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Thyme (Thymus vulgaris L) is an important medicinal and aromatic plant used in nutrition, pharmaceutical and cosmetic industries. The main objective of this investigation was to elucidate the influence of different growning conditions on photosynthesis, chlorophyll contnet and leaf water potential in two different thyme clonal selections. Pot experiments were conducted in a greenhouse with three different soil water levels (50, 70 and 90% by weight), under supplementary lighting and natural lighting. Photosynthetic rate was positively related to soil water level and supplementary lighting. Under both light treatments, photosynthesis was found to be highest at 70% soil water level. “Selection 1” showed higher rates of photosynthetsis than “Selection 2”. “Selection 1” had slightly higher leaf water potential than “selection 2” under all growing conditions. Leaf water potential was found to be much higher for both selections grown without supplementary lighting than the variants grown under supplementary light. The mean chlorophyll content of “Selection 1” grown under supplementary lighting was found to be higher than “Selection-2” under all soil water levels (50 % 70% and 90%). There was a clear difference in leaf color between plants grown under the two light levels. This research was partially supported by Matol Botanical International.

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Cultivation of thyme for medicinal purposes should result in high dry-matter yield and sufficient active principals concentrations. In this experiment two methods of crop establishment were compared: direct sowing (final plant density: 100,000 plants/ha) and planting at two densities: D-1 (100,000 plants/ha) and D-2 (166,000 plants/ha). The use of transplants promoted growth and resulted in yields three times higher than direct sowing (3340 vs. 1002 kg dry matter/ha). There were significant differences in biomass between the two densities evaluated. Plants under D-1 weighed 33 g dry matter/plant vs. 22 g dry matter/plant for D-2. Hence, dry-matter yield per hectare was not affected by planting density. Active principals concentrations were not affected by treatments. So far, it is recommended that cultivation of thyme under Quebec's conditions be based on transplanting at moderate (100,000 plants/ha) density.

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vegetative structures per plot for Trifolium repens ( A ), Prunella vulgaris ( B ), and Thymus serpyllum ( C ) at the location with fine-textured soil and high organic matter (TROE) and the site with high sand content in soil and low organic matter

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. Litvin-Zabal (2019) reported shoot fresh and dry mass increased when increasing the DLI for hydroponically grown mint ( Mentha spp.), oregano ( Origanum vulgare ), sage ( Salvia officinalis ), and thyme ( Thymus vulgaris ). For example, the shoot fresh

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A greenhouse study was conducted from Oct. 1999 through Feb. 2000, and Mar. 2001 through Apr. 2001, to determine the potential phytotoxic effects of selected insecticides on Spanish lavender (Lavandula stoechas L.), oregano (Origanum vulgare L. `Santa Cruz'), rosemary (Rosmarinus officinalis L.), St. Johnswort (Hypericum perforatum L. `Topaz'), wolly thyme (Thymus vulgaris L. `Wolly'), and nutmeg thyme (Thymus vulgaris L. `Nutmeg'). Insecticides used for the study were Beauveria bassiana Strain GHA, pyrethrin [+ piperonyl butoxide (PBO)], azadirachtin, potassium salts of fatty acids, two rates of cinnamaldehyde, paraffinic oil, and capsaicin. Visual observations of phytotoxicity were made 7 days after the final application. Pyrethrin, potassium salts of fatty acids, and both rates of cinnamaldehyde were consistently more phytotoxic than the other insecticides. Despite the phytotoxic effects from some of the insecticides, new growth that emerged following treatments compensated for the initial damage, and the herbs were still saleable.

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With the increase in popularity of natural medicine there is an ever growing market for the production of medicinal plants. In the last decade, screening trials of a number of species were conducted. The species currently under study are: angelica (Angelica archangelica; biennial, roots harvested), thyme (Thymus vulgaris; perennial, shoot harvested), German chamomilla (Matricaria recutita; annual, flowers harvested), horehound (Marrubium vulgare; perennial, shoot harvested) and dandelion (Taraxacum officinale; considered as a biennial, roots harvested). In 1990 the species were grown on three soil types (clay-loam, sandy loam and histosol) with different fertilization and irrigation practices. In 1991 two distinct trials were undertaken. The first considered herbicide efficiency and planting density. The second dealt with «organic» management strategies. Depending on the species, treatments of compost amendment, plastic mulch and implantation techniques were compared.

