use of these species is found in the literature. The use of wild Mediterranean plant species, including Rosmarinus officinalis , has increased and may be an interesting solution because of their good resistance to adverse environmental conditions
Regeneration from callus of rosemary has not been reported. Leaf segment, meristem-tip and shoot-tip explants of Rosmarinus officinalis were cultured on a Murashige and Skoog (MS) medium supplemented with five concentrations of the cytokinin thidiazuron (TDZ) alone or in combination with 3-indoleacetic acid (IAA). Callus was formed on the base and leaves of the shoottips after 6 weeks when cultured under cool white fluorescent light (26 u mol·S-1 m-2) on MS containing 0.5, 1.0, 1.5 or 2.0 mg/l TDZ. Calti were transferred to fresh MS medium supplemented with 0.2, 0.4, 0.6, 0.8 or 1.0 mg/l TDZ or 2.0, 4.0, 6.0 or 8.0 mg/l benzyladenine (BA) where shoot formation occurred. Essentiality of IAA was not clear from these experiments and further research is underway to refine regeneration protocol
able to survive for 2 weeks which was expected due to the shallow depth of the green roof substrate layer. Rosmarinus officinalis. The slope of the moisture curve reduction of the nonirrigated plants was similar to that of helichrysum and Greek
of drying conditions and extraction protocols on total phenolic content and antioxidant capacity of selected herbs. Materials and Methods Plant materials, harvesting, and drying. Three herbs, rosemary ( Rosmarinus officinalis ), motherwort ( Leonurus
formaldehyde/cm 2 of leaf area over 5 h and was the most effective of the 86 species tested. In contrast, D. deremensis was the least effective. Of the herbs, Lavandula spp., Pelargonium spp., and Rosmarinus officinalis were the most effective in
maintained their growth and visual quality even at a 24% ET o irrigation application. Groundcovers, Ruschia lineolate nana, Rosmarinus officinalis ‘Roman Beauty’, and Eremphila glabra have the potential to perform well with ≥49% ET o irrigation
, Israel), and rosemary ‘Arp’ ( Rosmarinus officinalis ) (Dümmen Orange NA, Inc., Columbus, OH) were grown in 15.3-cm (1.3-L; The HC Companies, Twinsburg, OH) round containers filled with a 70% peat, 21% perlite, and 9% vermiculite mixture (Suremix
use water to produce biomass. We selected three important horticultural species for which water requirements have not been quantified. Rosemary ( Rosmarinus officinalis ) is an evergreen shrub/subshrub native to the Mediterranean region that is widely
Rosemary Rosmarinus officinalis is a member of the Lamiaceae. Rosmarinic acid (RA) is a very strong antioxidant produced in the chloroplast, and used to protect plant tissues against oxidative stress. A number of investigations showed that the sucrose concentration in the callus growing medium greatly influenced the production of secondary metabolites of the phenylpropanoid pathway such as RA. The aim of this study was to test the effect of elevated sucrose concentrations (2%, 3%, 4%, 5%, and 6% sucrose) and the effect of light and dark treatments on the production of RA in the callus of five different genotypes. The genotypes were Majorca, Rosmarinus officinalis, Pine Scented, Madeline Hill, and APR. It was found that the dark treatment produces more RA than the light treatment in all genotypes, and in all sucrose concentrations. The RA concentration increased with increasing the sucrose concentration from 2%—reaching the highest concentration at 4% and 5% in most genotypes. The RA concentration declined again at 6% sucrose in all genotypes. We concluded that for the extraction of RA from rosemary callus it is preferred to be produced in the dark—this will save energy and will produce more RA than the light treatment. Also it is preferred to use sucrose concentration at 4% for genotypes Rosmarinus officinalis, Pine Scented, and APR; and 3% sucrose for genotype Madeline Hill in the dark condition. While for the light condition, it is preferred to use 5% sucrose with genotypes Majorca, Rosmarinus officinalis, Pine Scented, and Madeline Hill; and 4% sucrose for genotype APR.