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Tingting Zhao, Jingkang Hu, Yingmei Gao, Ziyu Wang, Yufang Bao, Xiaochun Zhang, Huanhuan Yang, Dongye Zhang, Jingbin Jiang, He Zhang, Jingfu Li, Qingshan Chen, and Xiangyang Xu

Station of Northeast Agricultural University. At the two- to three-leaf stage, the seedlings were used in a VIGS study. Target fragments amplification and vectors construction. Total RNA was extracted from ‘Moneymaker’ seedling leaves using the Trizol

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Lili Dong, Tongrui Liu, Di Gao, Jing Li, and Jie Qian

after germination and were grown in a growth chamber. The number of rosette branches (bud length ≥ 10 mm) and the height of the main stems were scored for phenotype analysis. Construction and transformation of virus-induced gene silencing (vigs) vector

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Hiroaki Ito, Masaki Ochiai, Hiroaki Kato, Katsuhiro Shiratake, Daigo Takemoto, Shungo Otagaki, and Shogo Matsumoto

, and winter-hardiness ( Svejda, 1974 , 1977 ). It is one of the few recurrently flowering species, which has been critically important to establishing its widespread use in the ornamental trade. VIGS is a powerful tool for the fast and efficient

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Xiaohong Wang, Bishun Ye, Xiangpeng Kang, Ting Zhou, and Tongfei Lai

-associated secondary metabolites are also rarely reported. Virus-induced gene silencing (VIGS) is an attractive reverse-genetics tool for the study of gene function in plants. Upon viral infection, plants use their innate RNAi-mediated defense machinery to target the

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Tanya J. Hall, Jennifer H. Dennis, Roberto G. Lopez, and Maria I. Marshall

federal governments on agricultural industries ( Isik, 2004 ). States can choose to set their regulations higher than the federal standard or keep it status quo with federal guidelines ( Isik, 2004 ; Kraft and Vig, 1994 ; Lester, 1994 ). Several studies

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Shanshan Seng, Jian Wu, Jiahui Liang, Fengqin Zhang, Qiuyan Yang, Junna He, and Mingfang Yi

. Silencing of GhAGPL1 by virus-induced gene silencing (VIGS) was performed as described previously ( Zhong et al., 2014 ). Cormels with 6 to 10 mm in diameter were used for VIGS assays. First, a 313-base pair (bp) specific fragment of GhAGPL1 were

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Robert L. Jarret, Gloria E. Barboza, Fabiane Rabelo da Costa Batista, Terry Berke, Yu-Yu Chou, Amanda Hulse-Kemp, Neftali Ochoa-Alejo, Pasquale Tripodi, Aniko Veres, Carolina Carrizo Garcia, Gabor Csillery, Yung-Kuang Huang, Erzsebet Kiss, Zsofia Kovacs, Mihaly Kondrak, Magda Lisette Arce-Rodriguez, Marisel A. Scaldaferro, and Antal Szoke

) . Few of the many genes reported to be involved in capsaicinoid biosynthesis have been characterized. Virus-induced gene silencing (VIGS) was used to demonstrate the participation of Kas , Comt , p Amt , and AT3 in the pathway ( Abraham-Juárez et al

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Yihua Chen, Peng Jiang, Shivegowda Thammannagowda, Haiying Liang, and H. Dayton Wilde

as a genetic model is the lack of an efficient transformation system for conducting functional genomics. There are alternative approaches being developed for the analysis of genes in peach. Virus-induced gene silencing (VIGS) is being investigated in

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Carmina Gisbert, Judith M. Dumm, Jaime Prohens, Santiago Vilanova, and John R. Stommel

, J.H. Yin, Y.X. Zhang, H.X. Chai, W.G. Gong, Z.H. 2015 VIGS approach reveals the modulation of anthocyanin biosynthetic genes by CaMYB in chili pepper leaves Front. Plant Sci. 6 500 Zhang, Y. Hu, Z. Chu, G. Huang, C. Tian, S. Zhao, Z. Chen, G. 2014

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Yuji Yamada, Masayoshi Nakayama, Hiromitsu Shibata, Sanae Kishimoto, and Takashi Ikeda

-bHLH-WDR complexes Trends Plant Sci. 20 176 185 Zenner, K. Bopp, M. 1987 Anthocyanin turnover in Sinapis alba L J. Plant Physiol. 126 475 482 Zhang, Z. Li, D.W. Jin, J.H. Yin, Y.X. Zhang, H.X. Chai, W.G. Gong, Z.H. 2015 VIGS approach reveals the modulation of