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The major objective of medicinal plant production is to optimize dry matter yield as well as active ingredient concentration. This research examined the influence of three organic production systems and one conventional production system on dry matter yield and active ingredient concentration. Plants of thyme, horehound and camomile (Thymus vulgaris L., Marrubium vulgare L., Matricaria recutita [L.] Rauschter, respectivly) were sown in greenhouse and then transplanted to the field. Preliminary results showed that dry matter yield was higher with the conventional production system for all species and active ingredient concentration was higher in the organic systems for thyme and horehound. Higher active ingredient concentration in these two species was obtained in the organic-biodynamic system. For camomile, highest active ingredient concentration was found with the conventional production system. These results indicate that both dry matter yield and active ingredient concentration are affected differently by organic and conventional production systems.

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Sweet marjoram [(marjoram) Origanum majoranna], Italian parsley [(parsley) Petroselinum crispum], Summer savory [(savory) Satureja hortensis], and thyme (Thymus vulgaris) were evaluated for their yield potential during Fall–Winter–Spring (Oct.–May) 1998–99. The herbs were grown in a light sandy soil with the full-bed polyethylene mulch-micro(trickle) irrigation system. Experimental design was a split-plot replicated three times. Main plots were two N–P–K treatments: 0 N–P–K or N and K from a liquid 4–0–3.32 (N–P–K) fertilizer injected at 0.77 N and 0.64 K kg·ha–1·day–1. Sub-plots were four compost rates at 0x, 1x, 2x, and 4x (1x = 4.5 t·ha–1). Early and seasonal total yields of marjoram and savory were similar with injected N + K and 0x compost to yields with compost and with or without injected N + K fertilizer. Yields of parsley and thyme increased with increasing compost rates and were best with compost plus liquid N + K. Postharvest soil concentrations of NO3-N were lower in the parsley, than in the marjoram, savory and thyme plots. Residual concentrations of all other elements were similar with or without injected N + K or compost treatments.

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The influence of a wide range of CO2 levels on the growth, morphogenesis, and secondary metabolite production in vitro was evaluated. Shoots of thyme (Thymus vulgaris L.) and a spearmint–peppermint cross (Mentha spicata × Mentha piperita) were grown on MS medium with and without 3% sucrose under 350, 1500, 3000, 10,000, and 30,000 μL CO2/L for 8 weeks. Dichloromethane extracts from leafs were analyzed using GC-MS techniques. Prominent peaks were identified by comparison with known standards. Highest growth (i.e., fresh weight) and morphogenesis responses (i.e., leafs, shoots and roots) were obtained when shoots were grown under 10,000 μL CO2/L regardless of whether or not sucrose was included in the medium. Ultra-high CO2 concentrations (3000 μL CO2/L) stimulated secondary metabolite production regardless of whether or not the medium contained sucrose. However, the combination of certain ultra-high CO2 levels (e.g., 3000 to 10,000 μL CO2/L) and the presence of sucrose in the medium resulted in shoots producing the highest levels of secondary metabolites. These results suggest that in vitro photosynthesis, which is stimulated by ultrahigh CO2 levels, may enhance secondary metabolite production.

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Italian parsley (parsley) Petroselinum crispum, summer savory (savory) Satureja hortensis, sweet marjoram (marjoram) Origanum majoranna, and thyme Thymus vulgaris, were evaluated for their yield potential in multiple harvest during the fall–winter–spring (Dec.–May 1997–98). The herbs were grown with the full-bed polyethylene mulch-micro (trickle) irrigation system. Experimental design was a split-plot arranged in three randomized complete blocks. Main plots were two N–P–K treatments: 0 N–P–K or N and K from a liquid 4N–0P–3.32K fertilizer injected at 0.77 N and 0.64 K kg/ha per day. In the subplots, compost was applied in a 4 to 8 inches wide band on the pre-bed at 0x, 1x, 2x, and 4x rates (1x = 4.5 t·ha–1). Parsley and marjoram yields in the first three harvests and thyme yields in the first two harvests were similar with 0x compost and N + K injected fertilizers to yields with 3x and 4x compost rates with no injected N + K fertilizers. For the season, yields were higher with injected N + K fertilizers with or without compost, than in the compost treated plots with no N + K fertilizers.

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