Search Results

You are looking at 1 - 10 of 77 items for :

  • Refine by Access: All x
Clear All
Free access

Shogo Matsumoto, Kentaro Kitahara, Sadao Komori, and Junichi Soejima

S-allele genotypes of nine apple (Malus ×domestica Borkh.) cultivars were identified using S-allele–specific polymerase chain reaction (PCR)–restriction fragmentlength polymorphism (RFLP) analysis. A new S-allele, Sg, was proposed to be present in `American Summer Pearmain', `Indo', `Kitanosachi', and `Meku 10'. This allele is very similar to Sf at the nucleotide sequence (92%) and deduced amino acid sequence (94%) levels.

Free access

Ossama Kodad, Rafel Socias i Company, Ana Sánchez, and M. Margarida Oliveira

gametophytic type ( Socias i Company et al., 1976 ) and is controlled by a single S locus with multiple alleles ( Crane and Lawrence, 1929 ). To ensure that flowers may be efficiently pollinated to reach an economically acceptable fruit set ( Kester and

Free access

Rafel Socias i Company, Àngel Fernández i Martí, Ossama Kodad, and José M. Alonso

Channuntapipat, C. Sedgley, M. Collins, G. 2001 Sequences of the cDNAs and genomic DNAs encoding the S1, S7, S8 and Sf alleles from almond, Prunus dulcis Theor. Appl. Genet. 103 1115 1122 Channuntapipat, C

Free access

Sadao Komori, J. Soejima, Y. Ito, and H. Bessho

Cross-incompatible combinations among the main cultivars in apple are rarely reported in Japan. Recently, however, most new Japanese cultivars are progenies of `Ralls Janet', `Delicious', `Golden Delicious', `Jonathan', and `Indo'. Cross incompatibility in apple, therefore, will become a serious problem in the near future. Since the analysis of the S-allele genotypes were not performed, especially in Japanese apple cultivars, the fruit set percentage were examined in several combinations of `Hatsuaki' (`Jonathan' × `Golden Delicious') and `Iwakami' (`Fuji' × `Jonathan') progenies using back crossings. As a result, we found that `Golden Delicious' and `Jonathan' had no common S-allele, while `Fuji' and `Jonathan' had one common S-allele. These facts were used as basics for the S-allele genotype analysis, and fruit set percentage and seed number per fruit were investigated on a large scale. The cross seedlings between `Delicious' and `Jonathan', `Ralls Janet' and `Jonathan', `Iwakami' and `Golden Delicious', `Golden Delicious' and `Delicious', `Hatsuaki' and `Fuji', `Hatsuaki' and `Delicious', `Hatsuaki' and `Jonathan', and `Hatsuaki' and `Golden Delicious' were analyzed. In addition, incompatibility between `Redgold' and `Kinesei' (`Golden Delicious' × `Ralls Janet'), `Senshu' (`Toukou' × `Fuji') and `Iwakami', and progenies of `Northern Spy' also were analyzed. As a result, we have found the existence of six alleles and 15 genotypes, and we have established S-allele standard cultivars and strains as follows: (Sa, Sb) = `Golden Delicious'; (Sa, Sc) (4)-354, (4)-425; (Sa, Sd) = `Toukou'; (Sa, Se) = `Redgold', `Kinsei'; (Sa, Sf) = `Narihokou', (4)-4195; (Sb, Sc) = `Hatsuaki', `Kuifua', `Sekaiichi'; (Sb, Sd) = `Tsugaru', (4)-300; (Sb, Se) = (4)-150, (4)-743; (Sb, Sf) = `Northern Spy', M.9, `Umezawa'; (Sc, Sd) = `Jonathan', `Himekami'; (Sc, Sf) = `Fuji', `Shinkou'; (Sd, Se) =; (Sd, Sf) = `Senshu', `Iwakami'; (Se, Sf) = `Ralls Janet'.

