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with close genetic relationships are difficult to screen. By contrast, SSR fingerprints have some obvious advantages, such as simplicity, clarity, and accuracy ( Li and Zheng, 2009 ; Li et al., 2009 ; Shi et al., 2007 ; Suwabe et al., 2002 ). Indeed

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, could enhance important chrysanthemum ornamental traits. Hence, development of polymorphic markers is urgently needed. Molecular markers are valuable tools used in genetic linkage map construction and MAS breeding. Among molecular markers, SSR markers

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highly reproducible, show high levels of polymorphism, and are based on polymerase chain reaction (PCR) procedures ( Korir et al. 2013 ). DNA fingerprinting by SSRs is therefore a rapid method for true-to-type assessment and variety identification. To

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discussions of grape SSRs. We thank Mark Thomas and Nigel Scott of CSIRO, Adelaide, South Australia, for sharing their SSR results, techniques, and data. Steve Kresovich and his collaborators at the USDA-ARS Plant Genetic Resources Conservation Unit, Griffin

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years to the first efforts to construct linkage maps in this species ( Lewers and Weber, 2005 ). Dossett et al. (2010 , 2012) screened more than 100 SSR primer pairs from red raspberry and blackberry ( Rubus subgenus Rubus Watson) to identify 21

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). Compared with the molecular markers mentioned above, SSR (microsatellite) markers, which are 1 to 6 bp DNA regions repeated in tandem, have desirable advantages, such as codominance, random distribution throughout the genome, a high level of polymorphisms

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addition, the transferability of SSR markers between Primula species is exceptionally low. SSR markers are considered to be effective in genetic diversity analysis, linkage, and QTL mapping; marker-assisted selection; and so on ( Rosazlina et al., 2015

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1 To whom reprint requests should be addressed. E-mail: lewersk@ba.ars.usda.gov . The authors wish to thank Ms. Kate Rappaport for SSR testing reactions and Ms. Tina Sphon for reaction analyses. Thanks also to Dr. Tad Sonstagard for managing, and

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Simple sequence repeat (SSR), also called microsatellites, are sections of DNA consisting of tandemly repeated mono-, di-, tri, tetra-, or pentanucleotide units that occur in abundance within the genomes of most eukaryotes ( Powell et al., 1996

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vegetable hybrids. With the development of expressed sequence tag-SSR and genomic SSR loci and markers in melons ( Chiba et al., 2003 ; Fernandez-Silva et al., 2008 ; Fukino et al., 2007 ; Park et al., 2013 ; Seung and Yong, 2006 ), SSR markers have been

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