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Matthew D. Robbins, Mohammed A.T. Masud, Dilip R. Panthee, Randolph G. Gardner, David M. Francis, and Mikel R. Stevens

total of 1152 F 2 plants were subjected to DNA marker analysis to identify recombinants between Ph-3 and Sw-5 . Eleven recombinant F 2 plants were identified with the genes putatively in the coupling phase and were self-pollinated to create F 3

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Ji-Jhong Chen, Jeanette Norton, Heidi Kratsch, Youping Sun, and Larry Rupp

receive the soil topdressing treatment. The plants with N treatment were irrigated with quarter-strength N-free Hoagland’s solution ( Hoagland and Arnon, 1950 ) with 2 m m NH 4 NO 3 at pH 6.5; those without N treatment were irrigated with the same

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Laura J. Chapin, Youyoun Moon, and Michelle L. Jones

were incubated for 2–3 h at 37 °C or up to 6 h at 30 °C. After the flasks were put on ice for 5 min, the cells were harvested by centrifugation at 5000 g n for 5 min at 4 °C. The cells were resuspended in a 0.25 × volume of cold 20 m m Tris-HCl pH 8

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Zhuping Fan, Yike Gao, Ling Guo, Ying Cao, Rong Liu, and Qixiang Zhang

hybrids produced by I. spuria and seven I. germanica cultivars, and a relatively high broad-sense heritability was observed for PH, standard width, standard height, flower size, and leaf width (LW). In our previous research on bearded iris, WF and the

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Qianqian Shi, Long Li, Lin Zhou, and Yan Wang

m m MES, 8 μL of 1 m m CaCl 2 , 7 μL of 5 m m β-mercaptoethanol, and 0.02 g of 0.1% (w/v) BSA; pH 5.7). After vacuum infiltration for 30 min without shaking, the enzymolysis solution with petal strips was shaken for 1 h at 80 rpm and 25 °C to

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R.J. Griesbach

The biochemistry of flowers is very complex, depending not only on the specific anthocyanin present but also on vacuolar pH, presence of metal ions, type of co-pigment present, and the molar ratio of co-pigment to anthocyanin. Because of the wide array of different flower colors, Petunia hybrida is an excellent model system to study the genetic interaction of all of these factors. The segregation of the different flower colors in an F2 population from a red × violet outcross could be explained through the combined inheritance of anthocyanin pigmentation and pH. The inheritance of anthocyanin pigmentation was controlled by two independent genes (hf and Mf) that followed simple Mendelian genetics. The inheritance of pH was more complex, being controlled by two independent co-dominant genes (Ph1 and Ph2). Linkage of the various pH and anthocyanin genes prevented the expression of all of the potential gene combinations.

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Jon T. Lindstrom, Chih-Hsien Lei, Michelle L. Jones, and William R. Woodson

1 Current address: Department of Horticulture, University of Arkansas, Fayetteville, AR 72701. 2 Current address: Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO 80523-1173. 3 To whom reprint

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Dilip R. Panthee

oxysporum f.sp. lycopersici (Sacc.) Snyd. and Hans.) (races 1 and 2) ( I/I and I-2/I-2 genes), late blight (LB) ( Ph-2/ph-2 and Ph-3/ph-3 genes), Tomato mosaic virus (ToMV) ( Tm2/tm2 gene), and Tomato spotted wilt virus (TSWV) ( Sw-5/sw-5 gene

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Dilip R. Panthee and Randy G. Gardner

‘Mountain Bebe’ is the F 1 hybrid of NC 7 Grape × NC 8 Grape. It is resistant to late blight ( Ph-2 and Ph-3 genes), tomato spotted wilt virus ( Sw-5 gene), and fusarium wilt races 2 and 3 ( I-2 and I-3 genes). The hybrid has a compact

Open access

E. Kopeliovitch, Y. Mizrahi, H. D. Rabinowitch, and N. Kedar

Abstract

Organoleptic tests of the non-ripening tomato mutants rin and nor and their F1 hybrids with the normal-fruit-bearing cultivar ‘Rutgers’ indicated that fruits of the rin heterozygous plants (rin/+) were slightly inferior and that those of inferior in flavor to fruits of the normal genotype (+/+), all sampled 3–5 days after ethylene and CO2 evolution rates attained maximum levels. The flavor of fruits of the double heterozygote nor heterozygotes (nor/+) were distinctly rin/+, nor/+ was poorer than either of the 2 single-gene heterozygotes, while fruits of both homozygous plants, nor/nor and rin/rin, were unpalatable. Analyses of pH, titratable acidity, total soluble solids, and reducing sugars did not indicate that any of these parameters is responsible for the inferior flavor of the genotypes containing the non-ripening genes. Comparisons of reciprocal crosses provided no evidence of cytoplasmic inheritance of fruit flavor.