compared with prickle-free red raspberry. However, prickled black raspberry stems had fewer simple trichomes compared with prickled red raspberry. Fig. 6. Scanning electron micrographs of Rubus occidentalis L. (black raspberry). ( A ) Prickles and simple
10.21273/JASHS.107.4.593 Dossett, M. 2007 Variation and heritability of vegetative, reproductive and fruit chemistry traits in black raspberry ( Rubus occidentalis L.) MS Thesis, Oregon State Univ Corvallis
Construction of black ( Rubus occidentalis ) and red ( R. idaeus ) raspberry linkage maps and their comparison to the genomes of strawberry, apple, and peach Theor. Appl. Genet. 125 311 327 Dossett, M. 2011 Evaluation of genetic diversity in wild populations of
Raspberries ( Rubus sp.) are the third most popular berry in the United States ( Geisler, 2012 ) and a growing specialty crop for both the wholesale industry and smaller, local markets, and U-pick. Postharvest susceptibility to gray mold ( Botrytis
1996, which has Rubus leucodermis Douglas ex Torr. & A. Gray in its background ( Galletta et al., 1998 ). Weber (2003) examined genetic diversity in R . occidentalis using random amplified polymorphic DNA (RAPD) markers. RAPD markers were
., 1999 ), and processing ( Zafrilla et al., 2001 ). Within Rubus , black raspberries ( Rubus occidentalis L.) have been extensively examined as potential chemopreventive agents for various forms of aerodigestive cancers ( Stoner et al., 2007 ). Early
Five polymorphic random amplified polymorphic DNA (RAPD) markers for 13 red raspberry (Rubus idaeus L.) and two purple raspberry (R. idaeus L. × R. occidentalis L.) cultivars were cloned and their termini sequenced. Sequence-specific 24-mer primer pairs were synthesized as extended RAPD primers and used in sequence characterized amplified region (SCAR) DNA analysis. All primer pairs generated polymorphic SCAR markers of the original RAPD marker sizes and length variants. Markers from four of the primer pairs could be easily scored and were adequate to identify the raspberry cultivars of the certification program of the province of Quebec.
Seeds of 25 blackberry (Rubus spp.), five red raspberry (R. idaeus L.), and two black raspberry (R. occidentalis L.) populations that had been stored for 22 to 26 years were planted in the greenhouse to evaluate their germination. Germination ranged from 0% to 84% among all populations. Thorny and thorny × thornless blackberry populations had the highest average germination; most populations had >40% germination. Thornless blackberry populations ranged from 1% to 16% germination. The seeds of two of the five red raspberry populations did not germinate and none of the black raspberry seeds germinated.
Abstract
‘Royalty’ is a new purple raspberry cultivar with the high fruit quality of the red raspberry (Rubus idaeus L.) and the vigor and large fruit size associated with the purple raspberry (R. idaeus × R. occidentalis L). The cultivar is hardy and has wide adaptation and good yield potential. It has immunity to Amphorophora agathonica Hottes, the aphid vector of the raspberry mosaic virus complex, and resistance to Aphis rubicola Oestland, the aphid vector of raspberry leaf curl. It also shows resistance to Byturus rubi Barber, the raspberry fruit worm.
Interest in molecular markers and genetic maps is growing among researchers developing new cultivars of Rubus L. (raspberry and blackberry). Several traits of interest fail to express in seedlings or reliably in some environments and are candidates for marker-assisted selection. A growing number of simple sequence repeat (SSR) molecular markers derived from Rubus and Fragaria L. (strawberry) are available for use with Rubus mapping populations. The objectives of this study were to test 142 of these SSR markers to screen raspberry and blackberry parental genotypes for potential use in existing mapping populations that segregate for traits of interest, determine the extent of inter-species and inter-genera transferability with amplification, and determine the level of polymorphism among the parents. Up to 32 of the SSR primer pairs tested may be useful for genetic mapping in both the blackberry population and at least one of the raspberry populations. The maximum number of SSR primer pairs found useable for mapping was 60 for the raspberry population and 45 for the blackberry population. Acquisition of many more nucleotide sequences from red raspberry, black raspberry, and blackberry are required to develop useful molecular markers and genetic maps for these species. Rubus, family Rosaceae, is a highly diverse genus that contains hundreds of heterozygous species. The family is one of the most agronomically important plant families in temperate regions of the world, although they also occur in tropical and arctic regions as well. The most important commercial subgenus of Rubus is Idaeobatus Focke, the raspberries, which are primarily diploids. This subgenus contains the european red raspberry R. idaeus ssp. idaeus L., as well as the american black raspberry R. occidentalis L. and the american red raspberry R. idaeus ssp. strigosus Michx. Interspecific hybridization of these, and other raspberry species, has led to greater genetic diversity and allowed for the introgression of superior traits such as large fruit size, fruit firmness and quality, disease resistance, and winter hardiness.