Free access

Kentaro Kitahara, Junichi Soejima, Hiromitsu Komatsu, Hirokazu Fukui, and Shogo Matsumoto

The S-locus genes in the pistil (S-RNases) were cloned from the apple (Malus ×domestica Borkh.) cultivar Akane (S-genotype SdSh from pollination analysis). The Sd- and Sh-RNase corresponded to S7- and S24-RNase, which have been cloned from `Idared' and `Braeburn', respectively. Sh-RNase was very similar to Sf- and Sg-RNases at the deduced amino acid-sequence levels (93%). We developed an S-allele specific polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis method for distinguishing the Sh from Sf and Sg, and the Sh-alleles of `Akane', `Touhoku 2', `Vista Bella', and `Worcester Pearmain' were identified. We also identified the S-allele genotypes of 16 apple cultivars.

Free access

Kristi K. Barckley, Sandra L. Uratsu, Thomas M. Gradziel, and Abhaya M. Dandekar

The California almond industry is the largest supplier of almonds [Prunus dulcis (Miller) D.A. Webb] in the United States and throughout the world. Self-incompatibility is a major issue in almond production as it greatly affects nut set. In this study, we determined full-length sequences for alleles Sa - Si, determined the genotypes of 44 California cultivars, and assigned the cultivars to cross-incompatibility groups (CIGs). Newly identified S-alleles led to an increase in the number of CIGs. A pairwise distance tree was constructed using the aligned amino acid sequences showing their similarity. Four pairs of alleles (Sc and Se, Sg and Sh, Sd and Sj, and Sb and Sf) showed high sequence similarity. Because of its simplicity, reproducibility, and ease of analysis, PCR is the preferred method for genotyping S-alleles.

Free access

Shogo Matsumoto and Kentaro Kitahara

A polymerase chain reaction (PCR)-based method for identifying the S-alleles in the Asian pear [Pyrus pyrifolia (Burm) Nak.] was applied to apple (Malus ×domestica Borkh.) cultivars. With minor modifications in one of the primers, the fragments from S-genes (S-RNases) with introns were amplified from total DNA of apple cultivars possessing S2-, S3-, S5-, S7-(=Sd-), S9-(=Sc-), Sf- and Sg-allele genotypes. S-genes within S24-(=Sh-) and S26-alleles were also amplified. The PCR amplification step of this method appears to be useful for preliminary investigation of apple S-genotypes, especially for species or cultivars of unknown origin or history. Using the primers, which are a part of a new S-allele, the Se-allele encoding Se-RNase with an intron in the Se-allele was amplified. We cloned the cDNA of Se-RNase, and developed a PCR-restriction fragment length polymorphism (RFLP) analysis method for Se-allele identification. S-allele genotypes of seven apple cultivars were investigated.

Free access

Kenji Sakurai, Susan K. Brown, and Norman Weeden

The S-alleles of 55 apple (Malus ×domestica Borkh.) cultivars and selections were determined using an allele-specific polymerase chain reaction (PCR) amplification system for 11 different S-alleles (S2, S3, S4, S5, S7, S9, S24, S26, S27, Sd, Sf). Four cultivars had S-alleles different than those predicted by their parentage. Three commercial cultivars of unknown pedigrees had S-genotypes that suggested `Delicious' and `Golden Delicious' were the parents. S-genotyping results supported controlled pollination test results. The genotypes of the five triploid cultivars examined were consistent with the unreduced gamete being contributed by the female parent. Although a large number of S-genotypes is available in apple, artificial selection or repeated use of the same cultivars as parents appears to have significantly restricted the number of compatibility groups associated with commercial clones. In controlled reciprocal crosses between two cultivars of known S-genotypes, the segregation of S-genotypes and S-alleles was 1:1:1:1, the ratio expected for random pairing of alleles.

Free access

Júlia Halász, Attila Hegedűs, Zoltán Szabó, József Nyéki, and Andrzej Pedryc

fruit set, the self-incompatibility genotypes of self-incompatible (SI) tree crops have been studied intensively. Several S -alleles and incompatibility groups were described on the basis of molecular studies in sweet cherry ( Prunus avium L

Free access

Hitomi Umemura, Katsuhiro Shiratake, Shogo Matsumoto, Tsutomu Maejima, and Hiromitsu Komatsu

-compatibility of ‘Megumi’ J. Jpn. Soc. Hort. Sci. 68 236 241 Matsumoto, S. Kitahara, K. Komori, S. Soejima, J. 1999b A new S -allele in apple ‘ Sg ’, and its similarity to the ‘ Sfallele from Fuji HortScience 34 708 710 Matsumoto, S. Kitahara, K. Komatsu, H