inheritance ratios, expected if chromosome pairing was totally random. On the basis of the cytogenetic and RAPD analyses, the genomes of V. darrowi and V. corymbosum are divergent from one another, with preferential pairing within genomes. This outcome suggests there may be difficulty in breaking undesirable linkages when introgressing desirable traits from V. darrowi to V. corymbosum.

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Haishan An, Jiajia Meng, Fangjie Xu, Shuang Jiang, Xiaoqing Wang, Chunhui Shi, Boqiang Zhou, Jun Luo, and Xueying Zhang

Blueberries ( Vaccinium corymbosum L.) are becoming important commercial crops because of their healthy and flavorful properties, and they can be propagated by seeds, grafting, tissue culture, and hardwood/green cuttings ( Fischer et al., 2012